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acumen explorer ex3 laser scanning microplate cytometer  (SPT Labtech)


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    Structured Review

    SPT Labtech acumen explorer ex3 laser scanning microplate cytometer
    Acumen Explorer Ex3 Laser Scanning Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acumen+microplate+cytometer/bio_rxiv__2023__09__06__556522-267-25-32
    Average 86 stars, based on 1 article reviews
    acumen explorer ex3 laser scanning microplate cytometer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Cytometry:

    Article Title: Small Molecule Screening with Laser Cytometry Can Be Used to Identify Pro-Survival Molecules in Human Embryonic Stem Cells
    Article Snippet: .. In all cases, data were acquired using an Acumen microplate cytometer (TTP Labtech). .. For quantification of GFP signal from the OCT4-GFP reporter, culture medium was aspirated and replaced with Dulbecco’s phosphate buffered saline (dPBS, Life Technologies), after which plates were immediately transferred to the Acumen system.

    Article Title: Antibodies and methods for treating estrogen receptor-associated diseases
    Article Snippet: .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech). .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech).

    Incubation:

    Article Title: Antibodies and methods for treating estrogen receptor-associated diseases
    Article Snippet: .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech). .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech).

    Staining:

    Article Title: Antibodies and methods for treating estrogen receptor-associated diseases
    Article Snippet: .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech). .. After 2.0 hours incubation at 37° C., the plates were washed twice with PBS, pH 7.2, and then 15 μl/well of Alexa488 conjugated goat anti-mouse IgG (F(ab′)2 fragment, Invitrogen) was added following with 1.0 hour incubation at 37° C. The plates were then washed 3-4 times with PBS and the cells were then fixed with 4% Paraformaldehyde (Sigma) and stained again with PI (DOJINDO) for 1.0 hour at 37° C. The assay plates were read with Acumen microplate cytometer (Acumen eX3, TTP LabTech).



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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
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    SPT Labtech microplate laser scanning cytometer acumen ex3
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    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen <t>microplate</t> cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.
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    Image Search Results


    Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen microplate cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.

    Journal: PLoS ONE

    Article Title: Small Molecule Screening with Laser Cytometry Can Be Used to Identify Pro-Survival Molecules in Human Embryonic Stem Cells

    doi: 10.1371/journal.pone.0054948

    Figure Lengend Snippet: Three treatments are compared to assess changes in survival of dissociated hESCs: 0.1% DMSO (negative control; A,D ), 10 µM HA-1077 (positive control; B,E ), and 10 µM AST 5588603 (representative of small molecules identified in OCT4-GFP screening assay; C,F ). A-C: Readout from the Acumen microplate cytometer. The wells treated with HA-1077 and AST 5588603 had similar numbers of GFP positive objects (quantified in G ). D-F: Alkaline phosphatase staining of cells treated as described. Pluripotent cells are stained with a red color. Wells treated with HA-1077 ( E ) contain significantly more pluripotent cells than cells treated with DMSO or any other candidate compounds. G: Quantification of GFP signal detected in ( A- C). H: Quantification of alkaline phosphatase staining in ( D-F ) by calculating what percent of the well area stained positive for alkaline phosphatase. Data shown as mean ±SD, ***: p<0.001.

    Article Snippet: In all cases, data were acquired using an Acumen microplate cytometer (TTP Labtech).

    Techniques: Negative Control, Positive Control, Screening Assay, Cytometry, Staining