Journal: Bioactive Materials

Article Title: Mild photothermal-responsive hydrogel with H 2 Se delivery for anti-infection and microenvironment remodeling to bone regeneration in diabetic osteomyelitis

doi: 10.1016/j.bioactmat.2026.04.041

Figure Lengend Snippet: Characterization of TF@GL hydrogel. (A) Schematic diagram of the synthetic lipoic acid grafted gelatin, (B) TF@GL sol, and (C) TF@GL microgel hydrogel. (D) Photographs of (a) microgel stained with rhodamine B (Scale bars: 100 μm), (b) two colors of Gel-LA microgels forming complete hydrogels after UV irradiation, (c) healing of two Gel-LA hydrogels after UV irradiation. (E) SEM images showing the microstructures of GL and TF@GL microgel hydrogels. Scale bars: 500 μm, 200 μm, and 100 μm. (F) Relationship between sol-gel transition and photocrosslinking time, (G) Frequency scanning rheology (0.1-10 Hz, 1% strain), (H) Rotational strain scanning rheology (0.01-10000% strain, 1 Hz), (I) Stress-strain curves of GL and TF@GL microgel-based hydrogel. G': storage modulus; G": loss modulus. (J) DPPH scavenging by hydrogel and microgel-based hydrogel of Gel-MA and Gel-LA (n = 3). (K) Degradation of Gel-LA microgel-based hydrogel in PBS containing Collagenase type I and H 2 O 2 (0, 50 μM, 200 μM) (n = 3). (L) The thermal images of hydrogels under NIR irradiation (808 nm, 1.2 W cm −2 ). Data are presented as mean ± SD; ∗∗∗ P < 0.001.

Article Snippet: Gelatin and carbonyldiimidazole (CDI) was obtained from the Sigma-Aldrich (Merck KGaA, Darmstadt, Germany), FeCl 3 ·6H 2 O, urea, sodium citrate, sodium polyacrylate, gelatin, Lipoic acid, Woolins' reagent, anhydrous dichloromethane, morpholine, Dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China), Sodium selenite (Na 2 SeO 3 ) and selenium (Se) powder were obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China).

Techniques: Staining, Irradiation