Journal: PLoS ONE

Article Title: Singlet Oxygen Induced Products of Linoleates, 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids (HODE), Can Be Potential Biomarkers for Early Detection of Type 2 Diabetes

doi: 10.1371/journal.pone.0063542

Figure Lengend Snippet: Abbreviations: a, Blood collection; b, Glycometabolism Markers (glucose, insulin, leptin, adiponectin, IL-6, TNF-α); c, Oxidative stress markers (9-(Z,E)-HODE, 9-(E,E)-HODE, 12-(Z,E)-HODE, 10-(Z,E)-HODE, 13-(Z,E)-HODE, 13-(E,E)-HODE, 5HETE, 12HETE, 15HETE, isoPs, linoleic acid, total cholesterol, 7β-hydroxycholesterol).

Article Snippet: 9-(E,E)-HODE, 13-(E,E)-HODE, 10-(Z,E)-HODE, and 12-(Z,E)-HODE were obtained from Larodan Fine Chemicals AB (Malmo, Sweden).

Techniques:

Journal: PLoS ONE

Article Title: Singlet Oxygen Induced Products of Linoleates, 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids (HODE), Can Be Potential Biomarkers for Early Detection of Type 2 Diabetes

doi: 10.1371/journal.pone.0063542

Figure Lengend Snippet: (a) Total ion chromatogram, (b) 9-(Z,E)- and (E,E)-HODE, (c) 10- and 12-(Z,E)-HODE, (d) 13-(Z,E)- and (E,E)-HODE, (e) 13-(Z,E)-HODE-d 4 , (f) 5-HETE, (g) 12-HETE, (h) 15-HETE, (i) 8–iso-PGF 2α , and (j) 8-iso-PGF 2α -d 4.

Article Snippet: 9-(E,E)-HODE, 13-(E,E)-HODE, 10-(Z,E)-HODE, and 12-(Z,E)-HODE were obtained from Larodan Fine Chemicals AB (Malmo, Sweden).

Techniques:

Journal: PLoS ONE

Article Title: Singlet Oxygen Induced Products of Linoleates, 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids (HODE), Can Be Potential Biomarkers for Early Detection of Type 2 Diabetes

doi: 10.1371/journal.pone.0063542

Figure Lengend Snippet: Correlation between fasting plasma levels of (10- and 12-(Z,E)-HODE)/LA in subjects without diabetic type and the insulinogenic index (A) or Matsuda Index 3 (B) during OGTT.

Article Snippet: 9-(E,E)-HODE, 13-(E,E)-HODE, 10-(Z,E)-HODE, and 12-(Z,E)-HODE were obtained from Larodan Fine Chemicals AB (Malmo, Sweden).

Techniques:

Journal: bioRxiv

Article Title: Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma

doi: 10.1101/752519

Figure Lengend Snippet: A. FUT4 mRNA levels in CL1-5 lung cancer cells transfected with FUT4 shRNA (#751, #753, #792) measured by quantitative real-time PCR. B. Dot plots showing relative invasion ability of CL1-5 cells with FUT4 knockdowns measured by matrigel-based transwell invasion assays. Representative images of cells with DAPI nuclear stains in the lower chambers are shown. C. Dot plots showing relative migration ability of CL1-5 cells with FUT4 knockdowns. Relative migration ability was calculated using the cell number in the lower chamber of the transwell system for each clone compared to that of the scramble control. Representative images of cells with DAPI nuclear stains in the lower chambers are shown. D. Numbers of lung cancer cells with FUT4 knockdowns (CL1-5_shFUT4) adhered to the vascular walls or retained in the lung tissues following intracardial injection visualized under Zeiss Axio Observer microscope. Scale bar: 5 mm. p value was calculated by Mann-Whitney test. *** p <0.0005. E. Numbers of metastatic foci in the lungs of nude mice at 28 days after tail vein injection of lung cancer cells with FUT4 knockdown (CL1-5_shFUT4). Pictures of lung nodules from one representative mouse in each group are shown in the right panel . F. Diagram of in vivo lung cancer metastasis assay via tail vein injection in nude mice subject to treatments with EGFR or TGF-β inhibitors. G. Dot plots showing relative metastatic abilities of A549_vector or A549_FUT4 high lung cancer cells in nude mice receiving 0.25 mg/kg Afatinib or 15 mg/kg LY2157299 treatment. Numbers of metastatic foci in the lungs were counted and normalized to the numbers in the mock (normal saline)-treated groups. Data information: p value in (A, B, C, G) was calculated by one-way ANOVA with Dunnett’s test. * p <0.05, ** p < 0.01, *** p <0.0005, and in (D, E) was calculated by Mann-Whitney test. ns: not significant. Data in (A-F) were performed in three biological replicates and presented as mean ± standard errors.

Article Snippet: Drug treatment assay: A549 (FUT4 high and vector control) human lung cancer cells were injected into the tail vein of 6-week-old nude mice with 5*10 cells per mouse to establish lung cancer metastatic model. Mice were intraperitoneally injected with DMSO (mock control), afatinib (0.25 mg/Kg BW) (TargetMol TM-T2510) and LY2157299 (15 mg/Kg BW) (TargetMol TM-T2303) three times per week from week 2 to week 7 post-injection to treat metastatic lung tumor.

Techniques: Transfection, shRNA, Real-time Polymerase Chain Reaction, Migration, Injection, Microscopy, MANN-WHITNEY, In Vivo, Plasmid Preparation