Journal: Frontiers in Plant Science

Article Title: Lanthanum enhances biomass and bioactive metabolite production in Glycyrrhiza uralensis via coordinated gene regulation

doi: 10.3389/fpls.2025.1696545

Figure Lengend Snippet: Metabolomics analysis of Glycyrrhiza uralensis secondary metabolites. (A) Metabolomics study design; (B) Glycyrrhiza uralensis and its major secondary metabolites; (C) Chromatograms of six constituents in Glycyrrhiza uralensis with internal standard; (D) Contents of six main secondary metabolites of Glycyrrhiza uralensis (D1) : Glycyrrhizic acid; (D2) : Glycyrrhetinic acid; (D3) : Liquiritin; (D4) : Liquiritigenin; (D5) : Isoliquiritin (D6) : Isoliquiritigenin). Data are presented as mean ± SD (n = 3). Significant differences between W and La groups were determined by Student's t-test (** p < 0.01, *** p < 0.001).

Article Snippet: Certified reference standards of glycyrrhizic acid (purity ≥98%, Shanghai Yuanye), liquiritin (≥98%, Shanghai Yuanye), liquiritigenin (≥98%, Shanghai Yuanye), isoliquiritigenin (≥96%, Shanghai Yuanye), isoliquiritin (≥98%, Shanghai Yuanye), and glycyrrhetinic acid (≥98%, Shanghai Yuanye) were accurately weighed (0.00146 g, 0.00103 g, 0.00203 g, 0.00286 g, 0.00242 g, and 0.00160 g, respectively), dissolved in HPLC-grade methanol (Merck), and serially diluted to prepare stock solutions (1 mg/mL).

Techniques:

Journal: Frontiers in Plant Science

Article Title: Lanthanum enhances biomass and bioactive metabolite production in Glycyrrhiza uralensis via coordinated gene regulation

doi: 10.3389/fpls.2025.1696545

Figure Lengend Snippet: Integrated correlation network of bioactive metabolites, biosynthetic genes, and growth traits in Glycyrrhiza uralensis under La treatment. (A) Glycyrrhizic acid biosynthesis pathway; (B) Liquiritin, liquiritigenin, isoliquiritigenin, and isoliquiritin biosynthesis pathway; (C) Correlation analysis of bioactive metabolites and biosynthetic genes; (D) Correlation Analysis of Bioactive Metabolites and Growth Traits; (E) Co-occurrence network displaying the correlation values between physiological growth parameters, metabolites and differential genes. Size of the node indicates the degree of these variables. Solid lines indicate significant correlations ( p < 0.05), while dashed lines denote non-significant correlations ( p ≥ 0.05).

Article Snippet: Certified reference standards of glycyrrhizic acid (purity ≥98%, Shanghai Yuanye), liquiritin (≥98%, Shanghai Yuanye), liquiritigenin (≥98%, Shanghai Yuanye), isoliquiritigenin (≥96%, Shanghai Yuanye), isoliquiritin (≥98%, Shanghai Yuanye), and glycyrrhetinic acid (≥98%, Shanghai Yuanye) were accurately weighed (0.00146 g, 0.00103 g, 0.00203 g, 0.00286 g, 0.00242 g, and 0.00160 g, respectively), dissolved in HPLC-grade methanol (Merck), and serially diluted to prepare stock solutions (1 mg/mL).

Techniques: