Review



accessory plasmid ap selection system papzif268  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc accessory plasmid ap selection system papzif268
    Accessory Plasmid Ap Selection System Papzif268, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/Accessory+plasmid+2+(Plasmid+%23128571)/pm33134656__ao0c03508_si_001-10-2-13
    Average 93 stars, based on 3 article reviews
    accessory plasmid ap selection system papzif268 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii
    Article Snippet: .. 144 Generation of plasmid variants containing tellurite resistance 145 Additional variants of each plasmid containing an additional tellurite resistance cassette for use in multi-146 drug resistance A. baumannii strains are also available under ‘Accessory plasmids’ on the Kit’s Addgene 147 webpage. ..

    other:

    Article Title: Genetically Engineered Human Kidney Cells for Real-Time Cytotoxicity Testing In Vitro
    Article Snippet: We prepared a pHRed bacterial glycerol stock by transforming One Shot Stbl3 E. coli cells (Thermo Fisher, Waltham, MA) with FUG-W-pHRed DNA, following the manufacturer’s instructions.

    Gene Expression:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..

    Expressing:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..

    Mutagenesis:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..

    Multiple Displacement Amplification:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..

    Clone Assay:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..

    Transfection:

    Article Title: A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism.
    Article Snippet: Lipofectamine-3000 (#L3000015, Invitrogen) was used following the protocol provided by the manufacturer, and cells were harvested 24–48 h post-transfection. .. Lentiviral-mediated ectopic gene expression The expression of the HA-tagged p110a (wild type), C2 deletion mutant (p110a C2 Del.) and p110a 4Q mutant in MDA-MB-231 cells were carried out by lentiviral-mediated ectopic gene expression as described previously.47 Briefly, for the generation of the infectious viral particles, cDNAs for the indicated contructs cloned into pWPT-GFP lentiviral vector were transfected along with the accessory plasmids, psPAX2 and pMD2.G (Addgene) into HEK 293T cells using calcium phosphate. ..



    Similar Products

    86
    TaKaRa accessory plasmid ptofa
    Accessory Plasmid Ptofa, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pm37944824-174-26-30
    Average 86 stars, based on 1 article reviews
    accessory plasmid ptofa - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    93
    Addgene inc accessory plasmid ap selection system papzif268
    Accessory Plasmid Ap Selection System Papzif268, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/Accessory+plasmid+2+(Plasmid+%23128571)/pm33134656__ao0c03508_si_001-10-2-13
    Average 93 stars, based on 1 article reviews
    accessory plasmid ap selection system papzif268 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc accessory plasmid pjpc12 δps prmb0034 genevi
    Accessory Plasmid Pjpc12 δps Prmb0034 Genevi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pJPC12-%CE%94M13-PRM-B0034-geneVI+(Plasmid+%2380858)/pm39462751-184-13-16
    Average 93 stars, based on 1 article reviews
    accessory plasmid pjpc12 δps prmb0034 genevi - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Addgene inc plasmids for the originally used constructs of accessory proteins
    Plasmids For The Originally Used Constructs Of Accessory Proteins, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/plasmids+for+the+originally+used+constructs+of+accessory+proteins/pm38969281-56-8-22
    Average 90 stars, based on 1 article reviews
    plasmids for the originally used constructs of accessory proteins - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Addgene inc accessory protein expression vectors
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Protein Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/Accessory+plasmid+2+(Plasmid+%23128571)/pmc11191075-143-3-10
    Average 93 stars, based on 1 article reviews
    accessory protein expression vectors - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Addgene inc accessory plasmids
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/Accessory+plasmid+2+(Plasmid+%23128571)/pm38630589-290-60-65
    Average 93 stars, based on 1 article reviews
    accessory plasmids - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Addgene inc plasmid encoding hiv 1 tat accessory protein
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Plasmid Encoding Hiv 1 Tat Accessory Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pOX-twk16+(Plasmid+%2316444)/pmc10973674-100-26-32
    Average 94 stars, based on 1 article reviews
    plasmid encoding hiv 1 tat accessory protein - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Addgene inc plasmid encoding hiv 1 rev accessory protein
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Plasmid Encoding Hiv 1 Rev Accessory Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pOX-twk16+(Plasmid+%2316444)/pmc10973674-100-35-41
    Average 94 stars, based on 1 article reviews
    plasmid encoding hiv 1 rev accessory protein - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    86
    TaKaRa accessory plasmid
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pm37832876-199-10-13
    Average 86 stars, based on 1 article reviews
    accessory plasmid - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    90
    PlasmidFactory gmbh accessory capsid plasmid pdp9
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Capsid Plasmid Pdp9, supplied by PlasmidFactory gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/accessory+plasmids/pdp9/pmc10905335-29-15-19
    Average 90 stars, based on 1 article reviews
    accessory capsid plasmid pdp9 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Journal: ACS Omega

    Article Title: SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways

    doi: 10.1021/acsomega.4c02631

    Figure Lengend Snippet: SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Article Snippet: SARS-CoV-2 nonstructural and accessory protein expression vectors were purchased from Addgene (MA, USA): pTwist-CMV-Nsp9–3xFlag (pGBW- m4133209), pTwist-CMV-ORF6–3xFlag (pGBW-m4252831), and pTwist-CMV-ORF3a- 3xFlag (pGBW-m4133902).

    Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Control