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ac-devd-afc substrate  (Cayman Chemical)


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    Structured Review

    Cayman Chemical ac-devd-afc substrate
    Ac Devd Afc Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/pm38716700-304-30-31
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Negative Control:

    Article Title: Cell Death Triggered by the Autophagy Inhibitory Drug 3-Methyladenine in Growing Conditions Proceeds With DNA Damage
    Article Snippet: The activity of effector caspases (DEVDase activity) was obtained by quantifying the fluorescence released from Ac-DEVD-afc substrate (Cayman chemical, Ann Arbor, MI, USA).

    Article Title: Ruthenium(II) polypyridyl complexes with benzoxazole derivatives and non-innocent ligands as effective antioxidants in human neuroblasts.
    Article Snippet: Ruthenium(II) polypyridyl complexes continue to raise increasing interest for the encouraging results in several biomedical areas.. Considering their vast chemical-physical repertoire, in particular the possibility to switch from the sensitization of reactive oxygen species (ROS) to ROS-scavenging abilities by tuning the nature of their ligands, it is therefore surprising that their potential as antioxidants has not been largely investigated so far.. Herein, we explored the antioxidant behaviour of the novel ruthenium compound [Ru(dbpy)(2,3-DAN)Cl]PF6 (Ru1), featuring a benzoxazole derivative (dpby = 2,6-bis(4-methyl-2-benzoxazolyl)pyridine) and the noninnocent 2,3-diamminonaftalene (2,3-DAN) ligand, along with the reference tpy-containing analogue [Ru(tpy)(2,3-DAN)Cl]PF6 (Ru2) (tpy = 2,2’:6’,2’’-terpyridine).

    Incubation:

    Article Title: Cell Death Triggered by the Autophagy Inhibitory Drug 3-Methyladenine in Growing Conditions Proceeds With DNA Damage
    Article Snippet: The activity of effector caspases (DEVDase activity) was obtained by quantifying the fluorescence released from Ac-DEVD-afc substrate (Cayman chemical, Ann Arbor, MI, USA).

    Article Title: Ruthenium(II) polypyridyl complexes with benzoxazole derivatives and non-innocent ligands as effective antioxidants in human neuroblasts.
    Article Snippet: Ruthenium(II) polypyridyl complexes continue to raise increasing interest for the encouraging results in several biomedical areas.. Considering their vast chemical-physical repertoire, in particular the possibility to switch from the sensitization of reactive oxygen species (ROS) to ROS-scavenging abilities by tuning the nature of their ligands, it is therefore surprising that their potential as antioxidants has not been largely investigated so far.. Herein, we explored the antioxidant behaviour of the novel ruthenium compound [Ru(dbpy)(2,3-DAN)Cl]PF6 (Ru1), featuring a benzoxazole derivative (dpby = 2,6-bis(4-methyl-2-benzoxazolyl)pyridine) and the noninnocent 2,3-diamminonaftalene (2,3-DAN) ligand, along with the reference tpy-containing analogue [Ru(tpy)(2,3-DAN)Cl]PF6 (Ru2) (tpy = 2,2’:6’,2’’-terpyridine).

    Activity Assay:

    Article Title: Cell Death Triggered by the Autophagy Inhibitory Drug 3-Methyladenine in Growing Conditions Proceeds With DNA Damage
    Article Snippet: The activity of effector caspases (DEVDase activity) was obtained by quantifying the fluorescence released from Ac-DEVD-afc substrate (Cayman chemical, Ann Arbor, MI, USA).

    Article Title: Ruthenium(II) polypyridyl complexes with benzoxazole derivatives and non-innocent ligands as effective antioxidants in human neuroblasts.
    Article Snippet: Ruthenium(II) polypyridyl complexes continue to raise increasing interest for the encouraging results in several biomedical areas.. Considering their vast chemical-physical repertoire, in particular the possibility to switch from the sensitization of reactive oxygen species (ROS) to ROS-scavenging abilities by tuning the nature of their ligands, it is therefore surprising that their potential as antioxidants has not been largely investigated so far.. Herein, we explored the antioxidant behaviour of the novel ruthenium compound [Ru(dbpy)(2,3-DAN)Cl]PF6 (Ru1), featuring a benzoxazole derivative (dpby = 2,6-bis(4-methyl-2-benzoxazolyl)pyridine) and the noninnocent 2,3-diamminonaftalene (2,3-DAN) ligand, along with the reference tpy-containing analogue [Ru(tpy)(2,3-DAN)Cl]PF6 (Ru2) (tpy = 2,2’:6’,2’’-terpyridine).

    Fluorescence:

    Article Title: Cell Death Triggered by the Autophagy Inhibitory Drug 3-Methyladenine in Growing Conditions Proceeds With DNA Damage
    Article Snippet: The activity of effector caspases (DEVDase activity) was obtained by quantifying the fluorescence released from Ac-DEVD-afc substrate (Cayman chemical, Ann Arbor, MI, USA).

    Article Title: Ruthenium(II) polypyridyl complexes with benzoxazole derivatives and non-innocent ligands as effective antioxidants in human neuroblasts.
    Article Snippet: Ruthenium(II) polypyridyl complexes continue to raise increasing interest for the encouraging results in several biomedical areas.. Considering their vast chemical-physical repertoire, in particular the possibility to switch from the sensitization of reactive oxygen species (ROS) to ROS-scavenging abilities by tuning the nature of their ligands, it is therefore surprising that their potential as antioxidants has not been largely investigated so far.. Herein, we explored the antioxidant behaviour of the novel ruthenium compound [Ru(dbpy)(2,3-DAN)Cl]PF6 (Ru1), featuring a benzoxazole derivative (dpby = 2,6-bis(4-methyl-2-benzoxazolyl)pyridine) and the noninnocent 2,3-diamminonaftalene (2,3-DAN) ligand, along with the reference tpy-containing analogue [Ru(tpy)(2,3-DAN)Cl]PF6 (Ru2) (tpy = 2,2’:6’,2’’-terpyridine).



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    Image Search Results


    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test