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Effects of Que on cell viability, mRNA levels, apoptosis and protein expression in MCF-7 cells. (A) Cell viability of MCF-7 cells treated with the indicated concentrations of Que for 24 and 48 h, as determined by the Cell Cycle Kit-8 assay. (B) mRNA expression levels of ABCB1, <t>ABCC2</t> and ABCG2 following Que treatment. (C) Representative western blotting images, (D) protein expression levels of ABCB1, ABCC2 and ABCG2, and (E) Bax, Bcl-2, and cleaved caspase-3 were assessed by western blotting. (F) Representative images of flow cytometry and (G) apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. Data are presented as the mean ± SEM of at least three independent experiments. Statistical significance was determined by one/two-way ANOVA followed by Bonferroni's post hoc test for multiple comparisons. *P<0.05, **P<0.01 compared with the control group, ## P<0.01 compared with the Que (24 h) group. Que, Quercetin; Ctrl, control.

Abcc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abcc2/MRP2%2FABCC2+Rabbit+mAb/pmc12891929-46-44-56
Average 94 stars, based on 21 article reviews

abcc2 - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells"

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells

Journal: Oncology Reports

doi: 10.3892/or.2026.9068

Figure Legend Snippet: Effects of Que on cell viability, mRNA levels, apoptosis and protein expression in MCF-7 cells. (A) Cell viability of MCF-7 cells treated with the indicated concentrations of Que for 24 and 48 h, as determined by the Cell Cycle Kit-8 assay. (B) mRNA expression levels of ABCB1, ABCC2 and ABCG2 following Que treatment. (C) Representative western blotting images, (D) protein expression levels of ABCB1, ABCC2 and ABCG2, and (E) Bax, Bcl-2, and cleaved caspase-3 were assessed by western blotting. (F) Representative images of flow cytometry and (G) apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. Data are presented as the mean ± SEM of at least three independent experiments. Statistical significance was determined by one/two-way ANOVA followed by Bonferroni's post hoc test for multiple comparisons. *P<0.05, **P<0.01 compared with the control group, ## P<0.01 compared with the Que (24 h) group. Que, Quercetin; Ctrl, control.

Techniques Used: Expressing, Western Blot, Flow Cytometry, Staining, Control

Effects of PTEN overexpression in MCF-7 cells. mRNA expression levels of (A) PTEN, (B) ABCB1, ABCC2, ABCG2, Bax and Bcl-2 following PTEN overexpression. (C) Representative western blotting images and protein expression levels of (D) ABCB1, ABCC2, ABCG2, (E) PTEN, Bax and Bcl-2 following PTEN overexpression. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the Plvx-con group.

Figure Legend Snippet: Effects of PTEN overexpression in MCF-7 cells. mRNA expression levels of (A) PTEN, (B) ABCB1, ABCC2, ABCG2, Bax and Bcl-2 following PTEN overexpression. (C) Representative western blotting images and protein expression levels of (D) ABCB1, ABCC2, ABCG2, (E) PTEN, Bax and Bcl-2 following PTEN overexpression. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the Plvx-con group.

Techniques Used: Over Expression, Expressing, Western Blot

Effects of combined PTEN overexpression and Que treatment on ABC transporters and the PI3K/AKT signaling pathway in MCF-7 cells. mRNA expression levels of (A) ABCB1, (B) ABCC2 (C) and ABCG2 in different treatment groups. (D) Representative images and (E) protein expression levels of ABCB1, ABCC2, ABCG2, (F) p-PI3K, and (G) p-AKT were evaluated by western blotting. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01, ***P<0.001 compared with the Plvx-con group. Que, Quercetin; con, control; p, phosphorylated.

Figure Legend Snippet: Effects of combined PTEN overexpression and Que treatment on ABC transporters and the PI3K/AKT signaling pathway in MCF-7 cells. mRNA expression levels of (A) ABCB1, (B) ABCC2 (C) and ABCG2 in different treatment groups. (D) Representative images and (E) protein expression levels of ABCB1, ABCC2, ABCG2, (F) p-PI3K, and (G) p-AKT were evaluated by western blotting. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01, ***P<0.001 compared with the Plvx-con group. Que, Quercetin; con, control; p, phosphorylated.

Techniques Used: Over Expression, Expressing, Western Blot, Control

Effects of combined LY294002 and Que treatment on apoptosis and protein expression in MCF-7 cells. (A) Representative western blotting images and Protein expression levels of (B) ABCB1, ABCC2, ABCG2, (C) p-PI3K (D) PTEN, Bax, Bcl-2 and (E) p-AKT in different treatment groups. (F) mRNA expression levels of ABCB1, ABCC2, ABCG2, PTEN, Bax and Bcl-2 in different treatment groups. (G) Representative immunofluorescence images of ABCG2 expression (green). Nuclei were counterstained with DAPI (blue). Scale bar, 50 µm. (H) Representative flow cytometry images and (I) Apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using two-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the control group; ## P<0.05 compared with the Que group. Que, Quercetin; Ctrl, control; p, phosphorylated.

Figure Legend Snippet: Effects of combined LY294002 and Que treatment on apoptosis and protein expression in MCF-7 cells. (A) Representative western blotting images and Protein expression levels of (B) ABCB1, ABCC2, ABCG2, (C) p-PI3K (D) PTEN, Bax, Bcl-2 and (E) p-AKT in different treatment groups. (F) mRNA expression levels of ABCB1, ABCC2, ABCG2, PTEN, Bax and Bcl-2 in different treatment groups. (G) Representative immunofluorescence images of ABCG2 expression (green). Nuclei were counterstained with DAPI (blue). Scale bar, 50 µm. (H) Representative flow cytometry images and (I) Apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using two-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the control group; ## P<0.05 compared with the Que group. Que, Quercetin; Ctrl, control; p, phosphorylated.

Techniques Used: Expressing, Western Blot, Immunofluorescence, Flow Cytometry, Staining, Control

Control:

Article Title: The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol
Article Snippet: .. Several proteins were analyzed, including: ABCB1 (cat. no. 13342), ABCC2 (cat. no. 4446) and ABCG2 (cat. no. 4477; all Cell Signaling Technology, Inc.) for drug efflux, deoxycytidine kinase (DCK; cat. no. GTX632065), thymidine kinase (TK)1 (cat. no. GTX113281; both GeneTex, Inc) and TK2 (cat. no. ab123785; Abcam) for drug metabolism, and poly (ADP-ribose) polymerase (PARP; cat. no. 9542; Cell Signaling Technology, Inc.) cleavage for apoptosis. a-tubulin (cat. no. MS-581) was used as a loading control. ..

Article Title: Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo .
Article Snippet: .. To determine GH induced and activated (phosphorylated) intracellular signaling molecules, protein extracts from FlucB16-F10 and Hepa1-6 cells and tumors were assayed for STAT5, AKT, ERK1/2, SRC; ABCB1, ABCG2, ABCC1, ABCC2, and ABCC4 (#9351, #4058, #4370, #2101, #94205, #4685, #9102, #2109, #13342, #42078, #72202, #12559, #12857, CST, Danvers, MA, USA); ABCB8 and ABCC9 (#PA5-76139, # PA5-42398, Thermo Fisher Scientific, Waltham, MA, USA); and ABCG1 (NB400-132, Novus Biologicals, Littleton, CO, USA) as previously described (29). b-actin (#4970, CST, Danvers, MA, USA) was used as a loading control. .. Anti-rabbit IgG, HRPlinked secondary antibody (#7074, CST, Danvers, MA, USA) and SuperSignal West Femto Maximum Sensitivity Substrate (#34095, Thermo Fisher Scientific, Waltham, MA, USA) were used.

Bioprocessing:

Article Title: Measurement of tumor antioxidant capacity and prediction of chemotherapy resistance in preclinical models of ovarian cancer by positron emission tomography
Article Snippet: .. Rabbit monoclonal antibodies against human xCT (Novus Biologicals; NB300-318), NRF2 (Cell Signaling Technology; 12721), GLS1 (Abcam; ab156876), GCL (Abcam; ab190685; 1:1000 dilution), ABCC2 (Cell Signaling Technology; 12559), ABCC6 (Cell Signaling Technology; 10666), ABCB1 (Cell Signaling Technology; 13342) and ABCG2 (Cell Signaling Technology; 42078) were used for cell lysate analysis. .. Rabbit anti-human NRF2, GCL, p53 (Cell Signaling Technology; 2527), caspase3 Cell Signaling Technology; 9915) and cleaved-caspase3 antibodies (Cell Signaling Technology; 9915; 1:1000 dilution) were used for tumor lysate evaluation (prepared as described below).

Incubation:

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells
Article Snippet: .. Primary antibodies included PTEN (1:5,000; cat. no. ab267787), Bcl-2 (1:6,000; cat. no. ab196495), Bax (1:6,000; cat. no. ab32503) and ABCG2 (1:5,000; cat. no. ab108312) from Abcam; p-PI3K (p85) (1:2,000; cat. no. 4228), p-AKT (Ser473) (1:2,000; cat. no. 4060), cleaved caspase-3 (1:5,000; cat. no. 9664), ABCC2 (1:1,000; cat. no. 12559) and ABCB1 (1:2,000; cat. no. 13342) from Cell Signaling Technology, Inc.; and GAPDH (1:12,000; cat. no. 60004-1-Ig), PI3K (1:3,000; cat. no. 20584-1-AP), AKT (1:3,000; 10176-2-AP), Caspase-3 (1:3,000; 19677-1-AP) and β-actin (1:10,000; cat. no. 66009-1-Ig) from Proteintech Group, Inc. After incubation with HRP-conjugated secondary antibodies (1:10,000; cat. no. SA00001-1 and SA00001-2; Proteintech Group, Inc.), protein bands were visualized using an ECL reagent (Beijing Lanbolide Trading Co., Ltd.) and imaged with a ChemiDoc XRS+ system (Bio-Rad Laboratories, Inc.). .. Total RNA was extracted from MCF-7 cells using TRIzol ® (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

other:

Article Title: Upregulation of Chemoresistance by Mg 2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells
Article Snippet: ABCC2 , 4446S , 1 , Cell Signaling Technology , Danvers, MA, USA.

Image Search Results

Journal: Oncology Reports

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells

doi: 10.3892/or.2026.9068

Figure Lengend Snippet: Effects of Que on cell viability, mRNA levels, apoptosis and protein expression in MCF-7 cells. (A) Cell viability of MCF-7 cells treated with the indicated concentrations of Que for 24 and 48 h, as determined by the Cell Cycle Kit-8 assay. (B) mRNA expression levels of ABCB1, ABCC2 and ABCG2 following Que treatment. (C) Representative western blotting images, (D) protein expression levels of ABCB1, ABCC2 and ABCG2, and (E) Bax, Bcl-2, and cleaved caspase-3 were assessed by western blotting. (F) Representative images of flow cytometry and (G) apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. Data are presented as the mean ± SEM of at least three independent experiments. Statistical significance was determined by one/two-way ANOVA followed by Bonferroni's post hoc test for multiple comparisons. *P<0.05, **P<0.01 compared with the control group, ## P<0.01 compared with the Que (24 h) group. Que, Quercetin; Ctrl, control.

Article Snippet: Primary antibodies included PTEN (1:5,000; cat. no. ab267787), Bcl-2 (1:6,000; cat. no. ab196495), Bax (1:6,000; cat. no. ab32503) and ABCG2 (1:5,000; cat. no. ab108312) from Abcam; p-PI3K (p85) (1:2,000; cat. no. 4228), p-AKT (Ser473) (1:2,000; cat. no. 4060), cleaved caspase-3 (1:5,000; cat. no. 9664), ABCC2 (1:1,000; cat. no. 12559) and ABCB1 (1:2,000; cat. no. 13342) from Cell Signaling Technology, Inc.; and GAPDH (1:12,000; cat. no. 60004-1-Ig), PI3K (1:3,000; cat. no. 20584-1-AP), AKT (1:3,000; 10176-2-AP), Caspase-3 (1:3,000; 19677-1-AP) and β-actin (1:10,000; cat. no. 66009-1-Ig) from Proteintech Group, Inc. After incubation with HRP-conjugated secondary antibodies (1:10,000; cat. no. SA00001-1 and SA00001-2; Proteintech Group, Inc.), protein bands were visualized using an ECL reagent (Beijing Lanbolide Trading Co., Ltd.) and imaged with a ChemiDoc XRS+ system (Bio-Rad Laboratories, Inc.).

Techniques: Expressing, Western Blot, Flow Cytometry, Staining, Control

Journal: Oncology Reports

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells

doi: 10.3892/or.2026.9068

Figure Lengend Snippet: Effects of PTEN overexpression in MCF-7 cells. mRNA expression levels of (A) PTEN, (B) ABCB1, ABCC2, ABCG2, Bax and Bcl-2 following PTEN overexpression. (C) Representative western blotting images and protein expression levels of (D) ABCB1, ABCC2, ABCG2, (E) PTEN, Bax and Bcl-2 following PTEN overexpression. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the Plvx-con group.

Techniques: Over Expression, Expressing, Western Blot

Journal: Oncology Reports

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells

doi: 10.3892/or.2026.9068

Figure Lengend Snippet: Effects of combined PTEN overexpression and Que treatment on ABC transporters and the PI3K/AKT signaling pathway in MCF-7 cells. mRNA expression levels of (A) ABCB1, (B) ABCC2 (C) and ABCG2 in different treatment groups. (D) Representative images and (E) protein expression levels of ABCB1, ABCC2, ABCG2, (F) p-PI3K, and (G) p-AKT were evaluated by western blotting. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using one-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01, ***P<0.001 compared with the Plvx-con group. Que, Quercetin; con, control; p, phosphorylated.

Techniques: Over Expression, Expressing, Western Blot, Control

Journal: Oncology Reports

Article Title: Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells

doi: 10.3892/or.2026.9068

Figure Lengend Snippet: Effects of combined LY294002 and Que treatment on apoptosis and protein expression in MCF-7 cells. (A) Representative western blotting images and Protein expression levels of (B) ABCB1, ABCC2, ABCG2, (C) p-PI3K (D) PTEN, Bax, Bcl-2 and (E) p-AKT in different treatment groups. (F) mRNA expression levels of ABCB1, ABCC2, ABCG2, PTEN, Bax and Bcl-2 in different treatment groups. (G) Representative immunofluorescence images of ABCG2 expression (green). Nuclei were counterstained with DAPI (blue). Scale bar, 50 µm. (H) Representative flow cytometry images and (I) Apoptosis rate was analyzed by flow cytometry after Annexin V-FITC/PI staining. The results are presented as the mean ± SEM of at least three independent experiments and analyzed using two-way ANOVA followed by Bonferroni's post hoc test. *P<0.05, **P<0.01 compared with the control group; ## P<0.05 compared with the Que group. Que, Quercetin; Ctrl, control; p, phosphorylated.

Techniques: Expressing, Western Blot, Immunofluorescence, Flow Cytometry, Staining, Control

Effects of Immune Education, CLP, and Specific Cytokine Blockade on CLP-Induced Hepatic Dysfunction. Immune Educated (Red) or Control (Blue) C57Bl/6 mice were sacrificed prior to or 24 h. following CLP alone or with cytokine blockade. A Hepatic tissue was assessed by RT-PCR for log 10 transformed relative transcription levels of SCLO1a1, SLC10, and ABCC2. B Serum Alanine aminotransferase (ALT). C Log 10 transformed peritoneal bacterial colony forming units. D Probability of Survival in the days following CLP with blockade as noted. Data as mean ± SD. Analyzed by 2-way ANOVA. * = p < 0.05 for comparisons between two groups. P Int = Significant Interaction between Blockade and Education, P Block = Significant Effect of Treatment (CLP + antibody blockade), P Edu = Significant Effect of Control vs Immune Education. For A , primary 2-way ANOVA comparison between Baseline, CLP, CLP + αIFNγ, CLP + αIL12p40, CLP + αTNF, and CLP + αIL17A/F. IL17A and IL17F blockade compared to each other separately from other comparisons. Survival analyzed by Log-rank Test. N = 4–22/group

Journal: Molecular Medicine

Article Title: IL17F + naïve and IFNγ + memory CD8 T cells drive hepatic dysfunction in the cecal ligation and puncture model of sepsis

doi: 10.1186/s10020-025-01411-2

Figure Lengend Snippet: Effects of Immune Education, CLP, and Specific Cytokine Blockade on CLP-Induced Hepatic Dysfunction. Immune Educated (Red) or Control (Blue) C57Bl/6 mice were sacrificed prior to or 24 h. following CLP alone or with cytokine blockade. A Hepatic tissue was assessed by RT-PCR for log 10 transformed relative transcription levels of SCLO1a1, SLC10, and ABCC2. B Serum Alanine aminotransferase (ALT). C Log 10 transformed peritoneal bacterial colony forming units. D Probability of Survival in the days following CLP with blockade as noted. Data as mean ± SD. Analyzed by 2-way ANOVA. * = p < 0.05 for comparisons between two groups. P Int = Significant Interaction between Blockade and Education, P Block = Significant Effect of Treatment (CLP + antibody blockade), P Edu = Significant Effect of Control vs Immune Education. For A , primary 2-way ANOVA comparison between Baseline, CLP, CLP + αIFNγ, CLP + αIL12p40, CLP + αTNF, and CLP + αIL17A/F. IL17A and IL17F blockade compared to each other separately from other comparisons. Survival analyzed by Log-rank Test. N = 4–22/group

Article Snippet: Primers were obtained from Applied Biosystems: TaqMan Gene Expression Assays Mm00496899_m1 (ABCC2), Mm00441421_m1 (SLC10a1), Mm01267415_m1 (SLCO1a1), and Mm99999915_g1 (Gapdh).

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Transformation Assay, Blocking Assay, Comparison

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abcc2 (Cell Signaling Technology Inc)

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1) Product Images from "Quercetin reduces expression of ATP-binding cassette transporters by regulating the PTEN/PI3K/AKT signaling pathway in breast cancer cells"

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