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aavrg ef1a mcherry ires cre  (Addgene inc)


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    Structured Review

    Addgene inc aavrg ef1a mcherry ires cre
    Aavrg Ef1a Mcherry Ires Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aavrg/pAAV-Ef1a-mCherry-IRES-Cre+(Plasmid+%2355632)/pmc13621956-536-6-7
    Average 96 stars, based on 114 article reviews
    aavrg ef1a mcherry ires cre - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: Thalamofrontal synaptic weakening underlies short-term memory deficits from adolescent NMDAR hypofunction
    Article Snippet: Viral or tracer injections were delivered through a Hamilton microsyringe (32–35 gauge tip) connected to an UltraMicroPump (WPI, USA) and controlled by a Micro4 controller (WPI, USA) at a rate of ∼50 nL/min. .. Mice were bilaterally injected with retroAAV-EF1a-Cre (2.5 × 1013 genome copies per mL (GC/mL), Addgene #55636-AAVrg) or retroAAV-EF1a-mCherry-IRES-Cre (7.0 × 1012 GC/mL, Addgene #55632-AAVrg) into the dmPFC layer 2/3 (300 nL per site), and with AAV2-hSyn-DIO-hM3Dq-mCherry (2.0 × 1013 /mL, packaged by VIUS from Addgene plasmid #44361) into the MD (300 nL per side). .. AAV1/2-hSyn-oChIEF-mCherry-WPRE (1.0 × 1013 GC/mL, Sirion Biotech GmbH #KA16140_AA3929) was also injected bilaterally into the MD (200 nL per side), either alone or in combination with hM3Dq for electrophysiological experiments.

    Article Title: An amygdaloreticular pathway promotes motor programs of ingestion.
    Article Snippet: .. Mice were placed on a heating pad (Stoelting, Rodent Warmer REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV5-hSynapsin1-FLEX-axon-GCaMP6s Broussard et al.46 Addgene Cat# 112010-AAV5; RRID: Addgene_112010 AAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] Klapoetke et al.47 Addgene Cat# 62723-AAV5; RRID: Addgene_62723 AAV1-EF1a-DIO-PdCO-mScarlet-ERminiWPRE Wietek et al.48 Addgene Cat# 198516-AAV1; RRID: Addgene_198516 AAV8-EF1a-DIO-SynaptophysinmCherry Virovek Custom prep AAVrg-hSyn-Cre-WPRE-hGH Addgene Addgene Cat# 105553-AAVrg; RRID: Addgene_105553 AAV-CAG-NLS-GFP Challis et al.49 Addgene Cat# 104061-PHPeB; RRID: Addgene_104061 Experimental models: Organisms/strains Mouse: Vgat-ires-cre knock-in; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:028862 Software and algorithms MATLAB R2023a Mathworks http://www.mathworks.com/products/ MATLAB/; RRID: SCR_001622 GraphPad Prism 10.4.1 GraphPad http://www.graphpad.com/; RRID:SCR_002798 DeepLabCut Mathis et al.50 deeplabcut.org; RRID:SCR_021391 Simple Behavior Analysis (SimBA) University of Washington https://edspace.american.edu/ openbehavior/project/simba/; Goodwin et al.51; RRID:SCR_021411 Fiji NIH http://fiji.sc; Schindelin et al.5; RRID:SCR_002285 Ethovision; Version 14 https://www.noldus.com/ethovision; RRID:SCR_000441 Custom MATLAB analysis code This study Zenodo: https://doi.org/10.5281/zenodo. ..

    Injection:

    Article Title: Thalamofrontal synaptic weakening underlies short-term memory deficits from adolescent NMDAR hypofunction
    Article Snippet: Viral or tracer injections were delivered through a Hamilton microsyringe (32–35 gauge tip) connected to an UltraMicroPump (WPI, USA) and controlled by a Micro4 controller (WPI, USA) at a rate of ∼50 nL/min. .. Mice were bilaterally injected with retroAAV-EF1a-Cre (2.5 × 1013 genome copies per mL (GC/mL), Addgene #55636-AAVrg) or retroAAV-EF1a-mCherry-IRES-Cre (7.0 × 1012 GC/mL, Addgene #55632-AAVrg) into the dmPFC layer 2/3 (300 nL per site), and with AAV2-hSyn-DIO-hM3Dq-mCherry (2.0 × 1013 /mL, packaged by VIUS from Addgene plasmid #44361) into the MD (300 nL per side). .. AAV1/2-hSyn-oChIEF-mCherry-WPRE (1.0 × 1013 GC/mL, Sirion Biotech GmbH #KA16140_AA3929) was also injected bilaterally into the MD (200 nL per side), either alone or in combination with hM3Dq for electrophysiological experiments.

    Plasmid Preparation:

    Article Title: Thalamofrontal synaptic weakening underlies short-term memory deficits from adolescent NMDAR hypofunction
    Article Snippet: Viral or tracer injections were delivered through a Hamilton microsyringe (32–35 gauge tip) connected to an UltraMicroPump (WPI, USA) and controlled by a Micro4 controller (WPI, USA) at a rate of ∼50 nL/min. .. Mice were bilaterally injected with retroAAV-EF1a-Cre (2.5 × 1013 genome copies per mL (GC/mL), Addgene #55636-AAVrg) or retroAAV-EF1a-mCherry-IRES-Cre (7.0 × 1012 GC/mL, Addgene #55632-AAVrg) into the dmPFC layer 2/3 (300 nL per site), and with AAV2-hSyn-DIO-hM3Dq-mCherry (2.0 × 1013 /mL, packaged by VIUS from Addgene plasmid #44361) into the MD (300 nL per side). .. AAV1/2-hSyn-oChIEF-mCherry-WPRE (1.0 × 1013 GC/mL, Sirion Biotech GmbH #KA16140_AA3929) was also injected bilaterally into the MD (200 nL per side), either alone or in combination with hM3Dq for electrophysiological experiments.

    other:

    Article Title: Gut-to-brain signaling restricts dietary protein intake during recovery from catabolic states
    Article Snippet: Ppara −/− : B6;129S4- Pparatm1Gonz /J , The Jackson Laboratory , #008154;RRID:IMSR_JAX:008154.

    Virus:

    Article Title: Locus coeruleus microcircuitry processes periaqueductal gray inputs into distinct outputs for regulation of pain and anxiety.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-c-Fos antibody Abcam Cat#ab190289; RRID: AB_2737414 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, 488 Invitrogen Cat# A-21206; RRID:AB_2535792 Sheep Anti-Tyrosine Hydroxylase Antibody, polyclonal EMD Millipore Cat#AB1542; RRID:AB_90755 Donkey anti-sheep IgG (H + L) Secondary Antibody, 647 Invitrogen Cat#A21448; RRID: AB_2535865 Mouse Anti-Noradrenaline transporter antibody Abcam Cat#ab211463; RRID:AB_2891028 Donkey anti-mouse IgG (H + L) Secondary Antibody, 594 Invitrogen Cat#A21203; RRID: AB_2535789 Donkey anti-mouse IgG (H + L) Secondary Antibody, 647 Invitrogen Cat#A31571; RRID: AB_162542 Bacterial and virus strains AAV9-EF1α-double floxed-ChR2 (H134R)EYFP-WPRE-HGHpA Addgene #20298-AAV9; RRID:Addgene_20298 AAV1-CAG-flexFRT-ChR2(H134R)mCherry Addgene #75470-AAV1; RRID:Addgene_75470 AAVrg-EF1a-DIO-FLPo-WPRE-hGHpA Addgene #87306-AAVrg; RRID:Addgene_87306 AAV9-EF1a-DIO-C1V1(E122T/E162t)TS-eYFP Addgene #35497-AAV9; RRID:Addgene_35497 AAVrg-syn-jGCaMP7s-WPRE Addgene #104487-AAVrg; RRID:Addgene_104487 AAV5-EF1a-mCherry-Flex-dtA UNC GTC vector lot#AV6240B AAV9-EF1a-DIO-EYFP, Addgene #27056-AAV9, Addgene #27056-AAV9; RRID:Addgene_27056 AAVrg-pgk-Cre Addgene #24593-AAVrg, Addgene #24593-AAVrg; RRID:Addgene_24593 AAV9-CaMKIIa-ChR2(H134R)-mCherry, Addgene #26975-AAV9, Addgene #26975-AAV9; RRID:Addgene_26975 AAV5-CaMKIIalpha-ChR2-eYFP UNC GTC Vector Core lot# NV4316N AAV5-EF1a-DIO-eNpHR3.0-eYFP UNC GTC Vector Core Lot#AV4806G Chemicals, peptides, and recombinant proteins Alexa Fluor 488 conjugate Thermo Fisher Cat#C22841 TTX Citrate Alomone Cat#T-550 4-AP (4-amnopyrindine) Millipore Sigma Cat#A-0152 Bicuculline methochloride Tocris Cat#0131/10 DNQX Tocris Cat#0189/10 QX314 chloride Tocris Cat#2313/50 DSP-4 Millipore Sigma Cat#C8417 Experimental models: organisms/strains Vglut2-ires-Cre mice: Strain #:016963 The Jackson lab RRID:IMSR_JAX:016963 Vgat-ires-Cre mice: Strain #:028862 The Jackson lab RRID:IMSR_JAX:02886 Ai9 or Ai9(RCL-tdT): Strain #:007909 The Jackson lab RRID:IMSR_JAX:007909 (Continued on next page) Cell Reports 44, 116699, December 23, 2025 19 ..

    Article Title: An amygdaloreticular pathway promotes motor programs of ingestion.
    Article Snippet: .. Mice were placed on a heating pad (Stoelting, Rodent Warmer REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV5-hSynapsin1-FLEX-axon-GCaMP6s Broussard et al.46 Addgene Cat# 112010-AAV5; RRID: Addgene_112010 AAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] Klapoetke et al.47 Addgene Cat# 62723-AAV5; RRID: Addgene_62723 AAV1-EF1a-DIO-PdCO-mScarlet-ERminiWPRE Wietek et al.48 Addgene Cat# 198516-AAV1; RRID: Addgene_198516 AAV8-EF1a-DIO-SynaptophysinmCherry Virovek Custom prep AAVrg-hSyn-Cre-WPRE-hGH Addgene Addgene Cat# 105553-AAVrg; RRID: Addgene_105553 AAV-CAG-NLS-GFP Challis et al.49 Addgene Cat# 104061-PHPeB; RRID: Addgene_104061 Experimental models: Organisms/strains Mouse: Vgat-ires-cre knock-in; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:028862 Software and algorithms MATLAB R2023a Mathworks http://www.mathworks.com/products/ MATLAB/; RRID: SCR_001622 GraphPad Prism 10.4.1 GraphPad http://www.graphpad.com/; RRID:SCR_002798 DeepLabCut Mathis et al.50 deeplabcut.org; RRID:SCR_021391 Simple Behavior Analysis (SimBA) University of Washington https://edspace.american.edu/ openbehavior/project/simba/; Goodwin et al.51; RRID:SCR_021411 Fiji NIH http://fiji.sc; Schindelin et al.5; RRID:SCR_002285 Ethovision; Version 14 https://www.noldus.com/ethovision; RRID:SCR_000441 Custom MATLAB analysis code This study Zenodo: https://doi.org/10.5281/zenodo. ..

    Recombinant:

    Article Title: Locus coeruleus microcircuitry processes periaqueductal gray inputs into distinct outputs for regulation of pain and anxiety.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-c-Fos antibody Abcam Cat#ab190289; RRID: AB_2737414 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, 488 Invitrogen Cat# A-21206; RRID:AB_2535792 Sheep Anti-Tyrosine Hydroxylase Antibody, polyclonal EMD Millipore Cat#AB1542; RRID:AB_90755 Donkey anti-sheep IgG (H + L) Secondary Antibody, 647 Invitrogen Cat#A21448; RRID: AB_2535865 Mouse Anti-Noradrenaline transporter antibody Abcam Cat#ab211463; RRID:AB_2891028 Donkey anti-mouse IgG (H + L) Secondary Antibody, 594 Invitrogen Cat#A21203; RRID: AB_2535789 Donkey anti-mouse IgG (H + L) Secondary Antibody, 647 Invitrogen Cat#A31571; RRID: AB_162542 Bacterial and virus strains AAV9-EF1α-double floxed-ChR2 (H134R)EYFP-WPRE-HGHpA Addgene #20298-AAV9; RRID:Addgene_20298 AAV1-CAG-flexFRT-ChR2(H134R)mCherry Addgene #75470-AAV1; RRID:Addgene_75470 AAVrg-EF1a-DIO-FLPo-WPRE-hGHpA Addgene #87306-AAVrg; RRID:Addgene_87306 AAV9-EF1a-DIO-C1V1(E122T/E162t)TS-eYFP Addgene #35497-AAV9; RRID:Addgene_35497 AAVrg-syn-jGCaMP7s-WPRE Addgene #104487-AAVrg; RRID:Addgene_104487 AAV5-EF1a-mCherry-Flex-dtA UNC GTC vector lot#AV6240B AAV9-EF1a-DIO-EYFP, Addgene #27056-AAV9, Addgene #27056-AAV9; RRID:Addgene_27056 AAVrg-pgk-Cre Addgene #24593-AAVrg, Addgene #24593-AAVrg; RRID:Addgene_24593 AAV9-CaMKIIa-ChR2(H134R)-mCherry, Addgene #26975-AAV9, Addgene #26975-AAV9; RRID:Addgene_26975 AAV5-CaMKIIalpha-ChR2-eYFP UNC GTC Vector Core lot# NV4316N AAV5-EF1a-DIO-eNpHR3.0-eYFP UNC GTC Vector Core Lot#AV4806G Chemicals, peptides, and recombinant proteins Alexa Fluor 488 conjugate Thermo Fisher Cat#C22841 TTX Citrate Alomone Cat#T-550 4-AP (4-amnopyrindine) Millipore Sigma Cat#A-0152 Bicuculline methochloride Tocris Cat#0131/10 DNQX Tocris Cat#0189/10 QX314 chloride Tocris Cat#2313/50 DSP-4 Millipore Sigma Cat#C8417 Experimental models: organisms/strains Vglut2-ires-Cre mice: Strain #:016963 The Jackson lab RRID:IMSR_JAX:016963 Vgat-ires-Cre mice: Strain #:028862 The Jackson lab RRID:IMSR_JAX:02886 Ai9 or Ai9(RCL-tdT): Strain #:007909 The Jackson lab RRID:IMSR_JAX:007909 (Continued on next page) Cell Reports 44, 116699, December 23, 2025 19 ..

    Bioprocessing:

    Article Title: An amygdaloreticular pathway promotes motor programs of ingestion.
    Article Snippet: .. Mice were placed on a heating pad (Stoelting, Rodent Warmer REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV5-hSynapsin1-FLEX-axon-GCaMP6s Broussard et al.46 Addgene Cat# 112010-AAV5; RRID: Addgene_112010 AAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] Klapoetke et al.47 Addgene Cat# 62723-AAV5; RRID: Addgene_62723 AAV1-EF1a-DIO-PdCO-mScarlet-ERminiWPRE Wietek et al.48 Addgene Cat# 198516-AAV1; RRID: Addgene_198516 AAV8-EF1a-DIO-SynaptophysinmCherry Virovek Custom prep AAVrg-hSyn-Cre-WPRE-hGH Addgene Addgene Cat# 105553-AAVrg; RRID: Addgene_105553 AAV-CAG-NLS-GFP Challis et al.49 Addgene Cat# 104061-PHPeB; RRID: Addgene_104061 Experimental models: Organisms/strains Mouse: Vgat-ires-cre knock-in; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:028862 Software and algorithms MATLAB R2023a Mathworks http://www.mathworks.com/products/ MATLAB/; RRID: SCR_001622 GraphPad Prism 10.4.1 GraphPad http://www.graphpad.com/; RRID:SCR_002798 DeepLabCut Mathis et al.50 deeplabcut.org; RRID:SCR_021391 Simple Behavior Analysis (SimBA) University of Washington https://edspace.american.edu/ openbehavior/project/simba/; Goodwin et al.51; RRID:SCR_021411 Fiji NIH http://fiji.sc; Schindelin et al.5; RRID:SCR_002285 Ethovision; Version 14 https://www.noldus.com/ethovision; RRID:SCR_000441 Custom MATLAB analysis code This study Zenodo: https://doi.org/10.5281/zenodo. ..

    Knock-In:

    Article Title: An amygdaloreticular pathway promotes motor programs of ingestion.
    Article Snippet: .. Mice were placed on a heating pad (Stoelting, Rodent Warmer REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV5-hSynapsin1-FLEX-axon-GCaMP6s Broussard et al.46 Addgene Cat# 112010-AAV5; RRID: Addgene_112010 AAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] Klapoetke et al.47 Addgene Cat# 62723-AAV5; RRID: Addgene_62723 AAV1-EF1a-DIO-PdCO-mScarlet-ERminiWPRE Wietek et al.48 Addgene Cat# 198516-AAV1; RRID: Addgene_198516 AAV8-EF1a-DIO-SynaptophysinmCherry Virovek Custom prep AAVrg-hSyn-Cre-WPRE-hGH Addgene Addgene Cat# 105553-AAVrg; RRID: Addgene_105553 AAV-CAG-NLS-GFP Challis et al.49 Addgene Cat# 104061-PHPeB; RRID: Addgene_104061 Experimental models: Organisms/strains Mouse: Vgat-ires-cre knock-in; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:028862 Software and algorithms MATLAB R2023a Mathworks http://www.mathworks.com/products/ MATLAB/; RRID: SCR_001622 GraphPad Prism 10.4.1 GraphPad http://www.graphpad.com/; RRID:SCR_002798 DeepLabCut Mathis et al.50 deeplabcut.org; RRID:SCR_021391 Simple Behavior Analysis (SimBA) University of Washington https://edspace.american.edu/ openbehavior/project/simba/; Goodwin et al.51; RRID:SCR_021411 Fiji NIH http://fiji.sc; Schindelin et al.5; RRID:SCR_002285 Ethovision; Version 14 https://www.noldus.com/ethovision; RRID:SCR_000441 Custom MATLAB analysis code This study Zenodo: https://doi.org/10.5281/zenodo. ..

    Analysis:

    Article Title: An amygdaloreticular pathway promotes motor programs of ingestion.
    Article Snippet: .. Mice were placed on a heating pad (Stoelting, Rodent Warmer REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV5-hSynapsin1-FLEX-axon-GCaMP6s Broussard et al.46 Addgene Cat# 112010-AAV5; RRID: Addgene_112010 AAV5-Syn-FLEX-rc[ChrimsonR-tdTomato] Klapoetke et al.47 Addgene Cat# 62723-AAV5; RRID: Addgene_62723 AAV1-EF1a-DIO-PdCO-mScarlet-ERminiWPRE Wietek et al.48 Addgene Cat# 198516-AAV1; RRID: Addgene_198516 AAV8-EF1a-DIO-SynaptophysinmCherry Virovek Custom prep AAVrg-hSyn-Cre-WPRE-hGH Addgene Addgene Cat# 105553-AAVrg; RRID: Addgene_105553 AAV-CAG-NLS-GFP Challis et al.49 Addgene Cat# 104061-PHPeB; RRID: Addgene_104061 Experimental models: Organisms/strains Mouse: Vgat-ires-cre knock-in; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:028862 Software and algorithms MATLAB R2023a Mathworks http://www.mathworks.com/products/ MATLAB/; RRID: SCR_001622 GraphPad Prism 10.4.1 GraphPad http://www.graphpad.com/; RRID:SCR_002798 DeepLabCut Mathis et al.50 deeplabcut.org; RRID:SCR_021391 Simple Behavior Analysis (SimBA) University of Washington https://edspace.american.edu/ openbehavior/project/simba/; Goodwin et al.51; RRID:SCR_021411 Fiji NIH http://fiji.sc; Schindelin et al.5; RRID:SCR_002285 Ethovision; Version 14 https://www.noldus.com/ethovision; RRID:SCR_000441 Custom MATLAB analysis code This study Zenodo: https://doi.org/10.5281/zenodo. ..



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