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aav packaging plasmid pucmini icap php eb  (Addgene inc)


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    Structured Review

    Addgene inc aav packaging plasmid pucmini icap php eb
    Aav Packaging Plasmid Pucmini Icap Php Eb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+packaging/pUCmini-iCAP-PHP%2EeB+(Plasmid+%23103005)/bio_rxiv__64898__2026__01__26__701845-289-1-8
    Average 97 stars, based on 201 article reviews
    aav packaging plasmid pucmini icap php eb - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Construct:

    Article Title: Microglia contribute to polyG-dependent neurodegeneration in neuronal intranuclear inclusion disease.
    Article Snippet: Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder caused by the expansion of GGC trinucleotide repeats in NOTCH2NLC gene.. Despite identifying uN2CpolyG, a toxic polyglycine (polyG) protein translated by expanded GGC repeats, the exact pathogenic mechanisms of NIID remain unclear.. In this study, we investigated the role of polyG by expressing various forms of NOTCH2NLC in mice: the wild-type, the expanded form with 100 GGC repeats (either translating or not translating into uN2CpolyG), and the mutated form that encodes a pure polyG without GGC-repeat RNA and the C-terminal stretch (uN2CpolyG-dCT).

    Bioprocessing:

    Article Title: Microglia contribute to polyG-dependent neurodegeneration in neuronal intranuclear inclusion disease.
    Article Snippet: Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder caused by the expansion of GGC trinucleotide repeats in NOTCH2NLC gene.. Despite identifying uN2CpolyG, a toxic polyglycine (polyG) protein translated by expanded GGC repeats, the exact pathogenic mechanisms of NIID remain unclear.. In this study, we investigated the role of polyG by expressing various forms of NOTCH2NLC in mice: the wild-type, the expanded form with 100 GGC repeats (either translating or not translating into uN2CpolyG), and the mutated form that encodes a pure polyG without GGC-repeat RNA and the C-terminal stretch (uN2CpolyG-dCT).

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52-54 deletion mice by CRISPR-Cas9-mediated exon skipping.
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAVCMV::NLS-SaCas9-NLS-3 HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Article Title: Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52–54 deletion mice by CRISPR-Cas9-mediated exon skipping
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAV-CMV::NLS-SaCas9-NLS-3×HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Article Title: Comparative cellular analysis of motor cortex in human, marmoset and mouse
    Article Snippet: The plasmid supplying the helper function is available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by V. Gradinaru at the California Institute of Technology , and the DNA plasmid for AAV packaging is available from Addgene (RRID Addgene_103005). ..

    Article Title: Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Molecular and genetic approaches for assaying human cell type synaptic connectivity
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Decreased CNNM2 expression in prefrontal cortex affects sensorimotor gating function, cognition, dendritic spine morphogenesis and risk of schizophrenia.
    Article Snippet: Genome-wide association studies (GWASs) have reported multiple single nucleotide polymorphisms (SNPs) associated with schizophrenia, yet the underlying molecular mechanisms are largely unknown.. In this study, we aimed to identify schizophrenia relevant genes showing alterations in mRNA and protein expression associated with risk SNPs at the 10q24.32-33 GWAS locus.. We carried out the quantitative trait loci (QTL) and summary data-based Mendelian randomization (SMR) analyses, using the PsychENCODE dorsolateral prefrontal cortex (DLPFC) expression QTL (eQTL) database, as well as the ROSMAP and Banner DLPFC protein QTL (pQTL) datasets.

    Clone Assay:

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52-54 deletion mice by CRISPR-Cas9-mediated exon skipping.
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAVCMV::NLS-SaCas9-NLS-3 HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52–54 deletion mice by CRISPR-Cas9-mediated exon skipping
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAV-CMV::NLS-SaCas9-NLS-3×HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Plasmid Preparation:

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52-54 deletion mice by CRISPR-Cas9-mediated exon skipping.
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAVCMV::NLS-SaCas9-NLS-3 HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Article Title: Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Prevention of early-onset cardiomyopathy in Dmd exon 52–54 deletion mice by CRISPR-Cas9-mediated exon skipping
    Article Snippet: .. The sgRNAs were cloned into an SaCas9-expressing plasmid containing inverted terminal repeats (ITRs) for AAV packaging (pX601-AAV-CMV::NLS-SaCas9-NLS-3×HA-bGHpA; U6::BsaI-sgRNA, Addgene plasmid 61591). .. Oligonucleotides were synthesized by Integrated DNA Technologies.

    Article Title: Comparative cellular analysis of motor cortex in human, marmoset and mouse
    Article Snippet: The plasmid supplying the helper function is available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by V. Gradinaru at the California Institute of Technology , and the DNA plasmid for AAV packaging is available from Addgene (RRID Addgene_103005). ..

    Article Title: Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Molecular and genetic approaches for assaying human cell type synaptic connectivity
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Decreased CNNM2 expression in prefrontal cortex affects sensorimotor gating function, cognition, dendritic spine morphogenesis and risk of schizophrenia.
    Article Snippet: Genome-wide association studies (GWASs) have reported multiple single nucleotide polymorphisms (SNPs) associated with schizophrenia, yet the underlying molecular mechanisms are largely unknown.. In this study, we aimed to identify schizophrenia relevant genes showing alterations in mRNA and protein expression associated with risk SNPs at the 10q24.32-33 GWAS locus.. We carried out the quantitative trait loci (QTL) and summary data-based Mendelian randomization (SMR) analyses, using the PsychENCODE dorsolateral prefrontal cortex (DLPFC) expression QTL (eQTL) database, as well as the ROSMAP and Banner DLPFC protein QTL (pQTL) datasets.

    Variant Assay:

    Article Title: Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Comparative cellular analysis of motor cortex in human, marmoset and mouse
    Article Snippet: The plasmid supplying the helper function is available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by V. Gradinaru at the California Institute of Technology , and the DNA plasmid for AAV packaging is available from Addgene (RRID Addgene_103005). ..

    Article Title: Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Molecular and genetic approaches for assaying human cell type synaptic connectivity
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Generated:

    Article Title: Target cell-specific synaptic dynamics of excitatory to inhibitory neuron connections in supragranular layers of human neocortex
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Comparative cellular analysis of motor cortex in human, marmoset and mouse
    Article Snippet: The plasmid supplying the helper function is available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by V. Gradinaru at the California Institute of Technology , and the DNA plasmid for AAV packaging is available from Addgene (RRID Addgene_103005). ..

    Article Title: Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Article Title: Molecular and genetic approaches for assaying human cell type synaptic connectivity
    Article Snippet: The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). .. The PHP.eB capsid variant was generated by Dr. Viviana Gradinaru at the California Institute of Technology ( ) and the DNA plasmid for AAV packaging is available from Addgene (plasmid#103005). ..

    Expressing:

    Article Title: Decreased CNNM2 expression in prefrontal cortex affects sensorimotor gating function, cognition, dendritic spine morphogenesis and risk of schizophrenia.
    Article Snippet: Genome-wide association studies (GWASs) have reported multiple single nucleotide polymorphisms (SNPs) associated with schizophrenia, yet the underlying molecular mechanisms are largely unknown.. In this study, we aimed to identify schizophrenia relevant genes showing alterations in mRNA and protein expression associated with risk SNPs at the 10q24.32-33 GWAS locus.. We carried out the quantitative trait loci (QTL) and summary data-based Mendelian randomization (SMR) analyses, using the PsychENCODE dorsolateral prefrontal cortex (DLPFC) expression QTL (eQTL) database, as well as the ROSMAP and Banner DLPFC protein QTL (pQTL) datasets.

    Control:

    Article Title: Decreased CNNM2 expression in prefrontal cortex affects sensorimotor gating function, cognition, dendritic spine morphogenesis and risk of schizophrenia.
    Article Snippet: Genome-wide association studies (GWASs) have reported multiple single nucleotide polymorphisms (SNPs) associated with schizophrenia, yet the underlying molecular mechanisms are largely unknown.. In this study, we aimed to identify schizophrenia relevant genes showing alterations in mRNA and protein expression associated with risk SNPs at the 10q24.32-33 GWAS locus.. We carried out the quantitative trait loci (QTL) and summary data-based Mendelian randomization (SMR) analyses, using the PsychENCODE dorsolateral prefrontal cortex (DLPFC) expression QTL (eQTL) database, as well as the ROSMAP and Banner DLPFC protein QTL (pQTL) datasets.



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    Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Journal: Frontiers in Immunology

    Article Title: Long-term protective potency of AAV vector-based SARS-CoV-2 prophylaxis in mice and non-human primates

    doi: 10.3389/fimmu.2026.1829230

    Figure Lengend Snippet: Analysis of serum P2C5-Fc concentration, neutralizing activity, and immunogenicity in mice following i.m. administration of rAAV-P2C5-Fc. (A) Study design. (B) Serum levels of the P2C5-Fc antibody following i.m. administration of rAAV-P2C5-Fc at a dose of a 1.0 × 10 13 gc/kg in mice. (C) NAb activity titers against SARS-CoV-2. (D) Antibody titers against AAV-DJ capsid proteins and the expressed P2C5-Fc antibody.

    Article Snippet: The rAAV-P2C5-Fc vector construct was produced using a three-plasmid AAV-DJ packaging system (Cell Biolabs, USA) by transient transfection of HEK293 cells (obtained from the cell collection of the N.F.

    Techniques: Concentration Assay, Activity Assay, Immunopeptidomics