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rabbit anti 8 ohdg  (Bioss)


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    Structured Review

    Bioss rabbit anti 8 ohdg
    Rabbit Anti 8 Ohdg, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/8+ohdg/8-OHdG+Polyclonal+Antibody/pm42089455-111-6-8
    Average 96 stars, based on 218 article reviews
    rabbit anti 8 ohdg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Nanocomposite-loaded hybrid microneedles reshape dermal microenvironment through senolytic delivery and collagen XVII-mediated barrier repair
    Article Snippet: .. Monoclonal antibodies including MMP1(10371-2-AP, Biotech) and MMP3(17873-1-AP, Biotech), 4-HNE(bs-6313R, Bioss), 8-OHdG(bs-1278R, Bioss), NRF2( GB113808 , Servicebio), HO-1(10701-1-AP, Proteintech), γH2A.X( GB111841 , servcebio), Vimentin (GB11192, servcebio), AQP3(GB11395, servicebio), CK-19(60187-1-AP, Proteintech), CK-5(GB111246-100, servicebio), CK-10 (GB112105-100, servicebio), were used for immunofluorescence and immunohistochemistry staining. .. Monoclonal antibodies against TGF-β (Abcam, ab92486), Collagen I (CST, cst91144s), Collagen III (Abcam, ab7778), and GAPDH (CST, cst2118) were used for protein detection.

    Immunofluorescence:

    Article Title: Nanocomposite-loaded hybrid microneedles reshape dermal microenvironment through senolytic delivery and collagen XVII-mediated barrier repair
    Article Snippet: .. Monoclonal antibodies including MMP1(10371-2-AP, Biotech) and MMP3(17873-1-AP, Biotech), 4-HNE(bs-6313R, Bioss), 8-OHdG(bs-1278R, Bioss), NRF2( GB113808 , Servicebio), HO-1(10701-1-AP, Proteintech), γH2A.X( GB111841 , servcebio), Vimentin (GB11192, servcebio), AQP3(GB11395, servicebio), CK-19(60187-1-AP, Proteintech), CK-5(GB111246-100, servicebio), CK-10 (GB112105-100, servicebio), were used for immunofluorescence and immunohistochemistry staining. .. Monoclonal antibodies against TGF-β (Abcam, ab92486), Collagen I (CST, cst91144s), Collagen III (Abcam, ab7778), and GAPDH (CST, cst2118) were used for protein detection.

    Article Title: Spatial multi-omics guides ASCT2-targeted delivery of glycolysis inhibitor for cervical cancer suppression and chemoresistance reversal.
    Article Snippet: .. Concurrently, tumor sections were subjected to immunofluorescence analysis of Ki-67 (1:200, Servicebio, GB151499), 4-HNE (1:400, Servicebio, GB150073), 8-OhdG (1:200, Bioss, bs-1278r), and MRP2 (1:200, Immunoway YT2840), with imaging performed on a ZEISS Apotome 3 system. .. Additionally, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) staining was conducted on tumor sections using a commercial kit (Servicebio, G1501) to detect apoptotic cells.

    Immunohistochemistry:

    Article Title: Nanocomposite-loaded hybrid microneedles reshape dermal microenvironment through senolytic delivery and collagen XVII-mediated barrier repair
    Article Snippet: .. Monoclonal antibodies including MMP1(10371-2-AP, Biotech) and MMP3(17873-1-AP, Biotech), 4-HNE(bs-6313R, Bioss), 8-OHdG(bs-1278R, Bioss), NRF2( GB113808 , Servicebio), HO-1(10701-1-AP, Proteintech), γH2A.X( GB111841 , servcebio), Vimentin (GB11192, servcebio), AQP3(GB11395, servicebio), CK-19(60187-1-AP, Proteintech), CK-5(GB111246-100, servicebio), CK-10 (GB112105-100, servicebio), were used for immunofluorescence and immunohistochemistry staining. .. Monoclonal antibodies against TGF-β (Abcam, ab92486), Collagen I (CST, cst91144s), Collagen III (Abcam, ab7778), and GAPDH (CST, cst2118) were used for protein detection.

    Staining:

    Article Title: Nanocomposite-loaded hybrid microneedles reshape dermal microenvironment through senolytic delivery and collagen XVII-mediated barrier repair
    Article Snippet: .. Monoclonal antibodies including MMP1(10371-2-AP, Biotech) and MMP3(17873-1-AP, Biotech), 4-HNE(bs-6313R, Bioss), 8-OHdG(bs-1278R, Bioss), NRF2( GB113808 , Servicebio), HO-1(10701-1-AP, Proteintech), γH2A.X( GB111841 , servcebio), Vimentin (GB11192, servcebio), AQP3(GB11395, servicebio), CK-19(60187-1-AP, Proteintech), CK-5(GB111246-100, servicebio), CK-10 (GB112105-100, servicebio), were used for immunofluorescence and immunohistochemistry staining. .. Monoclonal antibodies against TGF-β (Abcam, ab92486), Collagen I (CST, cst91144s), Collagen III (Abcam, ab7778), and GAPDH (CST, cst2118) were used for protein detection.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner.
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource (HIMSR) at the University of Colorado School of Medicine we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies HO-1 (Abcam [ab189491]; 1:1000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam [ab46545]; 1:100; pH6), 8-OHdG (BioSS [bs-1278R]; 1:300; pH9), Ki67 ([SP6] 1:400; pH6) and EPCAM (1:200; pH6), followed by horseradish peroxidase-conjugated secondary antibody polymer, and HRP-reactive OPAL fluorescent reagents. ..

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource at the University of Colorado School of Medicine, we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies, including HO-1 (Abcam, ab189491; 1:1,000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam, ab46545; 1:100; pH6), 8-OHdG (BioSS, bs-1278R; 1:300; pH9), Ki67 (SP6, 1:400; pH6), and EPCAM (1:200; pH6), followed by horseradish peroxidase–conjugated secondary antibody polymer and HRP-reactive OPAL fluorescent reagents. ..

    Incubation:

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner.
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource (HIMSR) at the University of Colorado School of Medicine we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies HO-1 (Abcam [ab189491]; 1:1000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam [ab46545]; 1:100; pH6), 8-OHdG (BioSS [bs-1278R]; 1:300; pH9), Ki67 ([SP6] 1:400; pH6) and EPCAM (1:200; pH6), followed by horseradish peroxidase-conjugated secondary antibody polymer, and HRP-reactive OPAL fluorescent reagents. ..

    Article Title: PKM2 preconditioning protects endothelial cells from pyroptosis and BBB disruption via NRF2/TRX/TXNIP signaling in neonatal hypoxic-ischemic brain injury.
    Article Snippet: .. For double-immunofluorescence labeling, the sections were incubated with antibodies against CD31 (R&D Systems Catalog #AF3628, 1:100), caspase-1 (Abcam; Catalog #ab179515, 1:200), ZO-1 (Abcam; Catalog #ab221547, 1:100), MMP2 (Affinity; Catalog #AF5228, 1:200), NRF2 (Proteintech; Catalog #16396-1- AP, 1:200), 8-OHdG (Bioss; Catalog #bs-1278R, 1:200) or PKM2 (ZENBIO; Catalog #381318, 1:100) overnight at 4 °C. .. Then, the sections were washed, treated with Alexa Fluor 488 or Alexa Fluor 594 secondary antibodies for 1 h, and incubated with 4,6-diamidino-2-phenylindole (DAPI) for 10 min. A Nikon fluorescence microscope was used to capture images.

    Article Title: PKM2 preconditioning protects endothelial cells from pyroptosis and BBB disruption via NRF2/TRX/TXNIP signaling in neonatal hypoxic-ischemic brain injury
    Article Snippet: .. For double-immunofluorescence labeling, the sections were incubated with antibodies against CD31 (R&D Systems Catalog #AF3628, 1:100), caspase-1 (Abcam; Catalog #ab179515, 1:200), ZO-1 (Abcam; Catalog #ab221547, 1:100), MMP2 (Affinity; Catalog #AF5228, 1:200), NRF2 (Proteintech; Catalog #16396-1-AP, 1:200), 8-OHdG (Bioss; Catalog #bs-1278R, 1:200) or PKM2 (ZENBIO; Catalog #381318, 1:100) overnight at 4 °C. .. Then, the sections were washed, treated with Alexa Fluor 488 or Alexa Fluor 594 secondary antibodies for 1 h, and incubated with 4,6-diamidino-2-phenylindole (DAPI) for 10 min. A Nikon fluorescence microscope was used to capture images.

    Article Title: Hypoxia-induced LONP1 overexpression and mtDNA damage may serve as biomarkers for death from mechanical asphyxia.
    Article Snippet: Due to the absence of reliable signs, the authentication of death from mechanical asphyxia (DMA), commonly seen in forensic pathology, can be rather challenging especially when the criminal case is obscure.. Lon protease-1 (LONP1) is an ATP-dependent serine protease located in mitochondria that participates in maintaining the integrity of the mitochondrial genome and regulating mitochondrial DNA (mtDNA) replication.. In this research, cerebral tissues of corpses with different causes of death were collected to compare the expression of LONP1 and the changes of mtDNA to see the authenticating value of these markers.

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource at the University of Colorado School of Medicine, we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies, including HO-1 (Abcam, ab189491; 1:1,000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam, ab46545; 1:100; pH6), 8-OHdG (BioSS, bs-1278R; 1:300; pH9), Ki67 (SP6, 1:400; pH6), and EPCAM (1:200; pH6), followed by horseradish peroxidase–conjugated secondary antibody polymer and HRP-reactive OPAL fluorescent reagents. ..

    Polymer:

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner.
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource (HIMSR) at the University of Colorado School of Medicine we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies HO-1 (Abcam [ab189491]; 1:1000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam [ab46545]; 1:100; pH6), 8-OHdG (BioSS [bs-1278R]; 1:300; pH9), Ki67 ([SP6] 1:400; pH6) and EPCAM (1:200; pH6), followed by horseradish peroxidase-conjugated secondary antibody polymer, and HRP-reactive OPAL fluorescent reagents. ..

    Article Title: Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner
    Article Snippet: Through our collaboration with the Human Immune Monitoring Shared Resource at the University of Colorado School of Medicine, we performed multispectral imaging using the PhenoImager HT instrument (formerly Vectra Polaris, Akoya Biosciences). .. Deparaffinized FFPE tissue sections were heat treated in antigen retrieval buffer, blocked, and incubated with various primary antibodies, including HO-1 (Abcam, ab189491; 1:1,000; pH9), PTGS2 (Cell Marque; 1:50; pH6), 4-HNE (Abcam, ab46545; 1:100; pH6), 8-OHdG (BioSS, bs-1278R; 1:300; pH9), Ki67 (SP6, 1:400; pH6), and EPCAM (1:200; pH6), followed by horseradish peroxidase–conjugated secondary antibody polymer and HRP-reactive OPAL fluorescent reagents. ..

    Labeling:

    Article Title: PKM2 preconditioning protects endothelial cells from pyroptosis and BBB disruption via NRF2/TRX/TXNIP signaling in neonatal hypoxic-ischemic brain injury.
    Article Snippet: .. For double-immunofluorescence labeling, the sections were incubated with antibodies against CD31 (R&D Systems Catalog #AF3628, 1:100), caspase-1 (Abcam; Catalog #ab179515, 1:200), ZO-1 (Abcam; Catalog #ab221547, 1:100), MMP2 (Affinity; Catalog #AF5228, 1:200), NRF2 (Proteintech; Catalog #16396-1- AP, 1:200), 8-OHdG (Bioss; Catalog #bs-1278R, 1:200) or PKM2 (ZENBIO; Catalog #381318, 1:100) overnight at 4 °C. .. Then, the sections were washed, treated with Alexa Fluor 488 or Alexa Fluor 594 secondary antibodies for 1 h, and incubated with 4,6-diamidino-2-phenylindole (DAPI) for 10 min. A Nikon fluorescence microscope was used to capture images.

    Article Title: PKM2 preconditioning protects endothelial cells from pyroptosis and BBB disruption via NRF2/TRX/TXNIP signaling in neonatal hypoxic-ischemic brain injury
    Article Snippet: .. For double-immunofluorescence labeling, the sections were incubated with antibodies against CD31 (R&D Systems Catalog #AF3628, 1:100), caspase-1 (Abcam; Catalog #ab179515, 1:200), ZO-1 (Abcam; Catalog #ab221547, 1:100), MMP2 (Affinity; Catalog #AF5228, 1:200), NRF2 (Proteintech; Catalog #16396-1-AP, 1:200), 8-OHdG (Bioss; Catalog #bs-1278R, 1:200) or PKM2 (ZENBIO; Catalog #381318, 1:100) overnight at 4 °C. .. Then, the sections were washed, treated with Alexa Fluor 488 or Alexa Fluor 594 secondary antibodies for 1 h, and incubated with 4,6-diamidino-2-phenylindole (DAPI) for 10 min. A Nikon fluorescence microscope was used to capture images.

    Immunostaining:

    Article Title: Hypoxia-induced LONP1 overexpression and mtDNA damage may serve as biomarkers for death from mechanical asphyxia.
    Article Snippet: Due to the absence of reliable signs, the authentication of death from mechanical asphyxia (DMA), commonly seen in forensic pathology, can be rather challenging especially when the criminal case is obscure.. Lon protease-1 (LONP1) is an ATP-dependent serine protease located in mitochondria that participates in maintaining the integrity of the mitochondrial genome and regulating mitochondrial DNA (mtDNA) replication.. In this research, cerebral tissues of corpses with different causes of death were collected to compare the expression of LONP1 and the changes of mtDNA to see the authenticating value of these markers.

    Imaging:

    Article Title: Spatial multi-omics guides ASCT2-targeted delivery of glycolysis inhibitor for cervical cancer suppression and chemoresistance reversal.
    Article Snippet: .. Concurrently, tumor sections were subjected to immunofluorescence analysis of Ki-67 (1:200, Servicebio, GB151499), 4-HNE (1:400, Servicebio, GB150073), 8-OhdG (1:200, Bioss, bs-1278r), and MRP2 (1:200, Immunoway YT2840), with imaging performed on a ZEISS Apotome 3 system. .. Additionally, terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) staining was conducted on tumor sections using a commercial kit (Servicebio, G1501) to detect apoptotic cells.

    Blocking Assay:

    Article Title: Oxidative stress-induced astrocytic collagen biosynthesis drives glial barrier formation and neuronal death in ischemic stroke.
    Article Snippet: Both mouse and NHP brain sections were blocked in 0.1 M PBS containing 0.3% Triton X-100 (Sigma) and 5% normal donkey serum (GeneTex) for 1 hour at room temperature. .. The primary antibodies, including GFAP (Sigma-Aldrich, AB5541, 1:500), NeuN (Millipore, ABN90, 1:500), Collagen I (Abcam, ab21286, 1:200), SPARC (Invitrogen, 33–5500, 1:200), Iba1 (Wako, 019-19741, 1:500), 8-OHdG (Bioss, bs-1278R, 1:500), Cleaved Caspase-3 (Cell Signaling, 9661, 1:500), Nitrotyrosine (Invitrogen, A21285, 1:200) and iNOS (Abcam, ab15323, 1:500) in blocking solution were immunostained. ..



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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    8 Ohdg Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Rabbit Anti 8 Ohdg, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    MedChemExpress hy p81140
    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. <t>(H)</t> <t>8-OHdG</t> levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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    Image Search Results


    Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. (H) 8-OHdG levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Aluminum exposure impairs nuclear envelope breakdown for mouse zygote formation

    doi: 10.1016/j.isci.2026.115807

    Figure Lengend Snippet: Aluminum exposure triggers mitochondrial dysfunction, oxidative damage accumulation and apoptosis in mouse zygotes (A) Representative images of mitochondrial distribution in zygotes of the control and treatment groups by immunofluorescence staining. Red, mitochondria; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity and abnormal rate of mitochondria. The relative fluorescence intensity: control, n = 47; AlCl 3 , n = 50; ∗∗ p < 0.005; abnormal rate: control, n = 47; AlCl 3 , n = 50; ∗∗∗ p < 0.0005. (C) TMRE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, TMRE; blue, DNA. Scale bars, 20 μm. (D) Relative fluorescence intensity of TMRE. Control, n = 45; AlCl 3 , n = 46; ∗ p < 0.05. (E) ROS-positive rate in zygotes of the control and treatment groups at 5.5 h, 6 h, 9 h, and 12 h post-fertilization by immunofluorescence staining. Green, ROS. Scale bars, 80 μm. (5.5 h corresponds to 30 min after AlCl 3 treatment, 6 h to 1 h, 9 h–4 h, and 12 h–7 h after AlCl 3 treatment). (F) ROS-positive rate in zygotes at different time points post-fertilization. 5.5 h: control, n = 39; AlCl 3 , n = 38; ∗∗∗ p < 0.0005; 6 h: control, n = 45; AlCl 3 , n = 47; ∗∗ p < 0.005; 9 h: control, n = 41; AlCl 3 , n = 42; ∗ p < 0.05; 12 h: control, n = 38; AlCl 3 , n = 46; ∗∗∗∗ p < 0.0001. (G) The mRNA expression level of Cox1 . ∗∗∗ p < 0.0005. (H) 8-OHdG levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 8-OHdG; blue, DNA. Scale bars, 20 μm. (I) Relative fluorescence intensity of 8-OHdG. Control, n = 41; AlCl 3 , n = 39; ∗∗ p < 0.005. (J) The 4-HNE levels in zygotes of the control and treatment groups by immunofluorescence staining. Red, 4-HNE; blue, DNA. Scale bars, 20 μm. (K) Relative fluorescence intensity of 4-HNE. Control, n = 39; AlCl 3 , n = 41; ∗∗∗∗ p < 0.0001. (L) The mRNA expression levels of oxidative stress-related genes. ∗∗ p < 0.005, ∗∗ p < 0.01, ∗ p < 0.05, ∗∗∗ p < 0.0005. (M) Representative images of early apoptosis in control and AlCl 3 -exposed groups by immunofluorescence staining. Green, annexin-V. Scale bars, 20 μm. (N) Apoptosis rate of zygotes. Control, n = 56; AlCl 3 , n = 50; ∗∗ p < 0.005. (O) The mRNA expression levels of apoptosis-related genes. ∗∗ p < 0.01, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: 8-OHdG antibody , MedChemExpress , Cat# HY- P81140 ; RRID: AB_3103069.

    Techniques: Control, Immunofluorescence, Staining, Fluorescence, Expressing