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fetal calf serum fcs  (ATCC)


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    ATCC fetal calf serum fcs
    Fetal Calf Serum Fcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5a+medium/McCoy's+5A+Medium/pm42881257-288-15-4
    Average 99 stars, based on 2472 article reviews
    fetal calf serum fcs - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Generalizable, high-throughput image analysis of subcellular structures using dispersion indices
    Article Snippet: U-2 OS (Cat# HTB-96, RRID:CVCL_0042) cells were sourced from ATCC. .. U-2 OS cells were cultured with McCoy’s 5A Medium (ATCC) supplemented with 1% penicillin-streptomycin and 10% Fetal Bovine Serum. ..

    Article Title: Dependency Map correlation analysis reveals WDR89 as a genome maintenance factor
    Article Snippet: .. Cell culture and siRNA knockdown U2OS (ATCC) and HCT116 cells were grown in McCoy’s 5A medium, HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM), with all media supplemented with 10% fetal bovine serum. .. siRNA treatments were performed using Qiagen Flexitube siRNAs at 50 nM concentration with RNAiMax transfection reagent for 72 hours before fixation and/or protein harvest.

    Article Title: Rapid Optimization Enabled by Single-Molecule Tracking: Discovery of a Potent RUVBL1/2 Inhibitor to Evaluate the Targeting of MYC-Driven Cancers.
    Article Snippet: .. HCT116 and Ramos cell lines (ATCC) were cultured in McCoy’s 5A Medium and RPMI-1640 Medium, respectively, each supplemented with 10% FBS. .. HCT116 tumors were established in 4−5 week-old female Crl/NU(NCr)-Foxn1nu athymic nude mice, and Ramos tumors in 4−5 week-old female NOD.CB17-Prkdcscid/ NCrCrl NOD-SCID mice (Charles River Laboratories).

    Knockdown:

    Article Title: Dependency Map correlation analysis reveals WDR89 as a genome maintenance factor
    Article Snippet: .. Cell culture and siRNA knockdown U2OS (ATCC) and HCT116 cells were grown in McCoy’s 5A medium, HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM), with all media supplemented with 10% fetal bovine serum. .. siRNA treatments were performed using Qiagen Flexitube siRNAs at 50 nM concentration with RNAiMax transfection reagent for 72 hours before fixation and/or protein harvest.

    Modification:

    Article Title: Dependency Map correlation analysis reveals WDR89 as a genome maintenance factor
    Article Snippet: .. Cell culture and siRNA knockdown U2OS (ATCC) and HCT116 cells were grown in McCoy’s 5A medium, HEK293T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM), with all media supplemented with 10% fetal bovine serum. .. siRNA treatments were performed using Qiagen Flexitube siRNAs at 50 nM concentration with RNAiMax transfection reagent for 72 hours before fixation and/or protein harvest.

    Article Title: Dual Pathways of Extracellular ATP Action in Cancer Cells: Purinergic Signaling–Driven Senescence and Macropinocytic ATP Internalization
    Article Snippet: .. Dulbecco’s Modified eagle Medium (DMEM) high glucose (ATCC-30-2002), Eagle’s Minimum Essential Medium (EMEM-ATCC 30-2003), RPMI (ATCC-30-2001), F12 (ATCC-30-2007), Ham’s F12 modified (ATCC-30-2004), McCoy’s 5A medium (ATCC-30-2007), 0.25% Trypsin-EDTA Solution, 1X (ATCC-30-2101), Fetal bovine serum (ATCC-30-2020), and Penicillin-Streptomycin Solution (ATCC-30-2300) were purchased from ATCC. .. Adenosine 5-triphosphate disodium salt hydrate (A2383), Resazurin sodium salt (R7017), 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) (A3085), IPA3 (I2285), Navitoclax (AMBH2D6F59B5) and 200 mM glutamine (59202C) were purchased from Sigma Aldrich.

    Incubation:

    Article Title: Met32 governs transcriptional control of sulfur metabolic flexibility and resistance to reactive sulfur species in the human fungal pathogen Candida albicans
    Article Snippet: .. Human colon epithelial cell line HT-29 (ATCC; HTB-38) was grown in 96-well plates as monolayers in McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS) at 2 × 10 4 cells per well and incubated at 37°C with 5% CO 2 for 24 h. C. albicans cells grown overnight in MDM were pre-cultured in MDM or MDM supplemented with methionine for 6 h prior to infection. ..

    Infection:

    Article Title: Met32 governs transcriptional control of sulfur metabolic flexibility and resistance to reactive sulfur species in the human fungal pathogen Candida albicans
    Article Snippet: .. Human colon epithelial cell line HT-29 (ATCC; HTB-38) was grown in 96-well plates as monolayers in McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS) at 2 × 10 4 cells per well and incubated at 37°C with 5% CO 2 for 24 h. C. albicans cells grown overnight in MDM were pre-cultured in MDM or MDM supplemented with methionine for 6 h prior to infection. ..



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    Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon <t>(HT29)</t> and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.
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    Image Search Results


    Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Journal: Advances in Radiation Oncology

    Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

    doi: 10.1016/j.adro.2026.102051

    Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

    Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated