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anti nedd4l  (Bethyl)


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    Structured Review

    Bethyl anti nedd4l
    Anti Nedd4l, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/513a+t/NEDD4L+Antibody/10__1161_slash_jaha__124__039855-109-16-22
    Average 93 stars, based on 9 article reviews
    anti nedd4l - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Characterization of PGua4, a guanidinium-rich peptoid that delivers IgGs to the cytosol via macropinocytosis
    Article Snippet: The protein samples were separated on 10% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to 0.45-μm-diameter pore-size nitrocellulose membranes (PerkinElmer Life Sciences). .. The membranes were blocked in Tris-buffered saline (20 mM Tris-HCl (pH 7.4) and 150 Mm NaCl) containing 0.1% Tween 20 and 5% nonfat dry milk and incubated at room temperature for 1h with primary polyclonal antibodies (anti-clathrin antibody 1:1000 (#2410, Cell Signaling Technology, Danvers, Massachusetts, USA), anti-caveolin-1 antibody 1:1000 (#3267, Cell Signaling Technology, Danvers, Massachusetts, USA,) or anti-dynamin-2 antibody 1:1000 (#A303-513A-T, Bethyl laboratories inc., Montgomery, TX, USA,). .. The membranes were next incubated with horseradish peroxidase-conjugated (HRP) goat anti-rabbit IgG 1:7000 (Bio-Rad, Hercules, CA, USA) for 45 min at room temperature and enhanced chemiluminescence detection reagent (Pierce).

    Incubation:

    Article Title: Characterization of PGua4, a guanidinium-rich peptoid that delivers IgGs to the cytosol via macropinocytosis
    Article Snippet: The protein samples were separated on 10% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to 0.45-μm-diameter pore-size nitrocellulose membranes (PerkinElmer Life Sciences). .. The membranes were blocked in Tris-buffered saline (20 mM Tris-HCl (pH 7.4) and 150 Mm NaCl) containing 0.1% Tween 20 and 5% nonfat dry milk and incubated at room temperature for 1h with primary polyclonal antibodies (anti-clathrin antibody 1:1000 (#2410, Cell Signaling Technology, Danvers, Massachusetts, USA), anti-caveolin-1 antibody 1:1000 (#3267, Cell Signaling Technology, Danvers, Massachusetts, USA,) or anti-dynamin-2 antibody 1:1000 (#A303-513A-T, Bethyl laboratories inc., Montgomery, TX, USA,). .. The membranes were next incubated with horseradish peroxidase-conjugated (HRP) goat anti-rabbit IgG 1:7000 (Bio-Rad, Hercules, CA, USA) for 45 min at room temperature and enhanced chemiluminescence detection reagent (Pierce).



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