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48-well mea plates  (Axion BioSystems)

 
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    Axion BioSystems 48-well mea plates
    48 Well Mea Plates, supplied by Axion BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/48-well+meas/48+well+mea+plates/pmc09481914__mmc5-39-33-35
    Average 90 stars, based on 1 article reviews
    48-well mea plates - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: A prefrontal cortex alpha/delta switch controls the transition from positive to negative affective states
    Article Snippet: To determine the dose of JB2 (IGF2R positive modulator, 20), 21 days- in-vitro (DIV) primary cortical neurons were analyzed 1 h after JB2 administration for field potential recordings by 48-well MEAs (Maestro, Axion biosystems).

    Article Title: Phenotypic Screening of Prospective Analgesics Among FDA‐Approved Compounds using an iPSC‐Based Model of Acute and Chronic Inflammatory Nociception
    Article Snippet: 48‐well MEAs from Axion Biosystems (Atalanta, GA) were first pre‐treated overnight with 3 to 5 μl of 0.1% polyethyleneimine (PEI, Sigma‐Aldrich, St. Louis, MO), washed twice using DI water, allowed to dry, and then coated with 3 to 5 μl of 20 μg ml −1 laminin (Sigma‐Aldrich, St. Louis, MO) for at least 1 h. Laminin was aspirated immediately prior to seeding and substrates were not allowed to dry completely. hiPSC glia (BX‐0600, BrainXell, Madison, WI) and hiPSC SN (Anatomic, Minneapolis, MN) were thawed rapidly, separately diluted in 37 °C DMEM (ThermoFisher Scientific, Waltham, MA), centrifuged at 300× g for 5 min, and reconstituted in supplemented SN medium (Anatomic's Senso‐MM without Inhibitors plus 1:1000 BrainXell astrocyte supplement).

    Article Title: Functional Characterization of Human Pluripotent Stem Cell-Derived Models of the Brain with Microelectrode Arrays
    Article Snippet: The approach has even been expanded to the high-throughput screening of organoids by plating them on 48-well MEAs (Axion Biosystems) [ ].

    Article Title: Phenotypic Screening of Prospective Analgesics Among FDA-Approved Compounds using an iPSC-Based Model of Acute and Chronic Inflammatory Nociception.
    Article Snippet: 48-well MEAs from Axion Biosystems (Atalanta, GA) were first pre-treated overnight with 3 to 5 μl of 0.1% polyethyleneimine (PEI, Sigma-Aldrich, St. Louis, MO), washed twice using DI water, allowed to dry, and then coated with 3 to 5 μl of 20 μg ml−1 laminin (Sigma-Aldrich, St. Louis, MO) for at least 1 h. Laminin was aspirated immediately prior to seeding and substrates were not allowed to dry completely. hiPSC glia (BX-0600, BrainXell, Madison, WI) and hiPSC SN (Anatomic, Minneapolis, MN) were thawed rapidly, separately diluted in 37 °C DMEM (ThermoFisher Scientific, Waltham, MA), centrifuged at 300× g for 5 min, and reconstituted in supplemented SN medium (Anatomic’s Senso-MM without Inhibitors plus 1:1000 BrainXell astrocyte supplement).

    Article Title: A prefrontal cortex alpha/delta switch controls the transition from positive to negative affective states.
    Article Snippet: To determine the dose of JB2 (IGF2R positive modulator, 20), 21 days-in-vitro (DIV) primary cortical neurons were analyzed 1 h after JB2 administration for field potential recordings by 48-well MEAs (Maestro, Axion biosystems).

    Article Title: An IGFBP2-derived peptide promotes neuroplasticity and rescues deficits in a mouse model of Phelan-McDermid syndrome.
    Article Snippet: We developed an IGFBP2-mimetic peptide fragment, JB2, and showed that it promotes basal synaptic structural and functional plasticity in cultured neurons and mice.. We demonstrate that JB2 directly binds to dendrites and synapses, and its biological activity involves NMDA receptor activation, gene transcription and translation, and IGF2 receptors.. It is not IGF1 receptor-dependent.



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    How MEA was used to study NDD with hiPSC model.

    Journal: Frontiers in Neuroscience

    Article Title: Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders

    doi: 10.3389/fnins.2024.1524577

    Figure Lengend Snippet: How MEA was used to study NDD with hiPSC model.

    Article Snippet: MEF2C deficiency , , 2d cortical excitatory neurons (NGN2 transduction) , 48-well CytoView LD-MEA (Axion Biosystem) , Every 3 days from day 27 to day 60 , BrainPhys , N/A , Weighted MFR, spike count, synchrony, oscillation , Neurons with loss of MEF2C displayed decreased network activity and synchrony, suggesting a disruption in the synapse formation..

    Techniques: Control, Transduction, Activity Assay, Disruption, Mutagenesis, Imaging, Inhibition, In Vitro