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traf1 45d3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc traf1 45d3
    Traf1 45d3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/45d3/TRAF1+Rabbit+mAb/pmc12618069__jci-135-186258-s037-128-30-32
    Average 94 stars, based on 39 article reviews
    traf1 45d3 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Protein Binding:

    Article Title: Anti-Fn14 Antibody-Conjugated Nanoparticles Display Membrane TWEAK-Like Agonism †
    Article Snippet: .. In brief, free protein binding sites on the nitrocellulose were blocked for 1 h in Tris-buffered saline containing 0.1% Tween 20 and 5% dry milk and proteins of interest were detected via sequential incubation with primary antibodies: (anti-TRAF1 mAb, (45D3) #4715, Source: Rabbit; anti-TRAF2 mAb, Source: Rabbit, (C192), #4724) from Cell Signaling, MA, USA; anti-NFκB p100/p52 mAb, Source: Mouse, #05-361 from Millipore, Darmstadt, Germany; anti-ß-actin mAb, Source: Mouse, clone AC-15, #A1978 from Sigma-Aldrich, Schnelldorf, Germany, horseradish peroxidase(HRP)-conjugated secondary antibodies: (Anti-Mouse Immunoglobulins/HRP, Source: Rabbit, #P0260 from Dako, Hamburg, Germany; Anti-rabbit IgG, HRP-linked Antibody, Source: Goat, #7074, Cell Signaling, MA, USA), and the commercially available ECL western blotting detection reagents and analysis system (Amersham Biosciences, Muenchen, Germany). ..

    Saline:

    Article Title: Anti-Fn14 Antibody-Conjugated Nanoparticles Display Membrane TWEAK-Like Agonism †
    Article Snippet: .. In brief, free protein binding sites on the nitrocellulose were blocked for 1 h in Tris-buffered saline containing 0.1% Tween 20 and 5% dry milk and proteins of interest were detected via sequential incubation with primary antibodies: (anti-TRAF1 mAb, (45D3) #4715, Source: Rabbit; anti-TRAF2 mAb, Source: Rabbit, (C192), #4724) from Cell Signaling, MA, USA; anti-NFκB p100/p52 mAb, Source: Mouse, #05-361 from Millipore, Darmstadt, Germany; anti-ß-actin mAb, Source: Mouse, clone AC-15, #A1978 from Sigma-Aldrich, Schnelldorf, Germany, horseradish peroxidase(HRP)-conjugated secondary antibodies: (Anti-Mouse Immunoglobulins/HRP, Source: Rabbit, #P0260 from Dako, Hamburg, Germany; Anti-rabbit IgG, HRP-linked Antibody, Source: Goat, #7074, Cell Signaling, MA, USA), and the commercially available ECL western blotting detection reagents and analysis system (Amersham Biosciences, Muenchen, Germany). ..

    Incubation:

    Article Title: Anti-Fn14 Antibody-Conjugated Nanoparticles Display Membrane TWEAK-Like Agonism †
    Article Snippet: .. In brief, free protein binding sites on the nitrocellulose were blocked for 1 h in Tris-buffered saline containing 0.1% Tween 20 and 5% dry milk and proteins of interest were detected via sequential incubation with primary antibodies: (anti-TRAF1 mAb, (45D3) #4715, Source: Rabbit; anti-TRAF2 mAb, Source: Rabbit, (C192), #4724) from Cell Signaling, MA, USA; anti-NFκB p100/p52 mAb, Source: Mouse, #05-361 from Millipore, Darmstadt, Germany; anti-ß-actin mAb, Source: Mouse, clone AC-15, #A1978 from Sigma-Aldrich, Schnelldorf, Germany, horseradish peroxidase(HRP)-conjugated secondary antibodies: (Anti-Mouse Immunoglobulins/HRP, Source: Rabbit, #P0260 from Dako, Hamburg, Germany; Anti-rabbit IgG, HRP-linked Antibody, Source: Goat, #7074, Cell Signaling, MA, USA), and the commercially available ECL western blotting detection reagents and analysis system (Amersham Biosciences, Muenchen, Germany). ..

    Western Blot:

    Article Title: Anti-Fn14 Antibody-Conjugated Nanoparticles Display Membrane TWEAK-Like Agonism †
    Article Snippet: .. In brief, free protein binding sites on the nitrocellulose were blocked for 1 h in Tris-buffered saline containing 0.1% Tween 20 and 5% dry milk and proteins of interest were detected via sequential incubation with primary antibodies: (anti-TRAF1 mAb, (45D3) #4715, Source: Rabbit; anti-TRAF2 mAb, Source: Rabbit, (C192), #4724) from Cell Signaling, MA, USA; anti-NFκB p100/p52 mAb, Source: Mouse, #05-361 from Millipore, Darmstadt, Germany; anti-ß-actin mAb, Source: Mouse, clone AC-15, #A1978 from Sigma-Aldrich, Schnelldorf, Germany, horseradish peroxidase(HRP)-conjugated secondary antibodies: (Anti-Mouse Immunoglobulins/HRP, Source: Rabbit, #P0260 from Dako, Hamburg, Germany; Anti-rabbit IgG, HRP-linked Antibody, Source: Goat, #7074, Cell Signaling, MA, USA), and the commercially available ECL western blotting detection reagents and analysis system (Amersham Biosciences, Muenchen, Germany). ..



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    Cell Signaling Technology Inc rabbit anti traf1 45d3 monoclonal antibody
    Ectopic expression of CagA increases the expression of <t>TRAF1</t> and 4-1BB in GES-1 cells. (A) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of TRAF1 was determined using RT-qPCR analysis with GAPDH as the internal control. (B) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of 4-1BB was determined using RT-qPCR analysis with GAPDH as the internal control. (C) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 and 72 h. TRAF1 and 4-1BB protein levels were determined using western blot analysis with GAPDH as the loading control. (D) Relative expression of TRAF1 compared with GAPDH. (E) Relative expression of 4-1BB compared with GAPDH. *P<0.05, **P<0.01, ***P<0.001. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; EGFP, enhanced green fluorescent protein; CagA, cytotoxin-associated gene A; TRAF1, tumor necrosis factor receptor-associated factor 1; 4-1BB, tumor necrosis factor receptor superfamily member 9.
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    Ectopic expression of CagA increases the expression of TRAF1 and 4-1BB in GES-1 cells. (A) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of TRAF1 was determined using RT-qPCR analysis with GAPDH as the internal control. (B) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of 4-1BB was determined using RT-qPCR analysis with GAPDH as the internal control. (C) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 and 72 h. TRAF1 and 4-1BB protein levels were determined using western blot analysis with GAPDH as the loading control. (D) Relative expression of TRAF1 compared with GAPDH. (E) Relative expression of 4-1BB compared with GAPDH. *P<0.05, **P<0.01, ***P<0.001. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; EGFP, enhanced green fluorescent protein; CagA, cytotoxin-associated gene A; TRAF1, tumor necrosis factor receptor-associated factor 1; 4-1BB, tumor necrosis factor receptor superfamily member 9.

    Journal: Molecular Medicine Reports

    Article Title: CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB

    doi: 10.3892/mmr.2017.6757

    Figure Lengend Snippet: Ectopic expression of CagA increases the expression of TRAF1 and 4-1BB in GES-1 cells. (A) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of TRAF1 was determined using RT-qPCR analysis with GAPDH as the internal control. (B) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 h. The mRNA level of 4-1BB was determined using RT-qPCR analysis with GAPDH as the internal control. (C) GES-1 cells were transfected with plasmid pEGFP-C1/CagA or control vector pEGFP-C1 for 48 and 72 h. TRAF1 and 4-1BB protein levels were determined using western blot analysis with GAPDH as the loading control. (D) Relative expression of TRAF1 compared with GAPDH. (E) Relative expression of 4-1BB compared with GAPDH. *P<0.05, **P<0.01, ***P<0.001. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; EGFP, enhanced green fluorescent protein; CagA, cytotoxin-associated gene A; TRAF1, tumor necrosis factor receptor-associated factor 1; 4-1BB, tumor necrosis factor receptor superfamily member 9.

    Article Snippet: Rabbit anti-CD137 polyclonal antibody was purchased from Abcam (Cambridge, UK; cat. no. ab203391); rabbit anti-TRAF1 (45D3) monoclonal antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. no. 4715); rabbit anti-CagA (cat. no. sc-25766), goat anti-rabbit immunoglobulin (Ig)G-horseradish peroxidase (HRP; cat. no. sc-2030) and goat anti-mouse IgG-HRP (cat. no. sc-2302) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); and mouse anti-GAPDH monoclonal antibody (cat. no. MAB374) was purchased from Merck KGaA (Darmstadt, Germany).

    Techniques: Expressing, Transfection, Plasmid Preparation, Control, Quantitative RT-PCR, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction