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Pyrosequencing Inc 454 gs flx pyrosequencing technology
454 Gs Flx Pyrosequencing Technology, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/454-flx+pyrosequencing/454+picotiter+pyrosequencing+technology/pm41894116-30-28-31
Average 86 stars, based on 1 article reviews
454 gs flx pyrosequencing technology - by Bioz Stars, 2026-10
86/100 stars

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cDNA Library Assay:

Article Title: Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection.
Article Snippet: .. The resulting cDNA library, with an average sequence length of 949 nt, was subjected to 454-FLX pyrosequencing. ..

Sequencing:

Article Title: Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection.
Article Snippet: .. The resulting cDNA library, with an average sequence length of 949 nt, was subjected to 454-FLX pyrosequencing. ..

other:

Article Title: Seasonal characteristics of odor and methane mitigation and the bacterial community dynamics in an on-site biocover at a sanitary landfill.
Article Snippet: Landfills are key anthropogenic emission sources for odors and methane.. For simultaneous mitigation of odors and methane emitted from landfills, a pilot-scale biocover (soil:perlite:earthworm cast:compost, 6:2:1:1, v/v) was constructed at a sanitary landfill in South Korea, and the biocover performance and its bacterial community dynamics were monitored for 240 days.. The removal efficiencies of odor and methane were evaluated to compare the odor dilution ratios or methane concentrations at the biocover surface and landfill soil cover surface where the biocover was not installed.

Article Title: Comparison of root transcriptomes and expressions of genes involved in main medicinal secondary metabolites from Bupleurum chinense and Bupleurum scorzonerifolium, the two Chinese official Radix bupleuri source species.
Article Snippet: Radix bupleuri, roots of Bupleurum species, is a widely used traditional Chinese medicine.. Here, we compared the root transcriptomes of both Bupleurum chinense DC. and B. scorzonerifolium Willd.. A total of 313 483 and 342 263 high quality expressed sequence tags were obtained respectively.

Article Title: Fecal microbiome of growing pigs fed a cereal based diet including chicory ( Cichorium intybus L.) or ribwort ( Plantago lanceolata L.) forage
Article Snippet: The reverse primer was tagged with 1 of 4 labels (CGAT, CATG, CTGA and CGTA) at the 5′ end along with the adaptor sequence (5′- GCCTCCCTCGCGCCATCAG 3′) to allow 4 samples to be included in a single 454-FLX pyrosequencing lane as previously described [ ].

Article Title: Fecal microbiome of growing pigs fed a cereal based diet including chicory ( Cichorium intybus L.) or ribwort ( Plantago lanceolata L.) forage
Article Snippet: 454-FLX pyrosequencing of 16S rRNA gene amplicons was used to analyze the microbial composition in a subset of the samples already analyzed with T-RFLP.

Article Title: De novo assembly and comparative analysis of the Ceratodon purpureus transcriptome.
Article Snippet: The bryophytes are a morphologically and ecologically diverse group of plants that have recently emerged as major model systems for a variety of biological processes.. In particular, the genome sequence of the moss, Physcomitrella patens, has significantly enhanced our understanding of the evolution of developmental processes in land plants.. However, to fully explore the diversity within bryophytes, we need additional genomic resources.

Article Title: Effects of nitrate and sulfate on the performance and bacterial community structure of membrane-less single-chamber air-cathode microbial fuel cells.
Article Snippet: To cite this article: Yoonjoo Seo, Hyemin Kang, Sumin Chang, Yun-Yeong Lee & Kyung-Suk Cho (2017): Effects of nitrate and sulfate on the performance and bacterial community structure of membrane-less single-chamber air-cathode microbial fuel cells, Journal of Environmental Science and Health, Part A, DOI: 10.1080/10934529.2017.1366242 To link to this article: http://dx.doi.org/10.1080/10934529.2017.1366242

Article Title: Mendelian randomization analysis reveals causal relationship between tonsillectomy and irritable bowel syndrome
Article Snippet: Through high throughput bar-coded 454-FLX pyrosequencing, the composition and structure of the tonsillar microbiota in healthy pigs were comprehensively analyzed, revealing the predominant role of the Pasteurellaceae family in the tonsillar microbial community ( ).



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Overview of sequencing technologies 2004–2022. Post-Sanger sequencing technologies, beginning with <t>Roche</t> <t>454</t> Pyrosequencing in 2004, Illumina HiSeq and MiSeq (2007), SOLiD (2008), PacBioSMRT (2009), DNBS: Helicos (2010), Ion Torrent (2011), in situ RNA sequencing (2013), ONT nanopore (2015), spatial transcriptomics (2016), GeoSeq (2017), Slide-Seq (2019), and Revio (2022), illustrate the continuous improvements contributing to the genomics. It explores the various NGS sequencing techniques that have expanded upon chain termination and polymerase chain reaction.
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Overview of sequencing technologies 2004–2022. Post-Sanger sequencing technologies, beginning with Roche 454 Pyrosequencing in 2004, Illumina HiSeq and MiSeq (2007), SOLiD (2008), PacBioSMRT (2009), DNBS: Helicos (2010), Ion Torrent (2011), in situ RNA sequencing (2013), ONT nanopore (2015), spatial transcriptomics (2016), GeoSeq (2017), Slide-Seq (2019), and Revio (2022), illustrate the continuous improvements contributing to the genomics. It explores the various NGS sequencing techniques that have expanded upon chain termination and polymerase chain reaction.

Journal: Genes

Article Title: A Comprehensive Review of Bioinformatics Tools for Genomic Biomarker Discovery Driving Precision Oncology

doi: 10.3390/genes15081036

Figure Lengend Snippet: Overview of sequencing technologies 2004–2022. Post-Sanger sequencing technologies, beginning with Roche 454 Pyrosequencing in 2004, Illumina HiSeq and MiSeq (2007), SOLiD (2008), PacBioSMRT (2009), DNBS: Helicos (2010), Ion Torrent (2011), in situ RNA sequencing (2013), ONT nanopore (2015), spatial transcriptomics (2016), GeoSeq (2017), Slide-Seq (2019), and Revio (2022), illustrate the continuous improvements contributing to the genomics. It explores the various NGS sequencing techniques that have expanded upon chain termination and polymerase chain reaction.

Article Snippet: For example, Roche/454 FLX Pyrosequencer was the first high-throughput screening platform used in 2004 [ , ].

Techniques: Sequencing, In Situ, RNA Sequencing Assay, Polymerase Chain Reaction

Methodology of included studies

Journal: BMC Oral Health

Article Title: The role of oral microbiota in the development of oral mucositis in pediatric oncology patients treated with antineoplastic drugs: a systematic review

doi: 10.1186/s12903-024-03938-y

Figure Lengend Snippet: Methodology of included studies

Article Snippet: Ye 2013 [ ] , Bacterial samples analyzed using 454 FLX pyrosequencing with minor modifications. The PCR products were sequenced using a two-lane PicoTiterPlate on a Genome Sequencer FLX system. Denoised sequences were aligned and sorted into operational taxonomic units (OTUs). , Qualitative analysis Prevalence Quantitative analysis (Unifrac).

Techniques: Bacteria, Control, Chocolate, Modification, Staining, Biomarker Discovery