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454 sequencing data  (DNASTAR)


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    Structured Review

    DNASTAR 454 sequencing data
    454 Sequencing Data, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/454+sequencing+data/Lasergene/us11326178-998-27-35
    Average 99 stars, based on 10685 article reviews
    454 sequencing data - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title:
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. ..

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/mlKanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CCACTCGGTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Hibiscus cannabinus (Kenaf) stem sections were collected and stored in RNAlater (Qiagen) until processing.

    Article Title:
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title:
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO: 14) and 5′-TTCACAGCITTTCGAGAGCGGTC-3′ (SEQ ID NO: 15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Total RNA was extracted by adding 100 mg of powdered Hibiscus cannabinus stem sections to 1 ml Trizol buffer (Invitrogen) and incubating for 15 minutes while vortexing at room temperature.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. After cooling on ice, 2.5 μL of the reaction was used to transform One Shot Top 10 Chemically Competent E. coli Cells (Invitrogen) according to manufacturer's guidelines.

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Software:

    Article Title:
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. ..

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/mlKanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CCACTCGGTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Hibiscus cannabinus (Kenaf) stem sections were collected and stored in RNAlater (Qiagen) until processing.

    Article Title:
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title:
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO: 14) and 5′-TTCACAGCITTTCGAGAGCGGTC-3′ (SEQ ID NO: 15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Total RNA was extracted by adding 100 mg of powdered Hibiscus cannabinus stem sections to 1 ml Trizol buffer (Invitrogen) and incubating for 15 minutes while vortexing at room temperature.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen), along with 5′-CGCACTCGGTTTGTGATGGC-3′ (SEQ ID NO:14) and 5′-TTCACAGCTTTCGAGAGCGGTC-3′ (SEQ ID NO:15) as two gene specific primers, at the Michigan State University Genomics Core. .. This sequence data was compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 Sequence Manager software. .. Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. After cooling on ice, 2.5 μL of the reaction was used to transform One Shot Top 10 Chemically Competent E. coli Cells (Invitrogen) according to manufacturer's guidelines.

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Next-Generation Sequencing:

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/mlKanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title:
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.

    Article Title: P-coumaroyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. After cooling on ice, 2.5 μL of the reaction was used to transform One Shot Top 10 Chemically Competent E. coli Cells (Invitrogen) according to manufacturer's guidelines.

    Article Title: Feruloyl-CoA:monolignol transferase
    Article Snippet: Single colonies were picked and grown in LB media containing 50 μg/ml Kanamycin overnight at 37° C. Plasmid DNA was purified from these cultures using the QIAprep Spin Miniprep Kit (Qiagen), according to manufacturer's guidelines. .. Samples were submitted for high throughput sequencing, using the M13 forward and M13 reverse primers (Invitrogen) at the Michigan State University Genomics Core, and compared to the 454 sequencing data to verify coding sequence using DNASTAR Lasergene 8 software. .. Sequences in entry vectors were inserted into pDEST17 vector using 150 ng of plasmid DNA from the entry clone, 150 ng of pDEST17 vector and 1 μL LR Clonase II Enzyme Mix.



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    Emergence percentage of N. <t>triangulifer</t> subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)
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    Image Search Results


    Emergence percentage of N. triangulifer subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)

    Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

    Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

    doi: 10.1098/rstb.2018.0013

    Figure Lengend Snippet: Emergence percentage of N. triangulifer subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)

    Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

    Techniques:

    (a) 35SO4 uptake rates in larvae of the mayfly N. triangulifer as a function of SO4 concentrations (re-drawn from Scheibener et al. [30]). (b) Total body S concentrations in subimagos reared for their entire larval stage at different SO4 concentrations.

    Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

    Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

    doi: 10.1098/rstb.2018.0013

    Figure Lengend Snippet: (a) 35SO4 uptake rates in larvae of the mayfly N. triangulifer as a function of SO4 concentrations (re-drawn from Scheibener et al. [30]). (b) Total body S concentrations in subimagos reared for their entire larval stage at different SO4 concentrations.

    Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

    Techniques:

    Relative mRNA expression of N. triangulifer larvae subjected to 24 h sulfate stress treatment. All data are normalized to control (ASW). Error bars represent standard deviations from the mean (n = 6). A Student's t-test was performed and an asterisk indicates significant difference compared to ASW control. (Online version in colour.)

    Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

    Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

    doi: 10.1098/rstb.2018.0013

    Figure Lengend Snippet: Relative mRNA expression of N. triangulifer larvae subjected to 24 h sulfate stress treatment. All data are normalized to control (ASW). Error bars represent standard deviations from the mean (n = 6). A Student's t-test was performed and an asterisk indicates significant difference compared to ASW control. (Online version in colour.)

    Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

    Techniques: Expressing, Control