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Dendrogram showing the similarity among RAPD ‐ PCR patterns of Lactobacillus plantarum ATCC 14917 T (A), Enterococcus faecium DSMZ 20477T (B), (C) Saccharomyces cerevisiae S441 and Escherichia coli PQ 37 (D) after exposure to toxic compounds tested. Similarities were calculated using UPGMA . Arbitrary threshold 90% was used to identify new adducted biotypes. n.c.: negative control.

Journal: Microbial Biotechnology

Article Title: Food borne bacterial models for detection of benzo[a]pyrene‐ DNA adducts formation using RAPD ‐ PCR

doi: 10.1111/1751-7915.12355

Figure Lengend Snippet: Dendrogram showing the similarity among RAPD ‐ PCR patterns of Lactobacillus plantarum ATCC 14917 T (A), Enterococcus faecium DSMZ 20477T (B), (C) Saccharomyces cerevisiae S441 and Escherichia coli PQ 37 (D) after exposure to toxic compounds tested. Similarities were calculated using UPGMA . Arbitrary threshold 90% was used to identify new adducted biotypes. n.c.: negative control.

Article Snippet: In this study, Lactobacillus plantarum ATCC 14917T, Enterococcus faecium DSMZ 20477T, Escherichia coli PQ 37 and Saccharomyces cerevisiae S441 were screened for DNA genetic alterations by DNA fingerprinting using M13 and LA 1 primers after treatment with three compounds forming covalent adducts with DNA [benzo[a]pyrenediol epoxide ( BPDE ), methyl methanesulfonate and 1,2,3,4‐diepoxybutane ( DEB )].

Techniques: Negative Control