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2i lif medium  (Tocris)


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    Tocris 2i lif medium
    2i Lif Medium, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif+medium/PD+0325901/pm41219433-330-10-19
    Average 96 stars, based on 412 article reviews
    2i lif medium - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Reprogramming of H3K36me2 guides lineage-specific post-implantation de novo DNA methylation.
    Article Snippet: In mammals, DNA methylation is re-established after implantation following post-fertilization global erasure.. Yet, the underlying mechanism remains elusive.. Here we investigate H3K36me2 reprogramming in mouse early development and its role in post-implantation DNA methylation re-establishment.

    Article Title: Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting
    Article Snippet: R1 mESCs were cultured on 0.1% gelatin-coated dishes in Dulbecco’s modified Eagle’s medium (Gibco, catalog no. 11995065) containing 15% FBS (Hyclone, SH30396.03), leukemia inhibitory factor (LIF) (1000 IU/ml; Merck Millipore, ESG1107), penicillin (100 IU/ml) and streptomycin (100 μg/ml) (Merck Millipore, TMS-AB2-C), 2 mM GlutaMAX (Gibco, 35050061), 1% EmbryoMax nucleosides (Merck Millipore, ES-008-D), 0.1 mM nonessential amino acids (Gibco, 25-025-CIR), and 0.1 mM 2-mercaptoethanol (Gibco, 21985023). .. Rat ESCs were cultured on 0.1% gelatin-coated dishes in 2i/LIF medium [N2B27 medium supplemented with 1 μm of PD0325901 (Tocris, 41921), 3 μm of CHIR99021 (Tocris, 4423), and LIF (1000 IU/ml)]. ..



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    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
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    2i lif medium  (Selleck Chemicals)
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    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
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    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
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    Image Search Results


    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: FBS–LIF, 2i-LIF and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .

    Journal: Experimental & Molecular Medicine

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells

    doi: 10.1038/s12276-026-01655-w

    Figure Lengend Snippet: a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: FBS–LIF, 2i-LIF and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .

    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific).

    Techniques: Western Blot, Molecular Weight, Marker, Expressing, DNA Methylation Assay, Control