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2016 graphpad prism  (Proteintech)


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    Proteintech 2016 graphpad prism
    KEY RESOURCES TABLE
    2016 Graphpad Prism, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2016+graphpad+prism/PELI1+Antibody/pmc08722104-1097-227-235
    Average 94 stars, based on 16 article reviews
    2016 graphpad prism - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses"

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses

    Journal: Immunity

    doi: 10.1016/j.immuni.2020.11.003

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR, Plasmid Preparation, Expressing, Software, Single-cell Analysis

    Related Articles

    Recombinant:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Plasmid Preparation:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Expressing:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Software:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Single-cell Analysis:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Derivative Assay:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Enzyme-linked Immunosorbent Assay:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Sequencing:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    CRISPR:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Retroviral:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021

    Pore Size:

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-B*40:01-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) pLZRS-HLA-C*03:03-IRES-DNGFR Dr. M. Griffioen (LUMC, the Netherlands) (Griffioen et al., 2012; Van Bergen et al., 2010) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji – plugin ThunderSTORM Ovesný et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 mm3 2cm, C18 particle size 5 mm, pore size 100Å.

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.
    Article Snippet: Dionex/Thermo Fisher Scientific Cat# 164199 (Continued on next page) e3 Immunity 54, 1–19.e1–e9, October 12, 2021



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    Journal: Immunity

    Article Title: The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses

    doi: 10.1016/j.immuni.2020.11.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: This paper N/A Recombinant DNA pX330 Addgene Plasmid #42230 TIA-2A-blast Dr. T. Brummelkamp (NKI, the Netherlands) Blomen et al., 2015 LentiCRISPR_v2 Addgene Plasmid #52961 pL-CRISPR.EFS.GFP Addgene Plasmid #57818 pMXs-puro retroviral expression vector Cell Biolabs Cat# RTV-012 pMXs-puro-GFP This paper N/A pMXs-puro-GFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-GFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-N This paper N/A pMXs-puro-FLAG-C This paper N/A pMXs-puro-FLAG-B3GNT5 This paper IMAGE:202800754 pMXs-puro-B3GNT5-FLAG This paper IMAGE:202800754 pMXs-puro-RFP This paper N/A pMXs-puro-RFP-SPPL3 This paper Voss et al., 2012 pMXs-puro-RFP-SPPL3DA This paper Voss et al., 2012 pMXs-puro-FLAG-B4GALNT1 This paper IMAGE:202800771 pMXs-puro-FLAG-ST3GAL5 This paper IMAGE:202759803 puc2CL6IN-HLA-C*05:01 (with mutated signal peptide from HLA-A*02:01 [M4V]) Dr. A. Halenius (University Medical Center Freiburg) N/A pLZRS-HLA-A*02:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-B*40:01-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) pLZRS-HLA-C*03:03-IRES-ΔNGFR Dr. M. Griffioen (LUMC, the Netherlands) ( Griffioen et al., 2012 ; Van Bergen et al., 2010 ) Software and Algorithms Phyre2 http://www.sbg.bio.ic.ac.uk/ Kelley et al., 2015 PyMOL v2.0 http://pymol.org Schrödinger, LCC Compass Data Analysis v4.0 and v5.0 software http://www.bruker.com Bruker Daltonics FlowJo Single Cell Analysis v10 software http://www.flowjo.com FlowJo, LLC Fiji - plugin ThunderSTORM Ovesny et al., 2014 Glycoworkbench Ceroni et al., 2008 Glycomod Cooper et al., 2001 TCGA survival and expression data http://oncoLnc.org Anaya, 2016 Graphpad Prism http://www.graphpad.com N/A Other RFP-Trap_A antibody ChromoTek Cat# rta-20, RRID:AB_2631362 Acclaim PepMap100 C18 column, 100 μm × 2cm, C18 particle size 5 μm, pore size 100υ.

    Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Sequencing, CRISPR, Plasmid Preparation, Expressing, Software, Single-cell Analysis