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βcgrp  (GL Biochem)

 
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    GL Biochem βcgrp
    βcgrp, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2cgrp/%CE%B2cgrp/pm40424582-183-60-64
    Average 90 stars, based on 1 article reviews
    βcgrp - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Unique Biased Agonism Profile of βCGRP on CGRP Family Receptors.
    Article Snippet: αand β-calcitonin gene-related peptides (αCGRP and βCGRP, respectively), together with adrenomedullin (AM) and AM2 are endogenous agonists of the CGRP family of receptors; CGRP receptor (CGRPR), AM1 receptor (AM1R), and AM2 receptor (AM2R).. The high sequence homology and similar tissue distribution of αCGRP and βCGRP suggests they have overlapping physiological roles in pain pathways, inflammation, and metabolism, but recent data indicate potential differences in the signaling capabilities of these peptides.. However, a comprehensive pharmacological characterization of βCGRP activity, compared to αCGRP, AM, and AM2 across the three CGRP family receptors, is lacking.



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    A , B The expression of <t>βCGRP,</t> <t>BAX,</t> PPARɤ, and TGFβ was higher in BLM and Calca +/− rats compared to WT group (_400). There was abnormal expression of CD3, CD68, βCGRP, BAX, PPARɤ and TGFβ in the BLM group and Calca +/− group. The TUNEL staining showed there were more positive alveolar epithelial cells in BLM group and Calca +/− group than in WT control group (_200). Predominant CD68+CD206+M2 subtype differentiation was found in both Calca +/− rats and BLM rats by immunofluorescence staining (In C , green represents CD68+ and red represents iNOS. In D , green represents CD68+ and red represents CD206+)(_200).
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    Image Search Results


    A , B The expression of βCGRP, BAX, PPARɤ, and TGFβ was higher in BLM and Calca +/− rats compared to WT group (_400). There was abnormal expression of CD3, CD68, βCGRP, BAX, PPARɤ and TGFβ in the BLM group and Calca +/− group. The TUNEL staining showed there were more positive alveolar epithelial cells in BLM group and Calca +/− group than in WT control group (_200). Predominant CD68+CD206+M2 subtype differentiation was found in both Calca +/− rats and BLM rats by immunofluorescence staining (In C , green represents CD68+ and red represents iNOS. In D , green represents CD68+ and red represents CD206+)(_200).

    Journal: Genes and Immunity

    Article Title: αCGRP deficiency aggravates pulmonary fibrosis by activating the PPARγ signaling pathway

    doi: 10.1038/s41435-023-00206-x

    Figure Lengend Snippet: A , B The expression of βCGRP, BAX, PPARɤ, and TGFβ was higher in BLM and Calca +/− rats compared to WT group (_400). There was abnormal expression of CD3, CD68, βCGRP, BAX, PPARɤ and TGFβ in the BLM group and Calca +/− group. The TUNEL staining showed there were more positive alveolar epithelial cells in BLM group and Calca +/− group than in WT control group (_200). Predominant CD68+CD206+M2 subtype differentiation was found in both Calca +/− rats and BLM rats by immunofluorescence staining (In C , green represents CD68+ and red represents iNOS. In D , green represents CD68+ and red represents CD206+)(_200).

    Article Snippet: Antigen retrieval was performed by cooking tissue sections for 30 min in Tris-EDTA buffer and applying the following primary antibodies: CD68 + (ready-to-use, Maixin, Fuzhou, China), CD3 + (1:1000, Santa Cruz, California, USA), TGFβ1 (1:800, Bioworld, Louis Park, MN, USA), βCGRP (1:800, ABclonal, Wuhan, China), αCGRP (1:800, ABclonal, Wuhan, China), BAX (1:500, proteintech, Chicago, USA), and PPAR-ɤ (1:800, Bioss, Beijing, China) at 4 °C overnight.

    Techniques: Expressing, TUNEL Assay, Staining, Control, Immunofluorescence

    (A) The percentage of CD11c + BMDCs was analyzed by FACS. (B) BMDCs were incubated with or without CGRP in the presence of LPS stimulation. The cellular cAMP levels were determined after the indicated time periods. Values are presented as mean ± SD (n = 3). (C) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 VS. LPS only. (D) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01. (E) The mRNA expression was determined by real-time PCR after 6 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. LPS only. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added at the start of stimulation.

    Journal: PLoS ONE

    Article Title: Calcitonin Gene-Related Peptide Regulates Type IV Hypersensitivity through Dendritic Cell Functions

    doi: 10.1371/journal.pone.0086367

    Figure Lengend Snippet: (A) The percentage of CD11c + BMDCs was analyzed by FACS. (B) BMDCs were incubated with or without CGRP in the presence of LPS stimulation. The cellular cAMP levels were determined after the indicated time periods. Values are presented as mean ± SD (n = 3). (C) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 VS. LPS only. (D) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01. (E) The mRNA expression was determined by real-time PCR after 6 h stimulation with LPS. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. LPS only. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added at the start of stimulation.

    Article Snippet: LPS, PKA inhibitor H89, and phosphodiesterase inhibitor IBMX (3-isobtyl-1-methylxanthine) were purchased from Sigma Aldrich (St. Louis, MO). αCGRP was purchased from Peptide Institute (Osaka, Japan), and βCGRP was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction

    (A) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with OVA and anti-CD40 mAb. Values are presented as means ± SD (n = 3). ** P <0.01 VS. OVA+anti-CD40 mAb. (B) The mRNA expression was determined by real-time PCR after 6 h stimulation with OVA and anti-CD40 mAb. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. OVA+anti-CD40 mAb. (C) BMDCs and DO 11.10 Th0 cells were co-cultured in the presence of 200 µg/ml OVA for 72 h. IFN-γ production was detected by FACS after intracellular staining. Data are represented of three independent experiments. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added from start point of stimulation.

    Journal: PLoS ONE

    Article Title: Calcitonin Gene-Related Peptide Regulates Type IV Hypersensitivity through Dendritic Cell Functions

    doi: 10.1371/journal.pone.0086367

    Figure Lengend Snippet: (A) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with OVA and anti-CD40 mAb. Values are presented as means ± SD (n = 3). ** P <0.01 VS. OVA+anti-CD40 mAb. (B) The mRNA expression was determined by real-time PCR after 6 h stimulation with OVA and anti-CD40 mAb. Values are presented as mean ± SD (n = 3). * P <0.05, ** P <0.01 vs. OVA+anti-CD40 mAb. (C) BMDCs and DO 11.10 Th0 cells were co-cultured in the presence of 200 µg/ml OVA for 72 h. IFN-γ production was detected by FACS after intracellular staining. Data are represented of three independent experiments. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added from start point of stimulation.

    Article Snippet: LPS, PKA inhibitor H89, and phosphodiesterase inhibitor IBMX (3-isobtyl-1-methylxanthine) were purchased from Sigma Aldrich (St. Louis, MO). αCGRP was purchased from Peptide Institute (Osaka, Japan), and βCGRP was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Staining