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primary antibody α2m  (Proteintech)


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    Proteintech primary antibody α2m
    Primary Antibody α2m, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B12m/hif+1%CE%B1+antibody/pmc09032618-187-13-41
    Average 90 stars, based on 1 article reviews
    primary antibody α2m - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Saline:

    Article Title: Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer’s Disease Patients
    Article Snippet: Proteins were transferred to poly(vinylidene fluoride) (GE Healthcare, Chicago, IL, USA) membranes by semi-dry blotting. .. The membranes were blocked in 5% nonfat dry milk in phosphate-buffered saline with Tween-20 (1× PBS, 7.4 pH, 0.2% Tween) and incubated overnight with primary antibodies at 4 °C: β-actin (1:5000, A5441, Sigma-Aldrich, St. Louis, MO, USA) and α2M (1:1000, 13545-1-AP, Proteintech Group Inc., Rosemont, IL, USA). .. The signals were detected with Cy5 conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (1:1000, Jackson Immuno Research Laboratories Europe Ltd., Cambridgeshire, UK) for β-actin (incubated for 2 h, at RT, protected from light); and anti-rabbit horseradish peroxidase-conjugated antibody (1:3000, 130-65-15, BioRad, Hercules, CA, USA) for α2M, using a substrate solution (Pierce SuperSignal West Pico Plus Chemiluminescent substrate, Waltham, MA, USA) for signal enhancement.

    Incubation:

    Article Title: Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer’s Disease Patients
    Article Snippet: Proteins were transferred to poly(vinylidene fluoride) (GE Healthcare, Chicago, IL, USA) membranes by semi-dry blotting. .. The membranes were blocked in 5% nonfat dry milk in phosphate-buffered saline with Tween-20 (1× PBS, 7.4 pH, 0.2% Tween) and incubated overnight with primary antibodies at 4 °C: β-actin (1:5000, A5441, Sigma-Aldrich, St. Louis, MO, USA) and α2M (1:1000, 13545-1-AP, Proteintech Group Inc., Rosemont, IL, USA). .. The signals were detected with Cy5 conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (1:1000, Jackson Immuno Research Laboratories Europe Ltd., Cambridgeshire, UK) for β-actin (incubated for 2 h, at RT, protected from light); and anti-rabbit horseradish peroxidase-conjugated antibody (1:3000, 130-65-15, BioRad, Hercules, CA, USA) for α2M, using a substrate solution (Pierce SuperSignal West Pico Plus Chemiluminescent substrate, Waltham, MA, USA) for signal enhancement.



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    <t>α2M</t> level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)
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    <t>α2M</t> level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)
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    <t>α2M</t> level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)
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    Image Search Results


    α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

    Journal: Journal of Medical Biochemistry

    Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

    doi: 10.5937/jomb0-39557

    Figure Lengend Snippet: α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

    Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

    Techniques: Staining, Enzyme-linked Immunosorbent Assay

    α2M stimulation decreased ROS, MMP13, and ADAMTS4 of severely degenerated NP tissue. We cultured severely degenerated NP tissue in α2M (50, 150, 200 nmol/L) growth medium for 3 days. The protein expression level of α2M was determined by (A) IHC (magnification: 200×) and (B) quantification analysis. (C) Total ROS level of NP tissue. (D) The content of MMP13 and ADAMTS4 was assayed by ELISA. The values are mean ± SD of three independent experiments. (*P<0.05, **P<0.01)

    Journal: Journal of Medical Biochemistry

    Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

    doi: 10.5937/jomb0-39557

    Figure Lengend Snippet: α2M stimulation decreased ROS, MMP13, and ADAMTS4 of severely degenerated NP tissue. We cultured severely degenerated NP tissue in α2M (50, 150, 200 nmol/L) growth medium for 3 days. The protein expression level of α2M was determined by (A) IHC (magnification: 200×) and (B) quantification analysis. (C) Total ROS level of NP tissue. (D) The content of MMP13 and ADAMTS4 was assayed by ELISA. The values are mean ± SD of three independent experiments. (*P<0.05, **P<0.01)

    Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

    Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

    α2M stimulation reversed HOCl-induced oxidative stress of NP cells. NP cells of mildly degenerated NP tissue were treated with HOCl (from 10 to 50 μmol/L) for 6 h, or treated with 30 μmol/L from 3 h to 24 h; Besides, NP cells were pretreated with 30 μmol/L HOCH for 6 h and then cultured with α2M (from 50 to 200 nmol/L) for another 24 h, or cultured with 200 nmol/L α2M from 3 h to 24 h. (A, B) The protein expression level of α2M was determined by ELISA. (C, D) Total ROS level of NP cells. The values are mean ± SD of three independent experiments.

    Journal: Journal of Medical Biochemistry

    Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

    doi: 10.5937/jomb0-39557

    Figure Lengend Snippet: α2M stimulation reversed HOCl-induced oxidative stress of NP cells. NP cells of mildly degenerated NP tissue were treated with HOCl (from 10 to 50 μmol/L) for 6 h, or treated with 30 μmol/L from 3 h to 24 h; Besides, NP cells were pretreated with 30 μmol/L HOCH for 6 h and then cultured with α2M (from 50 to 200 nmol/L) for another 24 h, or cultured with 200 nmol/L α2M from 3 h to 24 h. (A, B) The protein expression level of α2M was determined by ELISA. (C, D) Total ROS level of NP cells. The values are mean ± SD of three independent experiments.

    Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

    Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

    α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

    Journal: Journal of Medical Biochemistry

    Article Title: Antioxidative behavior of a2-macroglobulin in intervertebral disc degeneration

    doi: 10.5937/jomb0-39557

    Figure Lengend Snippet: α2M level decreased in degenerated NP tissue. Representative images of (A) MRI, the yellow arrows indicated the operation section, (B) HE staining (magnification: 200×) (C) IHC targeting α2M of both mildly and severely degenerated NP tissue. (magnification: 200×) (D) Quantification analysis of IHC. NP tissue from the 16 patients was lysed to measure (E) α2M with ELISA methods and (F) total ROS level. The values are mean ± SD of three independent experiments. (***P<0.001)

    Article Snippet: Additionally, α2M from human plasma (Sigma-Aldrich, St. Louis, MO, USA) was used to reverse HOCl.

    Techniques: Staining, Enzyme-linked Immunosorbent Assay