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gene exp olfm4 hs00197437 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp olfm4 hs00197437 m1
    Gene Exp Olfm4 Hs00197437 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+transcriptomic+data+set/Gene+Exp%2E+OLFM4%2C+Hs00197437_m1/us11421277-191-53-6
    Average 91 stars, based on 1 article reviews
    gene exp olfm4 hs00197437 m1 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    other:

    Article Title: Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation
    Article Snippet: Gene Gene Name Gene ID TaqMAN # Accession # ACTB Beta-actin 60 Hs01060665_g1 NM_001101.3 CHGA Chromogranin A 1113 Hs00900375_m1 NM_001275.3 DPPIV Dipeptidylpeptidase 4 1803 Hs00175210_m1 NM_001935.3 LGR5 Leucine Rich Repeat Containing G Protein-Coupled Receptor 5 8549 Hs00969422_m1 NM_001277226.1 LYZ Lysozyme 4069 Hs00426232_m1 NM_000239.2 MUC2 Mucin 2 4583 Hs03005103_g1 NM_002457.2 OLFM4 Olfactomedin 4 10562 Hs00197437_m1 NM_006418.4 VIM Vimentin 7431 Hs00185584 NM_003380.3 Supplemental References McCracken et al., 2011.

    Reverse Transcription:

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNA Cleanup and Concentration Micro Kit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) ( OLFM4 Hs00197437_m1, LGR 5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease.
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNACleanup andConcentrationMicroKit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) (OLFM4 Hs00197437_m1, LGR5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Article Title: Methods and compositions for predicting chronic lung allograft dysfunction
    Article Snippet: .. After reverse transcription with Superscript III (Invitrogen) real-time quantitative PCR was performed on a Taqman StepOne plus real time PCR system (Applied Biosystems) using commercially available primers: HPRT1 (Hs99999909_m1), β2M (Hs00984230_m1), ACTB (Hs99999903_m1), CD19 (Hs99999192_m1), TCL1A (Hs00951350_m1) ELANE (Hs00975994_g1), AZU1 (Hs00156049_m1), FCRL6 (Hs02341772_m1), IGLL5 (Hs04330879_u1), POU2AF1 (Hs01573371_m1), BLK (Hs01017452_m1), DEFA3 (Hs00414018_m1) and OLFM4 (Hs00197437_m1). ..

    Article Title: Methods and compositions for predicting chronic lung allograft dysfunction
    Article Snippet: .. After reverse transcription with Superscript III (Invitrogen) real-time quantitative PCR was performed on a Taqman StepOne plus real time PCR system (Applied Biosystems) using commercially available primers: HPRT1 (Hs99999909_m1), β2M(Hs00984230_m1), ACTB (Hs99999903_m1), DEFA4 (Hs01056651_g1), ELANE (Hs00975994_g1), AZU1 Hs00156049_m1, FCRL6 (Hs02341772_m1), IGLL5 (Hs04330879_u1), POU2AF1 (Hs01573371_m1), CTSL1 (Hs00964650_m1), KRLC3 (Hs01652462_m1), KRLC4 (Hs00255338_m1), BLK (Hs01017452_m1), DEFA3 (Hs00414018_m1) and OLFM4 (Hs00197437_m1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNA Cleanup and Concentration Micro Kit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) ( OLFM4 Hs00197437_m1, LGR 5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease.
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNACleanup andConcentrationMicroKit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) (OLFM4 Hs00197437_m1, LGR5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Article Title: Methods and compositions for predicting chronic lung allograft dysfunction
    Article Snippet: .. After reverse transcription with Superscript III (Invitrogen) real-time quantitative PCR was performed on a Taqman StepOne plus real time PCR system (Applied Biosystems) using commercially available primers: HPRT1 (Hs99999909_m1), β2M (Hs00984230_m1), ACTB (Hs99999903_m1), CD19 (Hs99999192_m1), TCL1A (Hs00951350_m1) ELANE (Hs00975994_g1), AZU1 (Hs00156049_m1), FCRL6 (Hs02341772_m1), IGLL5 (Hs04330879_u1), POU2AF1 (Hs01573371_m1), BLK (Hs01017452_m1), DEFA3 (Hs00414018_m1) and OLFM4 (Hs00197437_m1). ..

    Article Title: Methods and compositions for predicting chronic lung allograft dysfunction
    Article Snippet: .. After reverse transcription with Superscript III (Invitrogen) real-time quantitative PCR was performed on a Taqman StepOne plus real time PCR system (Applied Biosystems) using commercially available primers: HPRT1 (Hs99999909_m1), β2M(Hs00984230_m1), ACTB (Hs99999903_m1), DEFA4 (Hs01056651_g1), ELANE (Hs00975994_g1), AZU1 Hs00156049_m1, FCRL6 (Hs02341772_m1), IGLL5 (Hs04330879_u1), POU2AF1 (Hs01573371_m1), CTSL1 (Hs00964650_m1), KRLC3 (Hs01652462_m1), KRLC4 (Hs00255338_m1), BLK (Hs01017452_m1), DEFA3 (Hs00414018_m1) and OLFM4 (Hs00197437_m1). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNA Cleanup and Concentration Micro Kit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) ( OLFM4 Hs00197437_m1, LGR 5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Article Title: Macrophage and neutrophil heterogeneity at single-cell spatial resolution in human inflammatory bowel disease.
    Article Snippet: Organoid samples were then harvested in Trizol (Ambion, Thermo Fisher Scientific, USA) and RNA isolated using GeneJET RNACleanup andConcentrationMicroKit (Thermo Fisher Scientific, USA). .. Total RNA was transcribed to cDNA using reverse transcriptase (High Capacity cDNA Archive RT kit, Applied Biosystems, Carlsbad, CA, USA) Quantitative real-time PCR (qPCR) was performed in an ABI PRISM 7500 Fast RT-PCR System (Applied Biosystems) using predesigned TaqMan Assays (Applied Biosystems) (OLFM4 Hs00197437_m1, LGR5 Hs00173664_m1 and MKI67 Hs00606991_m1). ..

    Gene Expression:

    Article Title: Modification of Diet to Reduce the Stemness and Tumorigenicity of Murine and Human Intestinal Cells
    Article Snippet: Analysis was performed on the QuantoStudio7 Flex real-time PCR (Applied Biosystems, UK). .. Assay on demand probes used were Lgr5 Mm00438890_m1, Olfm4 Mm01320260_m1, ActB 4352933E, LGR5 Hs00969422_m1, OLFM4 Hs00197437_m1, ASCL2 Hs00270888_s1 and GAPDH Hs02786624_g1) Data were normalised against housekeeping gene expression. ..



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