il 1β (Proteintech)
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Proteintech
il 1β
Il 1β, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+transcriptomic+data+set/IL-1+beta+Antibody/ppr0433194-51-33-51
Average 96 stars, based on 2435 article reviews
Il 1β, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+transcriptomic+data+set/IL-1+beta+Antibody/ppr0433194-51-33-51
Average 96 stars, based on 2435 article reviews
il 1β - by Bioz Stars,
2026-11
96/100 stars
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Incubation:Article Title: Trans-cuff budesonide delivery and its effects on endotracheal intubation-induced subglottic injury Article Snippet: .. For detection of inflammatory cytokines, sections were incubated overnight at 4 °C with Article Title: SOCS1/Wnt/IL-1β axis negatively regulates macrophage extracellular traps and M1 polarization through the TNF pathway in crystalline kidney injury. Article Snippet: Background: Calcium oxalate (CaOx) crystals are known to induce kidney damage and trigger inflammatory responses.. However, the role of macrophage extracellular traps (METs) in CaOx-induced kidney injury and the relationship between METs and the M1 polarization phenotype of macrophages remain unclear.. Methods: A CaOx crystal deposition model was established in C57BL/6J mice using a high-calcium diet supplemented with vitamin D. Renal tissues from patients with CaOx stones and healthy controls were also collected. Article Title: Plasma-derived exosomes in chronic spontaneous urticaria induce the release of inflammatory mediators from mast cell through miRNA-619-5p/SOCS4 pathway. Article Snippet: Background: The excessive release of inflammatory mediators in mast cells is considered a core link in the progression of Chronic spontaneous Urticaria (CSU).. Exosomes are nanosized membrane-bound fluid vesicles in the body that can induce immune dysfunction and impact disease progression.. This study aims to explore the critical role of exosomes derived from plasma of patients in the progression of CSU. Article Title: Integrated transcriptome and single-cell RNA sequencing identifies transient receptor potential channel-related biomarkers in ulcerative colitis Article Snippet: Protein concentrations were measured using a bicinchoninic acid (BCA) assay kit (Solarbio, Beijing, China). .. For Western blot analysis, equal amounts of protein (50 μg) were denatured in SDS loading buffer, separated by SDS-PAGE (80 V for 25 min, then 120 V for 50 min), and transferred to PVDF membranes (Millipore, MA, United States) at 200 mA for 2 h. Membranes were blocked with blocking buffer (NCM Bio, P30500 ) for 10 min at room temperature and subsequently incubated overnight at 4 °C with primary Article Title: SOCS3 Suppression Is Associated with Hippocampal Inflammation in a Mouse Model of Intermittent Hypoxia Article Snippet: .. Then, the membranes were incubated overnight at 4 ◦C with primary antibodies against SOCS3 (1:1000, ab16030, Abcam, Cambridge, MA, USA), TRAF6 (1:4000, 66498-1-Ig, Proteintech, Rosemont, IL, USA), NF-κB-p65 (1:500, ab7970, Abcam, Cambridge, MA, USA), TNF-α (1:1000, ab183218, Abcam, Cambridge, MA, USA), Membrane:Article Title: Plasma-derived exosomes in chronic spontaneous urticaria induce the release of inflammatory mediators from mast cell through miRNA-619-5p/SOCS4 pathway. Article Snippet: Background: The excessive release of inflammatory mediators in mast cells is considered a core link in the progression of Chronic spontaneous Urticaria (CSU).. Exosomes are nanosized membrane-bound fluid vesicles in the body that can induce immune dysfunction and impact disease progression.. This study aims to explore the critical role of exosomes derived from plasma of patients in the progression of CSU. Western Blot:Article Title: Integrated transcriptome and single-cell RNA sequencing identifies transient receptor potential channel-related biomarkers in ulcerative colitis Article Snippet: Protein concentrations were measured using a bicinchoninic acid (BCA) assay kit (Solarbio, Beijing, China). .. For Western blot analysis, equal amounts of protein (50 μg) were denatured in SDS loading buffer, separated by SDS-PAGE (80 V for 25 min, then 120 V for 50 min), and transferred to PVDF membranes (Millipore, MA, United States) at 200 mA for 2 h. Membranes were blocked with blocking buffer (NCM Bio, P30500 ) for 10 min at room temperature and subsequently incubated overnight at 4 °C with primary Analysis:Article Title: Integrated transcriptome and single-cell RNA sequencing identifies transient receptor potential channel-related biomarkers in ulcerative colitis Article Snippet: Protein concentrations were measured using a bicinchoninic acid (BCA) assay kit (Solarbio, Beijing, China). .. For Western blot analysis, equal amounts of protein (50 μg) were denatured in SDS loading buffer, separated by SDS-PAGE (80 V for 25 min, then 120 V for 50 min), and transferred to PVDF membranes (Millipore, MA, United States) at 200 mA for 2 h. Membranes were blocked with blocking buffer (NCM Bio, P30500 ) for 10 min at room temperature and subsequently incubated overnight at 4 °C with primary SDS Page:Article Title: Integrated transcriptome and single-cell RNA sequencing identifies transient receptor potential channel-related biomarkers in ulcerative colitis Article Snippet: Protein concentrations were measured using a bicinchoninic acid (BCA) assay kit (Solarbio, Beijing, China). .. For Western blot analysis, equal amounts of protein (50 μg) were denatured in SDS loading buffer, separated by SDS-PAGE (80 V for 25 min, then 120 V for 50 min), and transferred to PVDF membranes (Millipore, MA, United States) at 200 mA for 2 h. Membranes were blocked with blocking buffer (NCM Bio, P30500 ) for 10 min at room temperature and subsequently incubated overnight at 4 °C with primary Blocking Assay:Article Title: Integrated transcriptome and single-cell RNA sequencing identifies transient receptor potential channel-related biomarkers in ulcerative colitis Article Snippet: Protein concentrations were measured using a bicinchoninic acid (BCA) assay kit (Solarbio, Beijing, China). .. For Western blot analysis, equal amounts of protein (50 μg) were denatured in SDS loading buffer, separated by SDS-PAGE (80 V for 25 min, then 120 V for 50 min), and transferred to PVDF membranes (Millipore, MA, United States) at 200 mA for 2 h. Membranes were blocked with blocking buffer (NCM Bio, P30500 ) for 10 min at room temperature and subsequently incubated overnight at 4 °C with primary Recombinant:Article Title: Theabrownin from Pu-erh Tea Restores Intestinal Immunity via Gut Microbiota-Metabolite Network in Immunosuppressed Mice. Article Snippet: Ripened Pu-erh tea (No.7262, 2018, Taetea Group, Kunming, China), CTX (0.5 g, Hengrui Pharmaceuticals Co., Ltd., Lianyungang, China), levamisole hydrochloride (LH) (Guilin Pharmaceutical Co., Ltd., Guilin, China), and lgG, lgM and lgA enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China) were obtained. .. Primary antibodies, toll-like receptor 2 (TLR2), TLR4, mediated myeloid differentiation factor 88 (MyD88), claudin-1, occludin, tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), other:Article Title: Moringa oleifera Leaf-Derived Exosome-Like Nanoparticles Target Macrophage Senescence to Drive M2 Polarisation and Attenuate Alveolar Bone Loss Article Snippet: For immunohistochemistry, sections underwent antigen retrieval, followed by overnight incubation at 4 °C with primary |