rneasy mini kit (Qiagen)

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Qiagen rneasy mini kit
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+transcriptomic+data+set/RNeasy+Mini+Kit/pmc08773574-60-20-23
Average 99 stars, based on 40647 article reviews

rneasy mini kit - by Bioz Stars, 2026-10

99/100 stars

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Expressing:

Article Title: MMEJ repair of breaks at TA repeats maintains ecDNA and cancer fitness.
Article Snippet: .. RT–qPCR to detect cre recombinase expression Total RNA was isolated using RNeasy Mini Kit (QIAGEN, 74106) following the manufacturer’s instructions. .. After treatment with DNAse I (Ambion, AM2222), 0.5 μg of purified RNA was retro-transcribed with random hexamers by using SuperScript IV First-strand System (Invitrogen), cre recombinase expression was assessed using a 1:20 dilution of cDNA with PowerUp SYBR Green Master Mix (Applied Biosystems) and standard cycling conditions on a QuantStudio 6 Flex real-time PCR system.

Isolation:

Article Title: Decline of chaperone-mediated autophagy in aging impairs macrophage clearance of senescent cells.
Article Snippet: .. RNA was isolated using TRIzol (Thermo Fisher, 15596018) and RNeasy Mini kit (QIAGEN, 74106) following manufacturers’ instructions. .. RNA (1 μg) was reverse transcribed into complementary DNA using QuantiTect Reverse Transcription kit (QIAGEN, 205314).

Article Title: Human norovirus infection does not induce serotonin secretion in human intestinal organoids.
Article Snippet: .. Briefly, total RNA was extracted from each well using either the RNAeasy minikit (#74106, Qiagen) or the Kingfisher flex purification system and Magmax-96 viral RNA isolation kit (#4462359, Applied Biosystems) and used for RT-qPCR. ..

RNA Extraction:

Article Title: Inhibition of heparan sulfate synthesis by resveratrol in mucopolysaccharidosis type I and IIIB
Article Snippet: Following 24 h incubation, cells were homogenized and lysed with a QIAshredder column (Cat#79656, Qiagen, Venlo, The Netherlands), with the use of a solution containing guanidine isothiocyanate and β-mercaptoethanol. .. RNA extraction was performed using the RNeasy Mini kit (Cat74106, Qiagen, Venlo, The Netherlands) and treatedt with Turbo DNase (Cat#AM1907, Invitrogen Thermo Fisher Scientific, Waltham, Massachusetts, USA), according to the manufacturers’ instructions. .. The quality of isolated RNA samples was assessed by NanoDrop.

Lysis:

Article Title: A chimeric murine coronavirus with altered deISGylase and proteolytic activities exhibits attenuated viral pathogenesis.
Article Snippet: .. BMDMs were lysed with 350 μL RLT lysis buffer, and RNA was extracted with the RNeasy Mini kit (Qiagen 74106). .. Next, 500 ng of total RNA was converted to cDNA using the RT2 First Strand Kit (Qiagen 330404), and quantitative PCR (qPCR) was performed using the RT2 SYBR Green qPCR Mastermix (Qiagen 330502).

Purification:

Sequencing:

Article Title: Topoisomerase-Based CDH17 Antibody Drug Conjugates Overcome P-gp Resistance but Undergo Target Mediated Clearance in Colorectal Cancer
Article Snippet: .. Posi ve hybridomas were subcloned and an body sequencing performed using RNA extracted from hybridoma cells (RNeasy Mini kit, Qiagen, 74106, RRID: SCR_008585). .. cDNA was synthesized from these RNAs using High-Capacity cDNA Reverse Transcrip on Kit (ThermoFisher Scien fic, 4368814, RRID:SCR_025219) and sequencing was performed by Laragen Inc.

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