Review



rneasy plus mini kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+transcriptomic+data+set/RNeasy+Plus+Mini+Kit/pmc02722886-458-37-41
    Average 99 stars, based on 5282 article reviews
    rneasy plus mini kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Chimeric antigen receptor T cell targeting CD44E variant in HCC holds therapeutic potential
    Article Snippet: .. Total RNA was isolated using the RNeasy Plus Mini kit (Qiagen, cat. no. 74136) according to the manufacturer’s instructions. .. RNA concentration was quantified using a Nanodrop 2000 Spectrophotometer, and cDNA was synthesized from 1 μg of total RNA using SuperScript III First-Strand Synthesis System (Invitrogen, cat. no. 18080051).

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: The following antibodies were used: mouse anti-E-cadherin (BD Pharmingen, 610182; 1:5000), rabbit anti-EpCAM (abcam, ab32392, 1:1000), rabbit anti-fibronectin (abcam, ab2413, 1:5000), mouse anti-mNeonGreen (ChromoTek, 32F6, 1:1000), mouse anti-N-cadherin (BD Pharmingen, 610920, 1:2000), mouse anti-MRE11 (Novus, NB100-473, 1:1000), rabbit anti-NBS1 (Novus, NB100-143, 1:1000), mouse anti-RAD50 (GeneTex, GTX70228, 1:1000), mouse anti-αSMA (Sigma, A2547, 1:5000), rabbit antivimentin (Cell Signaling, 5741, 1:1000), rabbit anti-ZEB1 (Sigma Prestige, HPA027524; 1:2000), rabbit anti-ZEB1 (Proteintech, 21544-1-AP, 1:5000), mouse anti-β-actin (Sigma, A5441; 1:5000), rabbit anti-β-tubulin (Cell Signaling, 2146, 1:10,000), rabbit anti-GAPDH (Cell Signaling, 2118, 1:20,000), goat anti-mouse IgG HRP (Jackson ImmunoResearch, 115–035–146, 1:10,000), and goat anti-rabbit IgG HRP (Jackson ImmunoResearch, 111– 035–144, 1:10,000). .. Total RNA was isolated and reversely transcribed using the RNeasy Plus Mini kit (Qiagen, 74136) and the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622) as previously described [44]. cDNA was amplified in 384-well plates using gene-specific primers using the Universal Probe Library (Roche, 04869877001) and the TaqMan Universal Master Mix (Applied Biosystems, 4440040). .. Samples were run in triplicates in a LightCycler 480 II (Roche) and normalized to ACTB.

    Transfection:

    Article Title: Premature cleavage and polyadenylation in the minor intron of PTEN modulate its expression and generate a functional long non-coding RNA
    Article Snippet: .. RNA was extracted from cells following transfection or inhibitor treatments using Qiagen’s RNeasy Plus Mini Kit (Catalog #: 74136), according to manufacturer’s recommendations. ..

    cDNA Synthesis:

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: The following antibodies were used: mouse anti-E-cadherin (BD Pharmingen, 610182; 1:5000), rabbit anti-EpCAM (abcam, ab32392, 1:1000), rabbit anti-fibronectin (abcam, ab2413, 1:5000), mouse anti-mNeonGreen (ChromoTek, 32F6, 1:1000), mouse anti-N-cadherin (BD Pharmingen, 610920, 1:2000), mouse anti-MRE11 (Novus, NB100-473, 1:1000), rabbit anti-NBS1 (Novus, NB100-143, 1:1000), mouse anti-RAD50 (GeneTex, GTX70228, 1:1000), mouse anti-αSMA (Sigma, A2547, 1:5000), rabbit antivimentin (Cell Signaling, 5741, 1:1000), rabbit anti-ZEB1 (Sigma Prestige, HPA027524; 1:2000), rabbit anti-ZEB1 (Proteintech, 21544-1-AP, 1:5000), mouse anti-β-actin (Sigma, A5441; 1:5000), rabbit anti-β-tubulin (Cell Signaling, 2146, 1:10,000), rabbit anti-GAPDH (Cell Signaling, 2118, 1:20,000), goat anti-mouse IgG HRP (Jackson ImmunoResearch, 115–035–146, 1:10,000), and goat anti-rabbit IgG HRP (Jackson ImmunoResearch, 111– 035–144, 1:10,000). .. Total RNA was isolated and reversely transcribed using the RNeasy Plus Mini kit (Qiagen, 74136) and the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622) as previously described [44]. cDNA was amplified in 384-well plates using gene-specific primers using the Universal Probe Library (Roche, 04869877001) and the TaqMan Universal Master Mix (Applied Biosystems, 4440040). .. Samples were run in triplicates in a LightCycler 480 II (Roche) and normalized to ACTB.

    Amplification:

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: The following antibodies were used: mouse anti-E-cadherin (BD Pharmingen, 610182; 1:5000), rabbit anti-EpCAM (abcam, ab32392, 1:1000), rabbit anti-fibronectin (abcam, ab2413, 1:5000), mouse anti-mNeonGreen (ChromoTek, 32F6, 1:1000), mouse anti-N-cadherin (BD Pharmingen, 610920, 1:2000), mouse anti-MRE11 (Novus, NB100-473, 1:1000), rabbit anti-NBS1 (Novus, NB100-143, 1:1000), mouse anti-RAD50 (GeneTex, GTX70228, 1:1000), mouse anti-αSMA (Sigma, A2547, 1:5000), rabbit antivimentin (Cell Signaling, 5741, 1:1000), rabbit anti-ZEB1 (Sigma Prestige, HPA027524; 1:2000), rabbit anti-ZEB1 (Proteintech, 21544-1-AP, 1:5000), mouse anti-β-actin (Sigma, A5441; 1:5000), rabbit anti-β-tubulin (Cell Signaling, 2146, 1:10,000), rabbit anti-GAPDH (Cell Signaling, 2118, 1:20,000), goat anti-mouse IgG HRP (Jackson ImmunoResearch, 115–035–146, 1:10,000), and goat anti-rabbit IgG HRP (Jackson ImmunoResearch, 111– 035–144, 1:10,000). .. Total RNA was isolated and reversely transcribed using the RNeasy Plus Mini kit (Qiagen, 74136) and the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1622) as previously described [44]. cDNA was amplified in 384-well plates using gene-specific primers using the Universal Probe Library (Roche, 04869877001) and the TaqMan Universal Master Mix (Applied Biosystems, 4440040). .. Samples were run in triplicates in a LightCycler 480 II (Roche) and normalized to ACTB.

    Purification:

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: .. Total RNA was purified from mouse brain samples and primary mouse microglia obtained from WT or TRPM4KO mouse using the RNeasy Plus Mini kit (Qiagen) with DNase I (Sigma-Aldrich) treatment to eliminate genomic DNA according to the manufacturer’s protocols. cDNA was synthesized from total RNA (up to 1 μg) by reverse transcription using the SuperScript III first-strand synthesis system for RT-PCR (Invitrogen). .. PCR was performed using KOD DNA polymerase (Toyobo) according to the manufacture’s protocol together with specific primer sets.

    Synthesized:

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: .. Total RNA was purified from mouse brain samples and primary mouse microglia obtained from WT or TRPM4KO mouse using the RNeasy Plus Mini kit (Qiagen) with DNase I (Sigma-Aldrich) treatment to eliminate genomic DNA according to the manufacturer’s protocols. cDNA was synthesized from total RNA (up to 1 μg) by reverse transcription using the SuperScript III first-strand synthesis system for RT-PCR (Invitrogen). .. PCR was performed using KOD DNA polymerase (Toyobo) according to the manufacture’s protocol together with specific primer sets.

    Reverse Transcription:

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: .. Total RNA was purified from mouse brain samples and primary mouse microglia obtained from WT or TRPM4KO mouse using the RNeasy Plus Mini kit (Qiagen) with DNase I (Sigma-Aldrich) treatment to eliminate genomic DNA according to the manufacturer’s protocols. cDNA was synthesized from total RNA (up to 1 μg) by reverse transcription using the SuperScript III first-strand synthesis system for RT-PCR (Invitrogen). .. PCR was performed using KOD DNA polymerase (Toyobo) according to the manufacture’s protocol together with specific primer sets.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: .. Total RNA was purified from mouse brain samples and primary mouse microglia obtained from WT or TRPM4KO mouse using the RNeasy Plus Mini kit (Qiagen) with DNase I (Sigma-Aldrich) treatment to eliminate genomic DNA according to the manufacturer’s protocols. cDNA was synthesized from total RNA (up to 1 μg) by reverse transcription using the SuperScript III first-strand synthesis system for RT-PCR (Invitrogen). .. PCR was performed using KOD DNA polymerase (Toyobo) according to the manufacture’s protocol together with specific primer sets.



    Similar Products

    zebrafish transcriptomic data set  (10X Genomics)
    86
    10X Genomics zebrafish transcriptomic data set
    Zebrafish Transcriptomic Data Set, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+transcriptomic+data+set/10x+cancer+data+lung+squamous+visium/pm32694805-201-2-5
    Average 86 stars, based on 1 article reviews
    zebrafish transcriptomic data set - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results