sgrna (New England Biolabs)

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New England Biolabs sgrna
Sgrna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zebrafish+codon+optimized+cas9+coding+sequence/Cas9+Nuclease/pmc08496789-238-37-41
Average 96 stars, based on 1578 article reviews

sgrna - by Bioz Stars, 2026-09

96/100 stars

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Recombinant:

Article Title: Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency
Article Snippet: The concentration of ABE protein in purified eVLPs was quantified using the FastScan TM Cas9 ( S. pyogenes ) ELISA kit (Cell Signaling Technology; 29666 C) according to the manufacturer’s protocols. .. Recombinant Cas9 ( S. pyogenes ) nuclease protein (New England Biolabs; M0386) was used to generate the standard curve for quantification. .. The concentration of MLV p30 protein in purified eVLPs was quantified using the MuLV Core Antigen ELISA kit (Cell Biolabs; VPK-156) according to the manufacturer’s protocols.

Article Title: Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency.
Article Snippet: The concentration of ABE protein in purified eVLPs was quantified using the FastScanTM Cas9 (S. pyogenes) ELISA kit (Cell Signaling Technology; 29666C) according to the manufacturer’s protocols. .. Recombinant Cas9 (S. pyogenes) nuclease protein (New England Biolabs; M0386) was used to generate the standard curve for quantification. .. The concentration of MLV p30 protein in purified eVLPs was quantified using the MuLV Core Antigen ELISA kit (Cell Biolabs; VPK-156) according to the manufacturer’s protocols.

Injection:

Article Title: A reparative neutrophil subpopulation promotes spinal cord regeneration in zebrafish by controlling macrophage inflammation via Il-4
Article Snippet: All gRNAs and tracrRNA were ordered from Merck KGaA (Darmstadt, Germany). .. 1 nL of gRNA mix (1 μL of Tracer (5nM, Merck KGaA, Darmstadt, Germany; Cat#TRACRRNA05N), 1 μL of each gRNA (20μM,Merck KGaA, Darmstadt, Germany), 1 μL of phenol red (Sigma-Aldrich, St. Louis, MO, USA; Cat#P0290) and 1 μL of Cas9 (20μM, New England Biolabs, Ipswich, MA, USA; CatM0386M) was injected into one-cell stage embryos. gRNA efficiency was tested by restriction fragment length polymorphism analysis (RFLP) in a proportion of larvae for each experiment as an internal experimental control ( Keatinge et al ., 2021 ). .. Sequences for gRNAs, primers and the restriction enzymes (RE, New England Biolabs, Ipswich, MA, USA) used for RFLPs are provided in .

Control:

CRISPR:

Article Title: Benchmarking of sequencing technologies defines optimal strategies for genetic variants detection in a human genome
Article Snippet: The DNA extractions for HG002 and HG004 were prepared following the SageHLS High Molecular Weight DNA Extraction protocol using a 1/800 dilution of NEBNext® dsDNA Fragmentase® (cat# M0348) for a half hour digestion. .. DNA from HG003 was prep testing an alternative protocol which uses a Cas9 system (Cas9 Nuclease, S. pyogenes , New England BioLabs, Inc., cat# M0386) with two simple guide RNAs (a 10 CA repeat guide and a 10 AT repeat guide, Alt-R® CRISPR-Cas9 crRNA, Integrated DNA Technologies, Inc., Coralville, Iowa, United States). .. DNA yield from each elution well was measured by QubitTM dsDNA BR Assay Kit (ThermoFisher Scientific, cat# Q32853 ) and molecule length was assessed by Femto Pulse (Genomic DNA 165 kb Kit, 3 h run, Agilent Technologies, Inc., Santa Clara, California, United States, cat# FP-1002–0275).

Article Title: Benchmarking of sequencing technologies defines optimal strategies for genetic variants detection in a human genome.
Article Snippet: The DNA extractions for HG002 and HG004 were prepared following the SageHLS High Molecular Weight DNA Extraction protocol using a 1/800 dilution of NEBNext® dsDNA Fragmentase® (cat# M0348) for a half hour digestion. .. DNA from HG003 was prep testing an alternative protocol which uses a Cas9 system (Cas9 Nuclease, S. pyogenes, New England BioLabs, Inc., cat# M0386) with two simple guide RNAs (a 10 CA repeat guide and a 10 AT repeat guide, Alt-R® CRISPR-Cas9 crRNA, Integrated DNA Technologies, Inc., Coralville, Iowa, United States). .. DNA yield from each elution well was measured by QubitTM dsDNA BR Assay Kit (ThermoFisher Scientific, cat# Q32853) and molecule length was assessed by Femto Pulse (Genomic DNA 165 kb Kit, 3 hour run, Agilent Technologies, Inc., Santa Clara, California, United States, cat# FP-1002-0275).

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