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cas9 mrna  (New England Biolabs)


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    Structured Review

    New England Biolabs cas9 mrna
    Cas9 Mrna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6897 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zebrafish+codon+optimized+cas9+coding+sequence/XbaI/pmc06405052-214-0-6
    Average 99 stars, based on 6897 article reviews
    cas9 mrna - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sterility:

    Article Title: Risk factors for in vivo emergence of ceftazidime-avibactam resistance in KPC-producing Klebsiella pneumoniae and the associated resistance mechanisms: a case-control study.
    Article Snippet: .. The plugs were then washed with sterile water and TE buffer at 56°C to remove proteinase K. Following digestion of plugs with the restriction endonuclease XbaI (New England Biolabs) at 37°C for 2 h, the plugs were loaded into 1% SKG agarose gel in 0.5× TBE buffer. .. Chromosomal macro-restriction fragment patterns were characterized by electrophoresis conditions that consisted of an initial switch time of 3 s and a final switch time of 35 s at an included angle of 120° and a gradient of 6 V/cm for 24 h with a linear ramp in 0.5× TBE at 14°C on a CHEF Mapper system (Bio-Rad Laboratories).

    Agarose Gel Electrophoresis:

    Article Title: Risk factors for in vivo emergence of ceftazidime-avibactam resistance in KPC-producing Klebsiella pneumoniae and the associated resistance mechanisms: a case-control study.
    Article Snippet: .. The plugs were then washed with sterile water and TE buffer at 56°C to remove proteinase K. Following digestion of plugs with the restriction endonuclease XbaI (New England Biolabs) at 37°C for 2 h, the plugs were loaded into 1% SKG agarose gel in 0.5× TBE buffer. .. Chromosomal macro-restriction fragment patterns were characterized by electrophoresis conditions that consisted of an initial switch time of 3 s and a final switch time of 35 s at an included angle of 120° and a gradient of 6 V/cm for 24 h with a linear ramp in 0.5× TBE at 14°C on a CHEF Mapper system (Bio-Rad Laboratories).

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: The genomic regions identified by bioinformatic analysis (see Section 4.1) were amplified via high-fidelity PCR using the PhusionTM High-Fidelity DNA Polymerase (cat F530S, Thermo Fisher Scientific, Waltham, MA, USA) using sequence-specific primers (see Section 4.14), including restriction sites for XbaI and SalI enzymes at their 5′-ends. .. The PCR product, extracted from the agarose gel using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), and the RHCglo vector was enzymatically cut using XbaI (cat R0145S, New England Biolabs, Ipswich, MA, USA) and SalI High Fidelity® (cat R3138S, New England Biolabs, Ipswich, MA, USA) enzymes, according to the manufacturer’s instructions. .. The digested vector and PCR products were purified using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), ligated using T4 DNA ligase (cat EL0014, Thermo Fisher Scientific, Waltham, MA, USA), and then transformed into chemically competent JM109 E. coli (cat L2005, Promega Corporation, Madison, WI, USA).

    Construct:

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii
    Article Snippet: .. To construct reporter plasmids (pLPV1Z-P pbp1a and pLPV1Z-P isc operon ), promoter regions of the pbp1a operon and the isc operon, respectively, were amplified using the primers listed in Table S2 ( ). pLPV1Z was digested with PstI and XbaI (New England Biolabs). ..

    Amplification:

    Article Title: IscR-mediated morphological regulation confers virulence and stress resistance by reducing stress molecule uptake in Acinetobacter baumannii
    Article Snippet: .. To construct reporter plasmids (pLPV1Z-P pbp1a and pLPV1Z-P isc operon ), promoter regions of the pbp1a operon and the isc operon, respectively, were amplified using the primers listed in Table S2 ( ). pLPV1Z was digested with PstI and XbaI (New England Biolabs). ..

    Article Title: CLASHub is an integrated database and analytical platform for microRNA-target interactions.
    Article Snippet: .. The 3ʹ UTR fragments of human FAM3C, ZBTB4, and ZNF367 containing predicted miR-18a-5p binding sites were amplified and cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector using the NEBuilder HiFi DNA Assembly method (linearized with SacI and XbaI). ..

    other:

    Article Title: Cannabinoid CB 2 receptor drives trastuzumab resistance and predicts durable anti-HER2 response.
    Article Snippet: DNA encoding HER2 (Uni Prot ID:P04626) was from Addgene (plasmid #16257).

    Binding Assay:

    Article Title: CLASHub is an integrated database and analytical platform for microRNA-target interactions.
    Article Snippet: .. The 3ʹ UTR fragments of human FAM3C, ZBTB4, and ZNF367 containing predicted miR-18a-5p binding sites were amplified and cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector using the NEBuilder HiFi DNA Assembly method (linearized with SacI and XbaI). ..

    Clone Assay:

    Article Title: CLASHub is an integrated database and analytical platform for microRNA-target interactions.
    Article Snippet: .. The 3ʹ UTR fragments of human FAM3C, ZBTB4, and ZNF367 containing predicted miR-18a-5p binding sites were amplified and cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector using the NEBuilder HiFi DNA Assembly method (linearized with SacI and XbaI). ..

    Expressing:

    Article Title: CLASHub is an integrated database and analytical platform for microRNA-target interactions.
    Article Snippet: .. The 3ʹ UTR fragments of human FAM3C, ZBTB4, and ZNF367 containing predicted miR-18a-5p binding sites were amplified and cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector using the NEBuilder HiFi DNA Assembly method (linearized with SacI and XbaI). ..

    Ligation:

    Article Title: Magnetogenetic control of endogenous calcium signaling via ROS-sensitive ion channels reveals astrocytic regulation of neural circuit activity
    Article Snippet: PCR amplification of lck–D5–mScarlet-I with 5’ EcoRI and 3’ XbaI restriction sites were performed with SuperFi II (Invitrogen 12349050). .. After restriction digest with EcoRI (NEB R3101) and XbaI (NEB R0145), ligation was performed with T4 DNA ligase (NEB M0202) and transformed with NEB Stable competent E.coli (NEB C3040). .. After cultures were incubated at 30°C for ∼20 hours, endotoxin-free DNA preps were performed with ZymoPURE II Plasmid Maxiprep Kit (Zymo Research D4202).

    Transformation Assay:

    Article Title: Magnetogenetic control of endogenous calcium signaling via ROS-sensitive ion channels reveals astrocytic regulation of neural circuit activity
    Article Snippet: PCR amplification of lck–D5–mScarlet-I with 5’ EcoRI and 3’ XbaI restriction sites were performed with SuperFi II (Invitrogen 12349050). .. After restriction digest with EcoRI (NEB R3101) and XbaI (NEB R0145), ligation was performed with T4 DNA ligase (NEB M0202) and transformed with NEB Stable competent E.coli (NEB C3040). .. After cultures were incubated at 30°C for ∼20 hours, endotoxin-free DNA preps were performed with ZymoPURE II Plasmid Maxiprep Kit (Zymo Research D4202).

    Polymerase Chain Reaction:

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: The genomic regions identified by bioinformatic analysis (see Section 4.1) were amplified via high-fidelity PCR using the PhusionTM High-Fidelity DNA Polymerase (cat F530S, Thermo Fisher Scientific, Waltham, MA, USA) using sequence-specific primers (see Section 4.14), including restriction sites for XbaI and SalI enzymes at their 5′-ends. .. The PCR product, extracted from the agarose gel using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), and the RHCglo vector was enzymatically cut using XbaI (cat R0145S, New England Biolabs, Ipswich, MA, USA) and SalI High Fidelity® (cat R3138S, New England Biolabs, Ipswich, MA, USA) enzymes, according to the manufacturer’s instructions. .. The digested vector and PCR products were purified using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), ligated using T4 DNA ligase (cat EL0014, Thermo Fisher Scientific, Waltham, MA, USA), and then transformed into chemically competent JM109 E. coli (cat L2005, Promega Corporation, Madison, WI, USA).

    Gel Extraction:

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: The genomic regions identified by bioinformatic analysis (see Section 4.1) were amplified via high-fidelity PCR using the PhusionTM High-Fidelity DNA Polymerase (cat F530S, Thermo Fisher Scientific, Waltham, MA, USA) using sequence-specific primers (see Section 4.14), including restriction sites for XbaI and SalI enzymes at their 5′-ends. .. The PCR product, extracted from the agarose gel using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), and the RHCglo vector was enzymatically cut using XbaI (cat R0145S, New England Biolabs, Ipswich, MA, USA) and SalI High Fidelity® (cat R3138S, New England Biolabs, Ipswich, MA, USA) enzymes, according to the manufacturer’s instructions. .. The digested vector and PCR products were purified using the QIAquick Gel Extraction Kit (cat 28704, Qiagen, Venlo, The Netherlands), ligated using T4 DNA ligase (cat EL0014, Thermo Fisher Scientific, Waltham, MA, USA), and then transformed into chemically competent JM109 E. coli (cat L2005, Promega Corporation, Madison, WI, USA).



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