Review




Structured Review

Becton Dickinson zdevd-fmk
Zdevd Fmk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/z+devd+fmk+zdevd/devd+fmk/pm23709189-68-14-18
Average 90 stars, based on 1 article reviews
zdevd-fmk - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Recombinant:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Sterility:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Western Blot:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Expressing:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Incubation:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Staining:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

SDS Page:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

In Vitro:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Knock-Out:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Cytotoxicity Assay:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Software:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Microscopy:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Transfection:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

CCK-8 Assay:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).

Concentration Assay:

Article Title: Colorectal Carcinogenesis: Connecting K-RAS–Induced Transformation and CREB Activity In Vitro and In Vivo
Article Snippet: Cells treated with the caspase-3 inhibitor ZDEVD-FMK (BD) for 24 h served as a control.

Article Title: Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway
Article Snippet: Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform.. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content.. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis.

Article Title: Ring-substituted analogs of 3,3'-diindolylmethane (DIM) induce apoptosis and necrosis in androgen-dependent and -independent prostate cancer cells.
Article Snippet: Control cells were incubated in 0.1 % or 0.2 % DMSO only, where appropriate. zDEVD-fmk, z-IETD-fmk or z-LEHD-fmk (BD Biosciences, Franklin Lakes, NJ) were dissolved in 100 % DMSO to produce 10 mM stock solutions and added to cell cultures 4 h prior to treatment with either ring-DIMs or DMSO vehicle, resulting in a final concentration of 5 μM in culture medium containing 0.2 % DMSO.

Article Title: Caspase-8 inhibition represses initial human monocyte activation in septic shock model
Article Snippet: DEVD-fmk was obtained from Becton Dickinson Bioscience (San Jose, CA, USA).



Article Title: Gasdermin E suppresses tumor growth by activating anti-tumor immunity
Article Snippet: TRAIL was from Enzo Life Sciences. zVAD-fmk and zDEVD-fmk were from BD Biosciences.

Article Title: Caspase-9 mediates Puma activation in UCN-01-induced apoptosis
Article Snippet: UCN-01, PI, the proteasome inhibitor MG132 and CP were obtained from Sigma (St. Louis, MO, USA). zDEVD-fmk and zLEHD-fmk were from BD Biosciences (San Jose, CA, USA).



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MedChemExpress zdevd fmk
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Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
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Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
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Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
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Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
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Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or <t>ZDEVD,</t> were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).
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Image Search Results


Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).

Journal: Acta Dermato-Venereologica

Article Title: Possible Clinical Effects of Ketoconazole on Sorafenib-induced Hand–Foot Skin Reaction and Cytoprotection Mechanisms of Antifungal Agents against Multikinase Inhibitor-induced Keratinocyte Toxicity

doi: 10.2340/actadv.v105.40697

Figure Lengend Snippet: Detection of apoptosis-related factors in NHEK cell lysates following sorafenib and antifungal drug treatment. Detection of (A) cIAP-1 (70 kDa) and (C) cleaved caspase-3 (19 kDa) in the cell lysate was performed using western blotting. (B) Bands were semi-quantified using ImageJ, and data were expressed as the relative ratio of cIAP-1/β-actin. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s test; ns indicates not significant. Sora or S: sorafenib, Keto: ketoconazole, Itra: itraconazole. All results are presented as the mean ± SD of triplicate experiments. (D) Various concentrations of sorafenib, with or without 50 μmM ZVAD or ZDEVD, were added to cultured NHEK cells. DMSO was used as the solvent for both sorafenib and the inhibitors. After overnight incubation, cell viability was assessed using the cell-counting kit-8 assay. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s test; ** p < 0.01 and * p < 0.05 (vs DMSO (-)).

Article Snippet: Z-DEVD-FMK (ZDEVD) and Z-VAD-FMK (ZVAD) were from Selleck Chemicals (Houston, TX, USA).

Techniques: Western Blot, Cell Culture, Solvent, Incubation, Cell Counting