ythdf3 mutants (Addgene inc)
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ythdf3 mutants
Ythdf3 Mutants, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf3/YTHDF3+(Plasmid+%23156133)/pm33378672-236-6-42
Average 93 stars, based on 4 article reviews
Ythdf3 Mutants, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf3/YTHDF3+(Plasmid+%23156133)/pm33378672-236-6-42
Average 93 stars, based on 4 article reviews
ythdf3 mutants - by Bioz Stars,
2026-10
93/100 stars
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Recombinant:Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-Ythdf1 this manuscript Ynzyme custom order rabbit anti-Ythdf2 this manuscript Ynzyme custom order rabbit anti-Ythdf3 this manuscript Ynzyme custom order rabbit anti-Actin Abcam Cat# ab8227; RRID: AB_2305186 goat anti-rabbit IgG, (H+L) HRP conjugate Millipore Cat# AP307P; RRID: AB_92641 Chemicals, Peptides, and Recombinant Proteins triptolide Sigma-aldrich Cat# T3652 a-amanitin Sigma-aldrich Cat# A2263 low melt agarose AmericanBio Cat# CAS: 9012-36-6 N6-methyladenosine 5’-triphosphate TriLink Cat# N-1013-5 m7G(50)ppp(50)G RNA cap structure analog New England BioLabs Cat# S1404S 17 a-ethynylestradiol (EE2) Sigma-Aldrich Cat# E4876 Critical Commercial Assays AmpliScribe-T7-Flash Transcription kit Epicenter Cat# ASF3257 HiScribe SP6 RNA Synthesis Kit New England BioLabs Cat# E2070S mMessage mMachine SP6 Transcription Kit Invitrogen Cat# 74104 Nick Translation Kit Sigma-Aldrich Cat# 10976776001 Poly(A) tailing kit Invitrogen Cat# AM1350 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4368706 Protein A Dynabeads Invitrogen Cat# 10008D RNeasy RNA extraction kit QIAGEN Cat# AM1340 Superscript III Reverse Transcriptase kit Invitrogen Cat# 18080093 TRIzol reagent Invitrogen Cat# 15596-018 Deposited Data mRNA-sequencing of MZythdf2, MZythdf2;MZythdf3, or MZdicer mutants this manuscript SRP297464 mRNA-sequencing of MZythdf2 mutants Zhao et al., 2017 GSE79213 mRNA-sequencing time course of zebrafish embryos Vejnar et al., 2019 Beaudoin et al., 2018 Bazzini et al., 2016 SRP189512 SRP149556 SRP072296 PAL-sequencing in zebrafish embryos Subtelny et al., 2014 GSE52809 TAIL-sequencing in zebrafish embryos Chang et al., 2018 https://doi.org/10.5281/zenodo.2640028 Experimental Models: Organisms/Strains Zebrafish: TU-AB and TLF strains Zebrafish International Resource Center N/A Zebrafish: miR-430 cluster deletion mutant Liu et al., 2013 N/A Zebrafish: ythdf2 8bp mutants Zhao et al., 2017 N/A Zebrafish: dicer mutants Giraldez et al., 2006 N/A Zebrafish: mettl3 mutants this manuscript N/A Zebrafish: mettl14 mutants this manuscript N/A Zebrafish: ythdf1 mutants this manuscript N/A Zebrafish: ythdf2 223bp mutants this manuscript N/A (Continued on next page) Cell Reports 33, 108598, December 29, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zebrafish: Software:Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rabbit anti-Ythdf1 this manuscript Ynzyme custom order rabbit anti-Ythdf2 this manuscript Ynzyme custom order rabbit anti-Ythdf3 this manuscript Ynzyme custom order rabbit anti-Actin Abcam Cat# ab8227; RRID: AB_2305186 goat anti-rabbit IgG, (H+L) HRP conjugate Millipore Cat# AP307P; RRID: AB_92641 Chemicals, Peptides, and Recombinant Proteins triptolide Sigma-aldrich Cat# T3652 a-amanitin Sigma-aldrich Cat# A2263 low melt agarose AmericanBio Cat# CAS: 9012-36-6 N6-methyladenosine 5’-triphosphate TriLink Cat# N-1013-5 m7G(50)ppp(50)G RNA cap structure analog New England BioLabs Cat# S1404S 17 a-ethynylestradiol (EE2) Sigma-Aldrich Cat# E4876 Critical Commercial Assays AmpliScribe-T7-Flash Transcription kit Epicenter Cat# ASF3257 HiScribe SP6 RNA Synthesis Kit New England BioLabs Cat# E2070S mMessage mMachine SP6 Transcription Kit Invitrogen Cat# 74104 Nick Translation Kit Sigma-Aldrich Cat# 10976776001 Poly(A) tailing kit Invitrogen Cat# AM1350 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4368706 Protein A Dynabeads Invitrogen Cat# 10008D RNeasy RNA extraction kit QIAGEN Cat# AM1340 Superscript III Reverse Transcriptase kit Invitrogen Cat# 18080093 TRIzol reagent Invitrogen Cat# 15596-018 Deposited Data mRNA-sequencing of MZythdf2, MZythdf2;MZythdf3, or MZdicer mutants this manuscript SRP297464 mRNA-sequencing of MZythdf2 mutants Zhao et al., 2017 GSE79213 mRNA-sequencing time course of zebrafish embryos Vejnar et al., 2019 Beaudoin et al., 2018 Bazzini et al., 2016 SRP189512 SRP149556 SRP072296 PAL-sequencing in zebrafish embryos Subtelny et al., 2014 GSE52809 TAIL-sequencing in zebrafish embryos Chang et al., 2018 https://doi.org/10.5281/zenodo.2640028 Experimental Models: Organisms/Strains Zebrafish: TU-AB and TLF strains Zebrafish International Resource Center N/A Zebrafish: miR-430 cluster deletion mutant Liu et al., 2013 N/A Zebrafish: ythdf2 8bp mutants Zhao et al., 2017 N/A Zebrafish: dicer mutants Giraldez et al., 2006 N/A Zebrafish: mettl3 mutants this manuscript N/A Zebrafish: mettl14 mutants this manuscript N/A Zebrafish: ythdf1 mutants this manuscript N/A Zebrafish: ythdf2 223bp mutants this manuscript N/A (Continued on next page) Cell Reports 33, 108598, December 29, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Zebrafish: shRNA:Article Title: Autophagy induction promoted by m 6 A reader YTHDF3 through translation upregulation of FOXO3 mRNA Article Snippet: The lentiviral pLV-EGFP-LC3B and pLV-mCherry-EGFP-LC3B plasmids were generated by subcloning EGFP-LC3B fragments (from pEGFP-LC3B, Addgene, #24920) and mCherry-EGFP-LC3B fragments (from pmCherry-EGFP-LC3B) into pCDH-EF1-Neo lentiviral vectors (System Biosciences, Palo Alto, CA, #CD533A-2). .. Lentiviral short-hairpin RNA (shRNA) constructs for Construct:Article Title: Autophagy induction promoted by m 6 A reader YTHDF3 through translation upregulation of FOXO3 mRNA Article Snippet: The lentiviral pLV-EGFP-LC3B and pLV-mCherry-EGFP-LC3B plasmids were generated by subcloning EGFP-LC3B fragments (from pEGFP-LC3B, Addgene, #24920) and mCherry-EGFP-LC3B fragments (from pmCherry-EGFP-LC3B) into pCDH-EF1-Neo lentiviral vectors (System Biosciences, Palo Alto, CA, #CD533A-2). .. Lentiviral short-hairpin RNA (shRNA) constructs for Knock-Out:Article Title: Context-dependent functional compensation between Ythdf m 6 A reader proteins Article Snippet: Naïve conditions for murine ESCs included 10 μg of recombinant human LIF (Peprotech), 3 μM small molecule inhibitors CHIR99021 (CH; Axon Medchem), and 1 μM PD0325901 (PD; Axon Medchem) termed 2i. .. Generation of Ythdf1, Ythdf2, and CRISPR:Article Title: Context-dependent functional compensation between Ythdf m 6 A reader proteins Article Snippet: Naïve conditions for murine ESCs included 10 μg of recombinant human LIF (Peprotech), 3 μM small molecule inhibitors CHIR99021 (CH; Axon Medchem), and 1 μM PD0325901 (PD; Axon Medchem) termed 2i. .. Generation of Ythdf1, Ythdf2, and Clone Assay:Article Title: Context-dependent functional compensation between Ythdf m 6 A reader proteins Article Snippet: Naïve conditions for murine ESCs included 10 μg of recombinant human LIF (Peprotech), 3 μM small molecule inhibitors CHIR99021 (CH; Axon Medchem), and 1 μM PD0325901 (PD; Axon Medchem) termed 2i. .. Generation of Ythdf1, Ythdf2, and Plasmid Preparation:Article Title: Context-dependent functional compensation between Ythdf m 6 A reader proteins Article Snippet: Naïve conditions for murine ESCs included 10 μg of recombinant human LIF (Peprotech), 3 μM small molecule inhibitors CHIR99021 (CH; Axon Medchem), and 1 μM PD0325901 (PD; Axon Medchem) termed 2i. .. Generation of Ythdf1, Ythdf2, and Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development Article Snippet: After PCR amplification, products were purified using QIAquick PCR purification kit (QIAGEN, 28104). sgRNAs were synthesized from the purified PCR product by T7 IVT using an AmpliScribe-T7-Flash Transcription kit (Epicenter, ASF3257), reaction at 37°C for 6 h). sgRNAs were DNase-treated, purified by sodium acetate and ethanol precipitation, and checked for RNA integrity by agarose gel electrophoresis. .. For gene editing to generate ythdf2 −223/−223 and Synthesized:Article Title: Ythdf m 6 A Readers Function Redundantly during Zebrafish Development Article Snippet: After PCR amplification, products were purified using QIAquick PCR purification kit (QIAGEN, 28104). sgRNAs were synthesized from the purified PCR product by T7 IVT using an AmpliScribe-T7-Flash Transcription kit (Epicenter, ASF3257), reaction at 37°C for 6 h). sgRNAs were DNase-treated, purified by sodium acetate and ethanol precipitation, and checked for RNA integrity by agarose gel electrophoresis. .. For gene editing to generate ythdf2 −223/−223 and Transfection:Article Title: CircMIB2 therapy can effectively treat pathogenic infection by encoding a novel protein. Article Snippet: .. The MS2RNA pulldown assay was conducted in MSpC cells transfected with YTHDF1, |
