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ythdf2  (Biorbyt)


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    Biorbyt ythdf2
    Summary of the analyzed m6A proteins as indicated and their correlation with overall survival (indicated as %alive) for the entire study cohort, HPV-independent, and HPV-dependent VSCC. The HPV-status was not available for 24 patients. Samples were grouped according to high and low expression based on the staining intensities. p -values for the group comparisons are based on log-rank tests (significance threshold p < 0.5). q -values are based on multiple hypotheses testing using the method of Benjamini and Hochberg with a significance threshold of q < 0.1
    Ythdf2, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf2/YTHDF2+antibody/pmc09434921-87-98-100
    Average 93 stars, based on 2 article reviews
    ythdf2 - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma"

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma

    Journal: BMC Cancer

    doi: 10.1186/s12885-022-10010-x

    Summary of the analyzed m6A proteins as indicated and their correlation with overall survival (indicated as %alive) for the entire study cohort, HPV-independent, and HPV-dependent VSCC. The HPV-status was not available for 24 patients. Samples were grouped according to high and low expression based on the staining intensities. p -values for the group comparisons are based on log-rank tests (significance threshold p < 0.5). q -values are based on multiple hypotheses testing using the method of Benjamini and Hochberg with a significance threshold of q < 0.1
    Figure Legend Snippet: Summary of the analyzed m6A proteins as indicated and their correlation with overall survival (indicated as %alive) for the entire study cohort, HPV-independent, and HPV-dependent VSCC. The HPV-status was not available for 24 patients. Samples were grouped according to high and low expression based on the staining intensities. p -values for the group comparisons are based on log-rank tests (significance threshold p < 0.5). q -values are based on multiple hypotheses testing using the method of Benjamini and Hochberg with a significance threshold of q < 0.1

    Techniques Used: Expressing, Staining

    Related Articles

    Immunohistochemistry:

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunohistochemistry Page 4/13 Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed depara nization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4°C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Immunostaining:

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunohistochemistry Page 4/13 Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed depara nization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4°C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma.
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Staining:

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunohistochemistry Page 4/13 Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed depara nization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4°C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma.
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Incubation:

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunohistochemistry Page 4/13 Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed depara nization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4°C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma.
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on the TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).

    Article Title: Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
    Article Snippet: .. Immunostaining of METTL3, METTL4, METTL14, WTAP, KIAA1429, FTO, ALKBH5, HNRNPA2B1, HNRNPC, YTHDC1, YTHDF1,YTHDF2, and YTHDF3 was performed on TMAs using an automated staining system (BenchMark ULTRA; Ventana Medical Systems) which performed deparaffinization, pretreatment with cell conditioning buffer (CC1 buffer, pH8), and incubation with primary antibodies (FTO (1:50; Atlas Antibodies #HPA041086), ALKBH5 (1:200; Novus #NBP1-82,188), METTL3 (1:1000; Biorbyt #orb374082), METTL4 (1:40; Atlas Antibodies #HPA040061), METTL14 (1:100; Atlas Antibodies #HPA038002), WTAP (1:100; Atlas Antibodies #HPA010550), KIAA1429 (1:25; Atlas Antibodies #HPA031530), HNRNPC (1:25; Atlas Antibodies #HPA051075), HNRNPA2B1 (1:100; Atlas Antibodies #HPA001666), YTHDC1 (1:25; Atlas Antibodies #HPA036462), YTHDF1 (1:10; Biorbyt #orb179018), YTHDF2 (1:200; Biorbyt #orb39199), YTHDF3 (1:200; Biorbyt #orb374095) at 4 °C overnight. .. Signal detection was performed with the UltraView DAB IHC Detection Kit (Ventana).



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    MedChemExpress ythdf2
    Role of m 6 A in adipogenesis. Insufficient adipogenesis in adipose tissue leads to persistent, chronic inflammation. m 6 A modification plays a crucial role in all stages of adipogenesis, from commitment to terminal differentiation. During commitment, METTL3 promotes lipogenic differentiation in BMSCs by regulating the m 6 A levels of PTH1R and JAK1, whereas silencing METTL14 reduces the expression of SMAD1, inhibiting BMSC proliferation. During terminal differentiation, m 6 A regulates MCE and the transition to mature adipocytes. FTO influences key genes such as ATG5, ATG7 and JAK2, affecting autophagy, STAT3 phosphorylation and adipogenesis. FTO knockout increases the m 6 A levels of CCND1 and CDK2, blocking MCE. m 6 A, N6-methyladenine; METTL, methyltransferase-like; PTH1R, parathyroid hormone 1 receptor; JAK, Janus kinase; BMSC, bone marrow mesenchymal stem cell; MCE, mitotic clone amplification; FTO, Fat mass and obesity-associated protein; ATG, autophagy-related; STAT3, signal transducer and activator of transcription 3; CCND1, cyclin D1; CDK2, cyclin-dependent kinase 2; IGF2BP1, insulin-like growth factor 2 mRNA-binding protein 1; <t>YTHDF2,</t> YTH domain family 2.
    Ythdf2, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf2/YTHDF2%2C+Human/pmc13007997-102-48-54
    Average 91 stars, based on 1 article reviews
    ythdf2 - by Bioz Stars, 2026-10
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    189 ythdf2  (Proteintech)
    96
    Proteintech 189 ythdf2
    Role of m 6 A in adipogenesis. Insufficient adipogenesis in adipose tissue leads to persistent, chronic inflammation. m 6 A modification plays a crucial role in all stages of adipogenesis, from commitment to terminal differentiation. During commitment, METTL3 promotes lipogenic differentiation in BMSCs by regulating the m 6 A levels of PTH1R and JAK1, whereas silencing METTL14 reduces the expression of SMAD1, inhibiting BMSC proliferation. During terminal differentiation, m 6 A regulates MCE and the transition to mature adipocytes. FTO influences key genes such as ATG5, ATG7 and JAK2, affecting autophagy, STAT3 phosphorylation and adipogenesis. FTO knockout increases the m 6 A levels of CCND1 and CDK2, blocking MCE. m 6 A, N6-methyladenine; METTL, methyltransferase-like; PTH1R, parathyroid hormone 1 receptor; JAK, Janus kinase; BMSC, bone marrow mesenchymal stem cell; MCE, mitotic clone amplification; FTO, Fat mass and obesity-associated protein; ATG, autophagy-related; STAT3, signal transducer and activator of transcription 3; CCND1, cyclin D1; CDK2, cyclin-dependent kinase 2; IGF2BP1, insulin-like growth factor 2 mRNA-binding protein 1; <t>YTHDF2,</t> YTH domain family 2.
    189 Ythdf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf2/YTHDF2+Antibody/pm41917949-100-13-16
    Average 96 stars, based on 1 article reviews
    189 ythdf2 - by Bioz Stars, 2026-10
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    List of antibodies used for this study

    Journal: Experimental Hematology & Oncology

    Article Title: Tumor-intrinsic NSUN2 orchestrates immunosuppression in lung adenocarcinoma via the m 5 C-HDAC8-CCL5 axis

    doi: 10.1186/s40164-026-00826-9

    Figure Lengend Snippet: List of antibodies used for this study

    Article Snippet: A25318 , Anti-YTHDF2 , ABclonal.

    Techniques: Control

    The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Coagulation

    Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Clinical Proteomics

    Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing

    Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing

    Role of m 6 A in adipogenesis. Insufficient adipogenesis in adipose tissue leads to persistent, chronic inflammation. m 6 A modification plays a crucial role in all stages of adipogenesis, from commitment to terminal differentiation. During commitment, METTL3 promotes lipogenic differentiation in BMSCs by regulating the m 6 A levels of PTH1R and JAK1, whereas silencing METTL14 reduces the expression of SMAD1, inhibiting BMSC proliferation. During terminal differentiation, m 6 A regulates MCE and the transition to mature adipocytes. FTO influences key genes such as ATG5, ATG7 and JAK2, affecting autophagy, STAT3 phosphorylation and adipogenesis. FTO knockout increases the m 6 A levels of CCND1 and CDK2, blocking MCE. m 6 A, N6-methyladenine; METTL, methyltransferase-like; PTH1R, parathyroid hormone 1 receptor; JAK, Janus kinase; BMSC, bone marrow mesenchymal stem cell; MCE, mitotic clone amplification; FTO, Fat mass and obesity-associated protein; ATG, autophagy-related; STAT3, signal transducer and activator of transcription 3; CCND1, cyclin D1; CDK2, cyclin-dependent kinase 2; IGF2BP1, insulin-like growth factor 2 mRNA-binding protein 1; YTHDF2, YTH domain family 2.

    Journal: International Journal of Molecular Medicine

    Article Title: m 6 A in adipose tissue inflammation: A novel regulator of obesity and metabolic diseases (Review)

    doi: 10.3892/ijmm.2026.5795

    Figure Lengend Snippet: Role of m 6 A in adipogenesis. Insufficient adipogenesis in adipose tissue leads to persistent, chronic inflammation. m 6 A modification plays a crucial role in all stages of adipogenesis, from commitment to terminal differentiation. During commitment, METTL3 promotes lipogenic differentiation in BMSCs by regulating the m 6 A levels of PTH1R and JAK1, whereas silencing METTL14 reduces the expression of SMAD1, inhibiting BMSC proliferation. During terminal differentiation, m 6 A regulates MCE and the transition to mature adipocytes. FTO influences key genes such as ATG5, ATG7 and JAK2, affecting autophagy, STAT3 phosphorylation and adipogenesis. FTO knockout increases the m 6 A levels of CCND1 and CDK2, blocking MCE. m 6 A, N6-methyladenine; METTL, methyltransferase-like; PTH1R, parathyroid hormone 1 receptor; JAK, Janus kinase; BMSC, bone marrow mesenchymal stem cell; MCE, mitotic clone amplification; FTO, Fat mass and obesity-associated protein; ATG, autophagy-related; STAT3, signal transducer and activator of transcription 3; CCND1, cyclin D1; CDK2, cyclin-dependent kinase 2; IGF2BP1, insulin-like growth factor 2 mRNA-binding protein 1; YTHDF2, YTH domain family 2.

    Article Snippet: In addition, for mitotic clone amplification (MCE) in the early stage of terminal differentiation, the inhibition of FTO expression in 3T3-L1 cells leads to increased m 6 A methylation levels of cyclin D1 (CCND1) and cyclin-dependent kinase 2, the protein expression of which is reduced after recognition by YTHDF2, resulting in blockade of the MCE process and in turn the inhibition of lipogenesis ( ) ( ).

    Techniques: Modification, Expressing, Phospho-proteomics, Knock-Out, Blocking Assay, Amplification, Binding Assay