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plex ythdf2  (Addgene inc)


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    Addgene inc plex ythdf2
    Plex Ythdf2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdf2/YTHDF2+(Plasmid+%23156132)/pmc06561033-170-6-19
    Average 94 stars, based on 15 article reviews
    plex ythdf2 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: Transcriptomic analysis of the m6A reader YTHDF2 in the maintenance and differentiation of human embryonic stem cells.
    Article Snippet: gRNA sequences with sticky ends were synthesized (Beijing Tsingke Biotech Co., Ltd.) and inserted into the PX459 plasmid.

    Article Title: Transcriptomic analysis of the m 6 A reader YTHDF2 in the maintenance and differentiation of human embryonic stem cells
    Article Snippet: gRNA sequences with sticky ends were synthesized (Beijing Tsingke Biotech Co., Ltd.) and inserted into the PX459 plasmid.

    Knockdown:

    Article Title: Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis
    Article Snippet: .. To generate YTHDF2 or UBE3C knockdown vectors, specific shRNAs were cloned and inserted into the pLKO.1 backbone (10878; Addgene, Watertown USA). .. KGN cells were transfected using Lipofectamine 2000 (11668019; Invitrogen) and cultured in Opti-MEM for 48 h. The sequences of the shRNAs used are listed in Supplementary Table S3 .

    Article Title: Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis.
    Article Snippet: .. To generate YTHDF2 or UBE3C knockdown vectors, specific shRNAs were cloned and inserted into the pLKO.1 backbone (10878; Addgene, Watertown USA). .. KGN cells were transfected using Lipofectamine 2000 (11668019; Invitrogen) and cultured in Opti-MEM for 48 h. The sequences of the shRNAs used are listed in Supplementary Table S3.

    Clone Assay:

    Article Title: Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis
    Article Snippet: .. To generate YTHDF2 or UBE3C knockdown vectors, specific shRNAs were cloned and inserted into the pLKO.1 backbone (10878; Addgene, Watertown USA). .. KGN cells were transfected using Lipofectamine 2000 (11668019; Invitrogen) and cultured in Opti-MEM for 48 h. The sequences of the shRNAs used are listed in Supplementary Table S3 .

    Article Title: K542 acetylation promotes YTHDF2 binding to m6A-modified mRNAs and fosters colorectal cancer progression.
    Article Snippet: N6-methyladenosine (m6A) modification serves as a crucial regulator of diverse biological processes and disease progression.. A central player in m6A regulation is YT521-B homology domain family member 2 (YTHDF2), a well-characterized m6A-binding protein primarily involved in mRNA stabilization.. This study identifies lysine 542 (K542) as a critical acetylation site on YTHDF2, regulated by p300 as the acetyltransferase and SIRT2 as the deacetylase.

    Article Title: Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis.
    Article Snippet: .. To generate YTHDF2 or UBE3C knockdown vectors, specific shRNAs were cloned and inserted into the pLKO.1 backbone (10878; Addgene, Watertown USA). .. KGN cells were transfected using Lipofectamine 2000 (11668019; Invitrogen) and cultured in Opti-MEM for 48 h. The sequences of the shRNAs used are listed in Supplementary Table S3.

    Article Title: YTHDF proteins and m 6 A-RNA clients undergo autophagic turnover during contact inhibition.
    Article Snippet: .. YTHDF2 was cloned into pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 (Addgene plasmid #87803) 49 by replacing the NLS sequence with YTHDF2 sequence. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 was a gift from Andrea Musacchio. .. Subsequently, EGFP-YTHDF2-P2A-mCherry was cloned into the lentiviral vector pCW57-MCS1-P2AMCS2 (Hygro) (Addgene plasmid #80922) to have pCW57-HygB-EGFP-YTHDF2-P2A-mCherry. pCW57-MCS1-P2A-MCS2 (Hygro) was a gift from Adam Karpf. c-myc-PT3EF1a (Plasmid #92046) c-myc-PT3EF1a was a gift from Xin Chen and was obtained from Addgene. pLARTA_lacZ was used as backbone to clone the pLARTA-Luciferase. pLARTA_lacZ was a gift from Markus Kretz.

    Over Expression:

    Article Title: YTH N 6 -methyladenosine RNA Binding Protein 1 Inhibits Smooth Muscle Cell Phenotypic Modulation and Neointimal Hyperplasia.
    Article Snippet: .. The SNAP-tagged YTHDF1 and YTHDF2 overexpression plasmids were purchased from Addgene (Cat# 155346 and 155347, Watertown, MA, USA) [9]. .. The YTHDF1, YTHDF2, or control (pcDNA) plasmids were electroporated into rat SMCs by using Neon Transfection System (Thermo Fisher Scientific, MPK5000, Waltham, MA, USA) according to the manufacturer’s instruction.

    Article Title: m6A RNA methylation modulates antiviral response in celiac disease.
    Article Snippet: For METTL3 overexpression, a commercially available plasmid from Addgene (#53739) was used. .. For YTHDF2 overexpression a commercially available plasmid from Addgene (#52300) was used. .. For IRF7 overexpression, a CMV driven overexpression vector with coding sequence of IRF7 was purchased from VectorBuilder.

    Plasmid Preparation:

    Article Title: YTHDF proteins and m 6 A-RNA clients undergo autophagic turnover during contact inhibition.
    Article Snippet: .. YTHDF2 was cloned into pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 (Addgene plasmid #87803) 49 by replacing the NLS sequence with YTHDF2 sequence. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 was a gift from Andrea Musacchio. .. Subsequently, EGFP-YTHDF2-P2A-mCherry was cloned into the lentiviral vector pCW57-MCS1-P2AMCS2 (Hygro) (Addgene plasmid #80922) to have pCW57-HygB-EGFP-YTHDF2-P2A-mCherry. pCW57-MCS1-P2A-MCS2 (Hygro) was a gift from Adam Karpf. c-myc-PT3EF1a (Plasmid #92046) c-myc-PT3EF1a was a gift from Xin Chen and was obtained from Addgene. pLARTA_lacZ was used as backbone to clone the pLARTA-Luciferase. pLARTA_lacZ was a gift from Markus Kretz.

    Article Title: m6A RNA methylation modulates antiviral response in celiac disease.
    Article Snippet: For METTL3 overexpression, a commercially available plasmid from Addgene (#53739) was used. .. For YTHDF2 overexpression a commercially available plasmid from Addgene (#52300) was used. .. For IRF7 overexpression, a CMV driven overexpression vector with coding sequence of IRF7 was purchased from VectorBuilder.

    Sequencing:

    Article Title: YTHDF proteins and m 6 A-RNA clients undergo autophagic turnover during contact inhibition.
    Article Snippet: .. YTHDF2 was cloned into pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 (Addgene plasmid #87803) 49 by replacing the NLS sequence with YTHDF2 sequence. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1 was a gift from Andrea Musacchio. .. Subsequently, EGFP-YTHDF2-P2A-mCherry was cloned into the lentiviral vector pCW57-MCS1-P2AMCS2 (Hygro) (Addgene plasmid #80922) to have pCW57-HygB-EGFP-YTHDF2-P2A-mCherry. pCW57-MCS1-P2A-MCS2 (Hygro) was a gift from Adam Karpf. c-myc-PT3EF1a (Plasmid #92046) c-myc-PT3EF1a was a gift from Xin Chen and was obtained from Addgene. pLARTA_lacZ was used as backbone to clone the pLARTA-Luciferase. pLARTA_lacZ was a gift from Markus Kretz.



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    List of antibodies used for this study

    Journal: Experimental Hematology & Oncology

    Article Title: Tumor-intrinsic NSUN2 orchestrates immunosuppression in lung adenocarcinoma via the m 5 C-HDAC8-CCL5 axis

    doi: 10.1186/s40164-026-00826-9

    Figure Lengend Snippet: List of antibodies used for this study

    Article Snippet: A25318 , Anti-YTHDF2 , ABclonal.

    Techniques: Control

    The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: The differentiated expression of YTHDF1 and YTHDF2 in tumor tissues and normal tissues. (A) YTHDF1 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (B) YTHDF1 expression in paired lung cancer tissues and normal tissues in the TCGA database. (C) YTHDF2 expression in non-paired lung cancer tissues and normal tissues according to the TCGA dataset. (D) YTHDF2 expression in paired lung cancer tissues and normal tissues in the TCGA database. ns, not significant; *, P<0.05; ***, P<0.001. TCGA, The Cancer Genome Atlas.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Relationship between YTHDF1/YTHDF2 expression and prognosis of LUSC patients. (A,C) Association between YTHDF1, YTHDF2 expression and DSS of LUSC patients. (B,D) Association between YTHDF1, YTHDF2 expression and OS of LUSC patients. DSS, disease-specific survival; LUSC, lung squamous cell carcinoma; OS, overall survival.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing

    Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Potential molecular mechanisms of YTHDF1 and YTHDF2 in LUSC progression. (A,C) Gene set enrichment analysis showed that YTHDF1 (A) and YTHDF2 (C) high expression subgroups of LUSC patients were significantly associated with E2F targets, G2M checkpoint, mitotic spindle, pancreas beta cells, spermatogenesis and other pathways. (B,D) Gene set enrichment analysis showed that YTHDF1 (B) and YTHDF2 (D) low expression subgroups of LUSC patients were significantly associated with allograft rejection, coagulation, inflammatory response, interferon gamma response and other pathways. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Coagulation

    Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Correlation of YTHDF2 expression levels with LUSC immune cell infiltration. (A) Resting memory CD4+T cells. (B) Memory B cells. (C) Plasma cells. (D) Activated memory CD4+T cells. LUSC, lung squamous cell carcinoma.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Expressing, Clinical Proteomics

    Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 (A) and YTHDF2 (B) expression levels in tumor tissues and adjacent tissues. IOD, integrated optical density.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing

    Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Journal: Journal of Thoracic Disease

    Article Title: Expression of YTHDF1 and YTHDF2 in lung squamous cell carcinoma and their correlation with PD-L1

    doi: 10.21037/jtd-2026-1-0312

    Figure Lengend Snippet: Comparison of YTHDF1 positive (A), YTHDF1 negative (B), YTHDF2 positive (C) and YTHDF2 negative (D) expression.

    Article Snippet: YTHDF2 antibodies were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China), and Ethylene Glycol-bis (2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid (EGTA) antigen retrieval buffer (pH 9.0), antibody diluent, polylysine, phosphate buffered saline (PBS), super-sensitive 3,3'-diaminobenzidine (DAB) substrate, instant immunohistochemistry MaxVision TM kit, and neutral balsam were purchased from Fuzhou Maxim Biotechnology Co., Ltd. (Fuzhou, China).

    Techniques: Comparison, Expressing