ythdf2 (Addgene inc)
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Addgene inc
ythdf2
Ythdf2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf2/pm40495659-103-2-18?v=Addgene+inc
Average 94 stars, based on 15 article reviews
Ythdf2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdf2/pm40495659-103-2-18?v=Addgene+inc
Average 94 stars, based on 15 article reviews
ythdf2 - by Bioz Stars,
2026-08
94/100 stars
Images
1) Product Images from "Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis."
Article Title: Melatonin mitigates ovarian aging through regulation of the YTHDF2/m 6 A/UBE3C axis.
Journal: Acta biochimica et biophysica Sinica
doi: 10.3724/abbs.2025090
Figure Legend Snippet: Figure 1. m6A characteristics after MT treatment in aged mice (A) Representative ovarian histology of 2-month-old mice (young), MT-fed 12- month-old mice (aged + MT), and control 12-month-old mice (Aged). The primary and secondary follicle stages (marked with PF and SF, re spectively) are marked by black arrows. Scale bars, 200 μm (upper) and 20 μm (lower). (B) Quantification of different types of follicles in ovaries from young, aged, and aged + MT mice. Primordial, primary, secondary, corpus luteum, and antral follicles (marked with PmF, PF, SF, CL, and AF, respectively) were counted. n.s., not significant; *P < 0.05. (C) Quantification of the corpus luteum in ovaries from young, aged, and aged + MT mice. (D) Violin plot showing the level of m6A in the ovaries of young, aged and aged + MT mice. n = 5, **** P < 0.0001. (E) Volcano plot showing m6A-modified differentially expressed genes (DEGs) in the ovaries of aged and aged + MT mice (P value < 0.05 and fold change > 2). Down regulated (blue), upregulated (red), and unchanged (gray) genes are indicated. (F) Correlation analysis of 566 m6A DEGs among aged and aged + MT mice. (G) Gene Ontology (GO), KEGG, and Reactome (REAC) pathway analyses of m6A-modified differentially expressed genes. (H) GSEA pathway analysis of the cell cycle. (I,J) Immunofluorescence staining of ovarian GCs from young, aged and aged + MT mice, showing YTHDF2 (red) and DAPI (blue). Scale bar, 50 μm. ***P < 0.001.
Techniques Used: Control, Modification, Immunofluorescence, Staining
Figure Legend Snippet: Figure 2. MT downregulates m6A levels in KGN cells by increasing YTHDF2 expression (A,B) Cell senescence was detected by SA-β-galacto sidase (SA-β-gal) staining in KGN cells under different conditions: without H2O2 (NC), with H2O2 treatment (400 μM, 12 h), and with H2O2 treatment in the presence of MT (300 μM, 12 h). Scale bar, 40 μm. (C, D) KGN cell apoptosis was detected via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Scale bar, 50 μm. (E) m6A dot blot analysis to assess m6A levels in KGN cells under different conditions: without H2O2 (NC), with H2O2 treatment, and with H2O2 treatment in the presence of MT. (F,G) Western blot analysis showing YTHDF2 expression in KGN cells. (H, I) Western blot analysis of YTHDF2 expression in scramble control, YTHDF2 knockdown (shYTHDF2) and MT-treated (300 μM, 12 h) KGN cells in the absence of H2O2 exposure. (J, K) Cell senescence was detected by SA-β-gal staining in scramble control-, shYTHDF2- and MT-treated KGN cells in the absence of H2O2 exposure. Scale bar, 40 μm. (L,M) Cell senescence detected by SA-β-gal staining in NC, H2O2-treated, and YTHDF2- overexpressing KGN cells under H2O2 exposure. Scale bar, 40 μm. Dot blot analysis of m6A levels in scramble control, shYTHDF2- and MT-treated KGN cells in the absence of H2O2 exposure. *P < 0.05, ****P < 0.0001.
Techniques Used: Expressing, Staining, TUNEL Assay, Dot Blot, Western Blot, Control, Knockdown
Figure Legend Snippet: Figure 3. YTHDF2 regulates the m6A level of UBE3C RNA to affect the level of UBE3C expression (A) Correlation analysis of the m6A levels of ubiquitin protein ligases in aged and aged + MT mice. (B) MeRIP‒qPCR analysis showing the relative mRNA expression levels of ubiquitin-related genes in NC, H2O2 and H2O2+MT KGN cells. (C,D) Western blot analysis showing the expressions of YTHDF2, P53, and UBE3C in NC, H2O2 and H2O2 + MT KGN cells. (E,F) Western blot analysis showing the expression of YTHDF2 in scramble-, YTHDF2-knockdown- and MT-treated KGN cells under H2O2-untreated conditions. (G, H) Cell senescence was detected by SA-β-gal staining under H2O2-untreated conditions. Scale bar, 40 μm. (I,J) Cell senescence detected by SA-β-gal staining in NC, H2O2-treated, and UBE3C-overexpressing KGN cells under H2O2 exposure. Scale bar, 40 μm.
Techniques Used: Expressing, Ubiquitin Proteomics, Western Blot, Knockdown, Staining
Figure Legend Snippet: Figure 4. UBE3C upregulates P53 ubiquitination to delay aging (A) Western blot analysis was used to verify the level of P53 ubiquitination in the immunoprecipitates after H2O2 and MT treatment. (B,C) Western blot analysis was used to verify the expressions of P53 and UBE3C after UBE3C was knocked down. (D) qRT-PCR analysis showing the mRNA expression of P53 after UBE3C knockdown. n.s., not significant; ***P < 0.001; ****P < 0.0001. (E,F) Western blot analysis was used to detect the protein expressions of P53 and UBE3C in UBE3C-knockdown and YTHDF2-over expressing KGN cells. (G) Western blot analysis was used to verify the level of P53 ubiquitination in the immunoprecipitates of UBE3C-over expressing and YTHDF2-knockdown KGN cells treated with MG132. (H) Model of the mechanism by which melatonin alleviates ovarian aging in an m6A-dependent manner.
Techniques Used: Ubiquitin Proteomics, Western Blot, Quantitative RT-PCR, Expressing, Knockdown
