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conditional ythdf1 ko allele
Conditional Ythdf1 Ko Allele, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/product/ythdf1/Ythdf1/pm36914671-229-0-8
Average 93 stars, based on 4 article reviews
conditional ythdf1 ko allele - by
Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "Adipocyte YTH N(6)-methyladenosine RNA-binding protein 1 protects against obesity by promoting white adipose tissue beiging in male mice."
Article Title: Adipocyte YTH N(6)-methyladenosine RNA-binding protein 1 protects against obesity by promoting white adipose tissue beiging in male mice.
Journal: Nature communications
doi: 10.1038/s41467-023-37100-z
Figure Legend Snippet: Fig. 1 | Adipose YTHDF1 expression is reduced in obesity. a, b Immunoblot analysis of YTH family proteins in iWAT a and BAT b of mice fed with CD or HFD for 12 weeks. c, d mRNA levels of Yth family genes and thermogenesis-related genes in iWAT c and BAT d of CD- and HFD-fed mice. Data were presented as mean ± SEM
Techniques Used: Expressing, Western Blot
Related Articles
Generated:
Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China). .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China).
CRISPR:
Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China). .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China).
Quantitative RT-PCR:Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: HEK293T ATCC CRL-3216 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025
shRNA:Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: HEK293T ATCC CRL-3216 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025
Recombinant:Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: HEK293T ATCC CRL-3216 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025 .. HCT116 ATCC CCL-247 Ytdhf1 -/- MEF This paper N/A WT MEF This paper N/A Human skin fibroblasts (HSF) Sun et al. 75 N/A YTHDF1 W411A HEK293T This paper N/A Experimental models: Organisms/strains Ythdf1 -/- mice Cyagen company Serial Number: KOCMP-11432-Ythdf1 C57BL/6N Cyagen company N/A Oligonucleotides Gene-specific primers for RT-qPCR in this study This paper see Table S5 shRNA and siRNA sequences used in this study This paper see Table S6 Recombinant DNA FLAG-YTHDF1 This paper N/A FLAG-YTHDF1-Mut This paper N/A FLAG-YTHDF2 This paper N/A FLAG-YTHDF3 This paper N/A (Continued on next page) e3 Molecular Cell 85, 1–17.e1–e8, June 5, 2025
Mutagenesis:Article Title: YTHDF1 differentiates the contributing roles of mTORC1 in aging.
Article Snippet: .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China). .. Generation of homozygous YTHDF1 W411A mutant HEK293T cell line A homozygous YTHDF1 W411A/W411A mutant HEK293T cell line was generated using the CRISPR/Cas9 system by Cyagen Biosciences (Guangzhou, China).
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