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Proteintech ythdc2

Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of <t>YTHDC2</t> , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

Ythdc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdc2/YTHDC2+Antibody/pmc12862939-61-52-53
Average 95 stars, based on 53 article reviews

ythdc2 - by Bioz Stars, 2026-11

95/100 stars

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1) Product Images from "METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition"

Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition

Journal: Frontiers in Veterinary Science

doi: 10.3389/fvets.2025.1732947

Figure Legend Snippet: Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

Techniques Used: Expressing, Methylation, Immunofluorescence, RNA Sequencing

Cell Culture:

Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition
Article Snippet: .. Cultured hepatocytes were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, washed with PBS, and blocked with PBS containing 5% FBS and 0.3% TritonX-100 for 1 h. Thereafter, Primary antibodies against METTL14 (Proteintech, Rosemont, IL, USA) and YTHDC2 (Proteintech) were applied overnight at 4 °C. .. After washing, cells were incubated with the goat anti-rabbit IgG conjugated with Alexa Fluor® 594 (Invitrogen, Waltham, MA, USA) or goat anti-mouse IgG conjugated with Alexa Fluor®488 (Invitrogen) for 1 h, followed by DAPI (Solarbio) nuclear staining.

Western Blot:

Article Title: METTL3-mediated m6A modification of FDX1 confers resistance to cuproptosis and promotes hepatocellular carcinoma progression.
Article Snippet: Supplemented with 10% fetal bovine serum (FBS, ExCell Bio, Shanghai, China). .. The following commercially available antibodies were used for Western blotting: GAPDH (Proteintech, 60004-4-lg), Flag-Tag (Abmart, TT0053), Myc-Tag (Abmart, M20002), METTL3 (Proteintech, 15073-1-AP), METTL14 (Abmart, PC17608), WTAP (Abmart, PA6128), FTO (Abmart, PA2776 ), ALKBH5 (Proteintech, 16837-1-AP), FDX1 (Abmart, ab108257), FMR1 (Proteintech, 13755-1-AP), DLAT (CST, 12362), YTHDF1 (Abmart, P55973R5), YTHDF2 (Abmart, PY100053), YTHDF3 (Abmart, PS05805), YTHDC1(Proteintech, 14392-1-AP), AR TI CL E IN P RE SS YTHDC2(Proteintech, 27779-1-AP), Lipoic acid antibody (Abcam, ab58724), m6A antibody (Synaptic Systems, 2002003). .. Ammonium tetrathiomolybdate (TTM) (Macklin, A828261), Elesclomol (Selleck, STA-4783), STM2457(Glpbio, GC19771), MitoTracker Red CMXRos (Thermo Fisher Scientific, M7512), Z-VAD-FMK (MCE, HY-16658B), 3-Methyladenine (Glpbio, GC10710), Ferrostatin-1 (Glpbio, GC10380).

other:

Article Title: ACTB promotes ESCC progression by regulating the AKT-mTOR signaling pathway through an m 6 A-dependent mechanism.
Article Snippet: Antibodies against ACTB (Cat#20536–1-AP), YTHDC2 (Cat#27779–1-AP), AKT (Cat#10176-2-AP), mTOR (Cat#28273-1-AP), LaminB1 (Cat#12987-1AP), Alpha Tubulin (Cat#11224-1-AP) were purchased from Proteintech (Wuhan, China).

Article Title: m6A RNA methylation modulates antiviral response in celiac disease.
Article Snippet: Following Genes & Immunity electrophoresis, proteins were transferred onto nitrocellulose membranes using a Transblot-Turbo Transfer System (BioRad) and blocked in 5% nonfatty milk diluted in TBST 1X (20mM Tris, 150mM NaCl and 0.1% Tween 20) at room temperature for 1 h. The membranes were incubated overnight at 4 °C with primary antibodies at a 1:1000 dilution.

Incubation:

Article Title: YTHDC1 Orchestrates Telomerase Assembly via Scaffold-Mediated TERT-TERC Interaction.
Article Snippet: .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma- Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C. .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma- Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C.

Article Title: YTHDC1 Orchestrates Telomerase Assembly via Scaffold‐Mediated TERT ‐ TERC Interaction
Article Snippet: .. After washing with PBS, the cells were blocked with 5% BSA for 30 min and incubated with the following antibodies: Flag (1:200; Sigma‐Aldrich, Germany), YTHDF1 (1:200; Proteintech, USA), YTHDF2 (1:200; Proteintech), YTHDF3 (1:200; Santa Cruz Biotechnology, USA), YTHDC1 (1:200; Cell Signaling Technology, USA), YTHDC2 (1:200; Proteintech), or coilin (1:200; Proteintech) overnight at 4°C. ..

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