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ythdc2 interference fragment  (Synbio Technologies LLC)

 
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    Structured Review

    Synbio Technologies LLC ythdc2 interference fragment
    Negative correlation between <t>YTHDC2</t> and RUNX2 in BMSCs osteogenesis. A-B. The mRNA and protein expression of YTHDC2 in BMSCs quantified by qRT-PCR and Western blot. C-D. The mRNA and protein expression of RUNX2 in BMSCs quantified by qRT-PCR and Western blot. (*P < 0.05).
    Ythdc2 Interference Fragment, supplied by Synbio Technologies LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2/ythdc2+interference+fragment/pmc10457424-37-1-8
    Average 90 stars, based on 1 article reviews
    ythdc2 interference fragment - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation"

    Article Title: YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2023.e18876

    Negative correlation between YTHDC2 and RUNX2 in BMSCs osteogenesis. A-B. The mRNA and protein expression of YTHDC2 in BMSCs quantified by qRT-PCR and Western blot. C-D. The mRNA and protein expression of RUNX2 in BMSCs quantified by qRT-PCR and Western blot. (*P < 0.05).
    Figure Legend Snippet: Negative correlation between YTHDC2 and RUNX2 in BMSCs osteogenesis. A-B. The mRNA and protein expression of YTHDC2 in BMSCs quantified by qRT-PCR and Western blot. C-D. The mRNA and protein expression of RUNX2 in BMSCs quantified by qRT-PCR and Western blot. (*P < 0.05).

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of YTHDC2 promoted BMSCs osteogenic differentiation and RUNX2 expression. BMSCs were transfected with YTHDC2-siRNA (YTHDC2-si) or negative control-siRNA (NC), after which they were cultured in OM for 7 days. A-B . The qRT-PCR and Western blot results of YTHDC2 expression in BMSCs. C-D . ALP staining and alizarin red staining of BMSCs. E-F . The qRT-PCR and Western blot results of YTHDC2 expression in BMSCs. (*P < 0.05).
    Figure Legend Snippet: Knockdown of YTHDC2 promoted BMSCs osteogenic differentiation and RUNX2 expression. BMSCs were transfected with YTHDC2-siRNA (YTHDC2-si) or negative control-siRNA (NC), after which they were cultured in OM for 7 days. A-B . The qRT-PCR and Western blot results of YTHDC2 expression in BMSCs. C-D . ALP staining and alizarin red staining of BMSCs. E-F . The qRT-PCR and Western blot results of YTHDC2 expression in BMSCs. (*P < 0.05).

    Techniques Used: Knockdown, Expressing, Transfection, Negative Control, Cell Culture, Quantitative RT-PCR, Western Blot, Staining

    YTHDC2 regulated RUNX2 expression through m6A modification. BMSCs were cultured in osteogenic medium (OM) or growth medium (GM) for 7 days. A. m6A-IP showed OM-induced BMSCs had more m6A modification on RUNX2 mRNA. B. YTHDC2-IP showed YTHDC2 tightly bound to RUNX2 m6A. C. Immunofluorescence staining showed negative correlation between YTHDC2 and RUNX2. D. Fluorescence intensity of OM-induced and GM-induced BMSCs. E. The mechanism of YTHDC2 potential effects on BMSCs osteogenesis through m6A modification on RUNX2 mRNA. (*P < 0.05).
    Figure Legend Snippet: YTHDC2 regulated RUNX2 expression through m6A modification. BMSCs were cultured in osteogenic medium (OM) or growth medium (GM) for 7 days. A. m6A-IP showed OM-induced BMSCs had more m6A modification on RUNX2 mRNA. B. YTHDC2-IP showed YTHDC2 tightly bound to RUNX2 m6A. C. Immunofluorescence staining showed negative correlation between YTHDC2 and RUNX2. D. Fluorescence intensity of OM-induced and GM-induced BMSCs. E. The mechanism of YTHDC2 potential effects on BMSCs osteogenesis through m6A modification on RUNX2 mRNA. (*P < 0.05).

    Techniques Used: Expressing, Modification, Cell Culture, Immunofluorescence, Staining, Fluorescence


    Figure Legend Snippet:

    Techniques Used: Sequencing


    Figure Legend Snippet:

    Techniques Used: Sequencing

    Related Articles

    Negative Control:

    Article Title: YTHDC2 Inhibits Rat Bone Mesenchymal Stem Cells Osteogenic Differentiation by Accelerating RUNX2 mRNA Degradation Via m6A Methylation
    Article Snippet: As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation.. YTHDC2 is an m6A reading protein with the ability to mediate the decay of combined methylated mRNA, however its role in BMSCs osteogenic differentiation remains unknown.. In this study, we first found an increase of RUNX2 expression and a decrease of YTHDC2 expression during the process of BMSCs osteogenic differentiation.

    Article Title: YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation
    Article Snippet: .. The YTHDC2 interference fragment and negative control fragment (Synbio Technologies, China) were co-transfected with Lipofectamine 2000 reagent to downregulate the target gene expression level in BMSCs according to the manufacturer's instructions. .. After transfection in reduced serum medium (Gibco, Australia) for 6 h, the medium was changed into OM and the BMSCs were cultured in OM for further research.

    Targeted Gene Expression:

    Article Title: YTHDC2 Inhibits Rat Bone Mesenchymal Stem Cells Osteogenic Differentiation by Accelerating RUNX2 mRNA Degradation Via m6A Methylation
    Article Snippet: As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation.. YTHDC2 is an m6A reading protein with the ability to mediate the decay of combined methylated mRNA, however its role in BMSCs osteogenic differentiation remains unknown.. In this study, we first found an increase of RUNX2 expression and a decrease of YTHDC2 expression during the process of BMSCs osteogenic differentiation.

    Article Title: YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation
    Article Snippet: .. The YTHDC2 interference fragment and negative control fragment (Synbio Technologies, China) were co-transfected with Lipofectamine 2000 reagent to downregulate the target gene expression level in BMSCs according to the manufacturer's instructions. .. After transfection in reduced serum medium (Gibco, Australia) for 6 h, the medium was changed into OM and the BMSCs were cultured in OM for further research.



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    Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of <t>YTHDC2</t> , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.
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    Image Search Results


    Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Veterinary Science

    Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition

    doi: 10.3389/fvets.2025.1732947

    Figure Lengend Snippet: Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

    Article Snippet: Cultured hepatocytes were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, washed with PBS, and blocked with PBS containing 5% FBS and 0.3% TritonX-100 for 1 h. Thereafter, Primary antibodies against METTL14 (Proteintech, Rosemont, IL, USA) and YTHDC2 (Proteintech) were applied overnight at 4 °C.

    Techniques: Expressing, Methylation, Immunofluorescence, RNA Sequencing