mouse ythdc2 (OriGene)

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OriGene mouse ythdc2

(A) Regimen of tamoxifen treatment in ≥ 8-week-old <t>Ythdc2</t> fl/− Ddx4 -Cre ERT2 male mice. See also . (B) Testis weight (mean ± s.d.) of control ( Ythdc2 fl/− , n = 16) and Ythdc2 iKO males (n = 10 at 2 dpt; 16 at 4 dpt; 3 at 6 dpt, 8 dpt, or 10 dpt). (C) Western blot analysis of cell cycle regulators in control ( Ythdc2 fl/− ), Ythdc2 iKO , and Ythdc2 −/− testes. (D) Histology of control and Ythdc2 iKO (2 dpt) testes. Five stages of seminiferous tubules are shown (VIII-XII). Abbreviations: PreL, preleptotene; Lep, leptotene; Zyg, zygotene; Pa, pachytene; Dip, diplotene; Met, metaphase; RS, round spermatid; ES, elongating spermatid; CS, condensing spermatids. Arrowheads indicate apparently apoptotic pachytene spermatocytes, which are characterized by strong but diffuse eosin stain in the nucleus. Insets show the boxed pachytene spermatocytes with higher magnification in stages VIII-X. Loss of diplotene spermatocytes at the stage XI Ythdc2 iKO tubule is demarcated by a dashed blue line. Scale bar, 50 μm. See also . (E) Surface nuclear spread analysis of control and Ythdc2 iKO (2 dpt) spermatocytes. Absence of TC pachytene cells in control is marked with a large symbol. A severe loss of diplotene cells in Ythdc2 iKO (2 dpt) is indicated by a small symbol. TC, telomere clustering. (F) The plot shows the percentage of each type of spermatocytes in control and Ythdc2 iKO testes (mean ± s.d.) is shown. Eighty-two to 887 cells per mouse and three males per genotype per time point were analyzed. Scale bar, 10 μm. (G) A schematic diagram of spermatocytes in control and Ythdc2 iKO (2 dpt) testes. Lines indicate the presence of spermatocytes. A thinner dash line at 2 dpt (iKO) indicates a severe reduction in the number of diplotene spermatocytes.

Mouse Ythdc2, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdc2/Ythdc2+(NM_001163013)+Mouse+Tagged+ORF+Clone/pmc08720241-326-9-23
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mouse ythdc2 - by Bioz Stars, 2026-09

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1) Product Images from "YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis"

Article Title: YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis

Journal: Cell reports

doi: 10.1016/j.celrep.2021.110110

(A) Regimen of tamoxifen treatment in ≥ 8-week-old Ythdc2 fl/− Ddx4 -Cre ERT2 male mice. See also . (B) Testis weight (mean ± s.d.) of control ( Ythdc2 fl/− , n = 16) and Ythdc2 iKO males (n = 10 at 2 dpt; 16 at 4 dpt; 3 at 6 dpt, 8 dpt, or 10 dpt). (C) Western blot analysis of cell cycle regulators in control ( Ythdc2 fl/− ), Ythdc2 iKO , and Ythdc2 −/− testes. (D) Histology of control and Ythdc2 iKO (2 dpt) testes. Five stages of seminiferous tubules are shown (VIII-XII). Abbreviations: PreL, preleptotene; Lep, leptotene; Zyg, zygotene; Pa, pachytene; Dip, diplotene; Met, metaphase; RS, round spermatid; ES, elongating spermatid; CS, condensing spermatids. Arrowheads indicate apparently apoptotic pachytene spermatocytes, which are characterized by strong but diffuse eosin stain in the nucleus. Insets show the boxed pachytene spermatocytes with higher magnification in stages VIII-X. Loss of diplotene spermatocytes at the stage XI Ythdc2 iKO tubule is demarcated by a dashed blue line. Scale bar, 50 μm. See also . (E) Surface nuclear spread analysis of control and Ythdc2 iKO (2 dpt) spermatocytes. Absence of TC pachytene cells in control is marked with a large symbol. A severe loss of diplotene cells in Ythdc2 iKO (2 dpt) is indicated by a small symbol. TC, telomere clustering. (F) The plot shows the percentage of each type of spermatocytes in control and Ythdc2 iKO testes (mean ± s.d.) is shown. Eighty-two to 887 cells per mouse and three males per genotype per time point were analyzed. Scale bar, 10 μm. (G) A schematic diagram of spermatocytes in control and Ythdc2 iKO (2 dpt) testes. Lines indicate the presence of spermatocytes. A thinner dash line at 2 dpt (iKO) indicates a severe reduction in the number of diplotene spermatocytes.

Figure Legend Snippet: (A) Regimen of tamoxifen treatment in ≥ 8-week-old Ythdc2 fl/− Ddx4 -Cre ERT2 male mice. See also . (B) Testis weight (mean ± s.d.) of control ( Ythdc2 fl/− , n = 16) and Ythdc2 iKO males (n = 10 at 2 dpt; 16 at 4 dpt; 3 at 6 dpt, 8 dpt, or 10 dpt). (C) Western blot analysis of cell cycle regulators in control ( Ythdc2 fl/− ), Ythdc2 iKO , and Ythdc2 −/− testes. (D) Histology of control and Ythdc2 iKO (2 dpt) testes. Five stages of seminiferous tubules are shown (VIII-XII). Abbreviations: PreL, preleptotene; Lep, leptotene; Zyg, zygotene; Pa, pachytene; Dip, diplotene; Met, metaphase; RS, round spermatid; ES, elongating spermatid; CS, condensing spermatids. Arrowheads indicate apparently apoptotic pachytene spermatocytes, which are characterized by strong but diffuse eosin stain in the nucleus. Insets show the boxed pachytene spermatocytes with higher magnification in stages VIII-X. Loss of diplotene spermatocytes at the stage XI Ythdc2 iKO tubule is demarcated by a dashed blue line. Scale bar, 50 μm. See also . (E) Surface nuclear spread analysis of control and Ythdc2 iKO (2 dpt) spermatocytes. Absence of TC pachytene cells in control is marked with a large symbol. A severe loss of diplotene cells in Ythdc2 iKO (2 dpt) is indicated by a small symbol. TC, telomere clustering. (F) The plot shows the percentage of each type of spermatocytes in control and Ythdc2 iKO testes (mean ± s.d.) is shown. Eighty-two to 887 cells per mouse and three males per genotype per time point were analyzed. Scale bar, 10 μm. (G) A schematic diagram of spermatocytes in control and Ythdc2 iKO (2 dpt) testes. Lines indicate the presence of spermatocytes. A thinner dash line at 2 dpt (iKO) indicates a severe reduction in the number of diplotene spermatocytes.

Techniques Used: Control, Western Blot, Staining

(A) TUNEL analysis of paraffin-embedded tissue sections from control and Ythdc2 iKO (2 dpt) testes. The acrosome morphology shown by SP10 immunofluorescence staining was used for seminiferous tubule staging. Nuclear DNA was stained with DAPI. Arrowheads indicate TUNEL-positive pachytene cells. The dashed line in stage IX Ythdc2 iKO demarcates pachytene cells (the inner layer) from leptotene cells (the outer layer). Abbreviations: PreL, preleptotene; Lep, leptotene; Pa, pachytene; RS, round spermatid; ES, elongating spermatid. Scale bar, 50 μm. (B) Percentage of TUNEL-positive tubules from control and Ythdc2 iKO testes at 2 dpt. The mean ± s.d. values were plotted. Two males per genotype (control and Ythdc2 iKO ) were analyzed. Three hundred twenty-three to three hundred ninety-eight tubules were counted for each mouse. (C) Quantification of TUNEL-positive cells in TUNEL-positive tubules (mean ± s.d.) from control and Ythdc2 iKO at 2 dpt. Two males per genotype (control and Ythdc2 iKO ) were analyzed. One hundred seventy-six to two hundred eighty-nine tubules were counted for each mouse.

Figure Legend Snippet: (A) TUNEL analysis of paraffin-embedded tissue sections from control and Ythdc2 iKO (2 dpt) testes. The acrosome morphology shown by SP10 immunofluorescence staining was used for seminiferous tubule staging. Nuclear DNA was stained with DAPI. Arrowheads indicate TUNEL-positive pachytene cells. The dashed line in stage IX Ythdc2 iKO demarcates pachytene cells (the inner layer) from leptotene cells (the outer layer). Abbreviations: PreL, preleptotene; Lep, leptotene; Pa, pachytene; RS, round spermatid; ES, elongating spermatid. Scale bar, 50 μm. (B) Percentage of TUNEL-positive tubules from control and Ythdc2 iKO testes at 2 dpt. The mean ± s.d. values were plotted. Two males per genotype (control and Ythdc2 iKO ) were analyzed. Three hundred twenty-three to three hundred ninety-eight tubules were counted for each mouse. (C) Quantification of TUNEL-positive cells in TUNEL-positive tubules (mean ± s.d.) from control and Ythdc2 iKO at 2 dpt. Two males per genotype (control and Ythdc2 iKO ) were analyzed. One hundred seventy-six to two hundred eighty-nine tubules were counted for each mouse.

Techniques Used: TUNEL Assay, Control, Immunofluorescence, Staining

(A) Volcano plot of expression changes in Ythdc2 iKO versus control ( Ythdc2 fl/− ; HT) pachytene spermatocytes. Only coding genes (13,754) with an average expression of ≥ 50 normalized counts across all the samples are plotted. (B) MA plot of expression changes. The differentially expressed (up and downregulated) genes are shown as red dots. See also and . (C) Expression of differentially expressed genes in various wild-type spermatogenic populations. SSC, spermatogonial stem cells; SC, spermatocytes; RS, round spermatids. ANOVA was performed (p value < 2 × 10 −16 for both upregulated and downregulated genes) and followed by TukeyHSD (p value < 0.001). (D) Expression comparison of genes in Ythdc2 iKO versus control ( Ythdc2 fl/− ; HT) pachytene cells that are enriched or depleted of m 6 A in juvenile testes . (E) Overlap of YTHDC2 RIP-seq targets with the differentially expressed genes in Ythdc2 iKO pachytene cells. (F) Overlap of YTHDC2 RIP-seq targets with the differentially expressed genes in Ythdc2 iKO pachytene cells.

Figure Legend Snippet: (A) Volcano plot of expression changes in Ythdc2 iKO versus control ( Ythdc2 fl/− ; HT) pachytene spermatocytes. Only coding genes (13,754) with an average expression of ≥ 50 normalized counts across all the samples are plotted. (B) MA plot of expression changes. The differentially expressed (up and downregulated) genes are shown as red dots. See also and . (C) Expression of differentially expressed genes in various wild-type spermatogenic populations. SSC, spermatogonial stem cells; SC, spermatocytes; RS, round spermatids. ANOVA was performed (p value < 2 × 10 −16 for both upregulated and downregulated genes) and followed by TukeyHSD (p value < 0.001). (D) Expression comparison of genes in Ythdc2 iKO versus control ( Ythdc2 fl/− ; HT) pachytene cells that are enriched or depleted of m 6 A in juvenile testes . (E) Overlap of YTHDC2 RIP-seq targets with the differentially expressed genes in Ythdc2 iKO pachytene cells. (F) Overlap of YTHDC2 RIP-seq targets with the differentially expressed genes in Ythdc2 iKO pachytene cells.

Techniques Used: Expressing, Control, Comparison

(A) Immunofluorescence of α-tubulin, SUN1, SYCP2 in intact control ( Ythdc2 fl/− ) and Ythdc2 iKO (2 dpt) pachytene spermatocytes. Scale bar, 10μm. See also . (B) Nuclear spread analysis of pachytene, diplotene, and diakinesis/metaphase I (dia/met) spermatocytes after treatment with DMSO or nocodazole. TC, telomere clustering. Scale bar, 10μm. (C) Percentage of spermatocytes (mean ± s.d.) in control ( Ythdc2 fl/− ) and Ythdc2 iKO (2 dpt) after treatment with DMSO or nocodazole. Only pachytene, diplotene, and diakinesis/metaphase I spermatocytes are included. Five hundred thirty-two to nine hundred eighty-five cells were counted per genotype per treatment group. Two males per genotype per experiment were used. The experiments were performed three times (n = 3). n.s., non-significant. See also .

Figure Legend Snippet: (A) Immunofluorescence of α-tubulin, SUN1, SYCP2 in intact control ( Ythdc2 fl/− ) and Ythdc2 iKO (2 dpt) pachytene spermatocytes. Scale bar, 10μm. See also . (B) Nuclear spread analysis of pachytene, diplotene, and diakinesis/metaphase I (dia/met) spermatocytes after treatment with DMSO or nocodazole. TC, telomere clustering. Scale bar, 10μm. (C) Percentage of spermatocytes (mean ± s.d.) in control ( Ythdc2 fl/− ) and Ythdc2 iKO (2 dpt) after treatment with DMSO or nocodazole. Only pachytene, diplotene, and diakinesis/metaphase I spermatocytes are included. Five hundred thirty-two to nine hundred eighty-five cells were counted per genotype per treatment group. Two males per genotype per experiment were used. The experiments were performed three times (n = 3). n.s., non-significant. See also .

Techniques Used: Immunofluorescence, Control

(A) Requirement of YTHDC2 at the leptotene and pachytene stages. The red cross designates the stage of meiotic arrest in the Ythdc2 or Meioc mutants. Ythdc2 or Meioc global knockout mutants display the same early meiotic arrest, which is also present in the Ythdc2 iKO ( Ythdc2 fl/− Ddx4 -Cre ERT2 ) mutant. In contrast, Ythdc2 iKO pachytene cells undergo apoptosis at the late pachytene stage. The seminiferous tubule stage is shown in Roman numerals. See also . (B) Illustration of telomere distribution in wild-type and Ythdc2 iKO pachytene spermatocytes. Two pairs of homologous chromosomes are depicted. The SUN/KASH proteins that connect telomeres with microtubules are not shown. In contrast with the random distribution of telomeres in wild-type, telomeres in the Ythdc2 iKO pachytene cell are clustered to one pole, where microtubules converge and the cytoplasm is expanded.

Figure Legend Snippet: (A) Requirement of YTHDC2 at the leptotene and pachytene stages. The red cross designates the stage of meiotic arrest in the Ythdc2 or Meioc mutants. Ythdc2 or Meioc global knockout mutants display the same early meiotic arrest, which is also present in the Ythdc2 iKO ( Ythdc2 fl/− Ddx4 -Cre ERT2 ) mutant. In contrast, Ythdc2 iKO pachytene cells undergo apoptosis at the late pachytene stage. The seminiferous tubule stage is shown in Roman numerals. See also . (B) Illustration of telomere distribution in wild-type and Ythdc2 iKO pachytene spermatocytes. Two pairs of homologous chromosomes are depicted. The SUN/KASH proteins that connect telomeres with microtubules are not shown. In contrast with the random distribution of telomeres in wild-type, telomeres in the Ythdc2 iKO pachytene cell are clustered to one pole, where microtubules converge and the cytoplasm is expanded.

Techniques Used: Knock-Out, Mutagenesis

Figure Legend Snippet:

Techniques Used: Recombinant, Software

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mouse ythdc2 (OriGene)

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1) Product Images from "YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis"

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