ubiquitinated ythdc2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc ubiquitinated ythdc2
Ubiquitinated Ythdc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdc2/YTHDC2+Antibody/pm41616882-171-5-15
Average 94 stars, based on 14 article reviews

ubiquitinated ythdc2 - by Bioz Stars, 2026-11

94/100 stars

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other:

Article Title: Inhibition of BRD proteins suppresses the phenotype of uterine fibroids
Article Snippet: Specific protein bands were visualized using ChemiDoc XRS molecular imager (Bio-Rad, Hercules, CA, USA).

Incubation:

Article Title: N6-methyladenosine RNA landscape in the aged mouse hearts
Article Snippet: .. Primary antibody incubation occurred at 4 °C overnight and secondary incubation occurred at room temperature for 1 h. Primary antibodies used were: anti p16 (1:500, Santa), anti p53 (1:1,000, CST), anti γH2A.X (1:1,000, Santa), anti METTL3 (1:1,000, Abcam), anti METTL14 (1:1,000, CST), anti WTAP (1:1,000, Proteintech), anti FTO (1:1,000, CST), anti ALKBH5 (1:1,000, Proteintech), anti YTHDC1 (1:1,000, CST), anti YTHDC2 (1:1,000, CST), anti YTHDF1 (1:1,000, CST), anti YTHDF2 (1:1,000, CST), anti YTHDF3 (1:1,000, CST) and anti GAPDH (1:10,000, Proteintech). .. Finally, SuperSignalTM ECL substrate (Thermo Scientific) was incubated with membranes for 2 min at room temperature and then exposed using the BioRad Chemidoc® imager.

Immunoprecipitation:

Article Title: Fuzheng Shengbai Decoction Enhances Antitumor Immunity via YTHDC2-Dependent Stabilization of CLCA2 mRNA in Colorectal Cancer.
Article Snippet: Ethnopharmacological relevance: Traditional Chinese medicine (TCM) has demonstrated multiple therapeutic advantages in colorectal cancer (CRC) management.. The herbal formula Fuzheng Shengbai Decoction (FZSB) has been widely used in clinical practice with well-documented therapeutic efficacy; nevertheless, the mechanisms underlying its anti-CRC effects remain largely unexplored.. Aim of the study: To evaluate the therapeutic efficacy of FZSB in colorectal cancer and its potential underlying mechanisms.

Western Blot:

Control:

Wilms Tumor Assay:

Article Title: Identification of differential <scp>m6A RNA</scp> methylomes and <scp>ALKBH5</scp> as a potential prevention target in the developmental neurotoxicity induced by multiple sevoflurane exposures
Article Snippet: © 2024 Federation of American Societies for Experimental Biology.. Xiaowen Meng, Yichan Wang and Weiming Zhao contributed equally to this work.. Abbreviations: 3′UTRs, 3′ untranslated regions; 5′UTRs, 5′ untranslated regions; ALKBH5, alkB homolog 5; AMPK, adenosine 5′monophosphate (AMP)activated protein kinase; Aqp4, aquaporin4; Atg4d, autophagyrelated 4D cysteine peptidase; B3galt2, β1,3Galactosyltransferase 2; BCA, bicinchoninic acid; Bod1l, biorientation of chromosomes in cell division protein 1like; CBLL1, Casitas Blineage protooncogene like 1; CDSs, coding sequences; Chst1, carbohydrate sulfotransferase 1; DEGs, differentially expressed genes; DMGs, differentially methylated m6A genes; Dnajb4, DnaJ heatshockprotein family (Hsp40) member B4; Dnmbp, dynaminbinding protein; ECL, electrochemiluminescence; ELAVL1, embryonic lethalabnormal vision like protein 1; EPM, elevated plus maze; FMR1, fragile X mentalretardation 1; FTO, fat mass and obesityassociated protein; Gigyf1, GRB10 interacting GYF protein 1; GO, gene ontology; Gpr22, Gprotein coupled receptor 22; HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; HNRNPC, heterogeneous nuclear ribonucleoprotein C; HRP, horseradish peroxidase; Hspa5, heatshock protein family A member 5; IGF2BP1, insulinlike growth factor2 mRNAbinding protein 1; IP, immunoprecipitation; Jph1, junctophilin 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LRPPRC, leucinerich pentatricopeptide repeat containing; m6A, N6Methyladenosine; m6Aseq, m6Asequencing; Manf, mesencephalic astrocytederived neurotrophic factor; MBP, myelin basic protein; METTL14, methyltransferaselike 14; METTL3, methyltransferaselike 3; mRNAs, messenger RNAs; MWM, Morris water maze; NOR, novel object recognition; O.C.T., optimal cutting temperature; Pcdhgb8, protocadherin gamma subfamily B8; Phf3, PHD finger protein 3; PND, postnatal days; PPI, protein–protein interaction; PVDF, polyvinylidene fluoride; Rbm12b2, RNAbinding protein 12BB; RBM15, RNAbinding motif protein 15; RBM15B, RNAbinding motif protein 15B; RIN, RNA integrity number; RNAseq, RNAsequencing; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; Slc25a37, solute carrier family 25 member 37; Spef1, sperm flagellar protein 1; Traf6, tumor necrosis factor receptorassociated factor 6; TSS, transcription starting site; WTAP, Wilms tumor 1associated protein; YTHDC, YTH domain containing; YTHDF, YTH domain family; ZC3H13, zinc finger CCCHtype containing 13.

Magnetic Beads:

Article Title: FTO deficiency in older livers exacerbates ferroptosis during ischaemia/reperfusion injury by upregulating ACSL4 and TFRC
Article Snippet: To demonstrate the binding of m6A reader proteins to Tfrc/Acsl4 mRNA, THLE-2 cells were transfected with indicated plasmids for 48 h. Then the cells were harvest on ice using IP lysis buffer (P0013, Beyotime, Shanghai, China) supplemented with RNase Inhibitor and Protease Inhibitor Cocktail (HY-K1033, MCE, NJ, USA). .. Add YTHDF2 or YTHDC2 antibody (CST, Danvers, MA, USA) to pre-washed Protein A/G magnetic beads (Thermo Fisher, USA) and incubate with rotation for 2 h at room temperature. ..

Review