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rabbit anti human ythdc2 polyclonal antibody  (Bethyl)


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    Bethyl rabbit anti human ythdc2 polyclonal antibody
    Fig. 4. Posttranscriptional regulation of CES2 by RNA methylation via m6A reader protein <t>YTHDC2.</t> The stability of CES2 mRNA in siRNA-transfected HepG2 cells was examined (A-C). HepG2 cells were treated with 10 ng/µL α-amanitin 48 h after transfection with siMETTL3 and siMETTL14 (A), siFTO (B), siALKBH5 (C), or siControl (A-C). Total RNA was prepared after 0, 12, 24, and 36 h. The CES2 mRNA level was determined by using real-time RT-PCR. The CES2 mRNA levels at time 0 (the time of addition of α-amanitin) in each treatment were assigned values of 100%. YTHDC2 mRNA, YTHDF2 mRNA (D), CES2 mRNA (E), and CES2 protein (F) levels in siYTHDC2- or siYTHDF2-transfected HepG2 cells were determined by real-time RT-PCR and Western blotting. The mRNA and protein levels were normalized to β-actin levels. The values represent the levels relative to siControl. (G) Cell lysates from HepG2 cells were immunoprecipitated with an anti-human YTHDC2 antibody or normal rabbit IgG. An electropherogram of the PCR amplicon using primers for CES2 mRNA is shown. The length of the PCR product was 316 bp. Each point and column represent the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.01 compared with siControl.
    Rabbit Anti Human Ythdc2 Polyclonal Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2/pm34536357-51-1-8?v=Bethyl
    Average 94 stars, based on 70 article reviews
    rabbit anti human ythdc2 polyclonal antibody - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "m 6 A modification impacts hepatic drug and lipid metabolism properties by regulating carboxylesterase 2."

    Article Title: m 6 A modification impacts hepatic drug and lipid metabolism properties by regulating carboxylesterase 2.

    Journal: Biochemical pharmacology

    doi: 10.1016/j.bcp.2021.114766

    Fig. 4. Posttranscriptional regulation of CES2 by RNA methylation via m6A reader protein YTHDC2. The stability of CES2 mRNA in siRNA-transfected HepG2 cells was examined (A-C). HepG2 cells were treated with 10 ng/µL α-amanitin 48 h after transfection with siMETTL3 and siMETTL14 (A), siFTO (B), siALKBH5 (C), or siControl (A-C). Total RNA was prepared after 0, 12, 24, and 36 h. The CES2 mRNA level was determined by using real-time RT-PCR. The CES2 mRNA levels at time 0 (the time of addition of α-amanitin) in each treatment were assigned values of 100%. YTHDC2 mRNA, YTHDF2 mRNA (D), CES2 mRNA (E), and CES2 protein (F) levels in siYTHDC2- or siYTHDF2-transfected HepG2 cells were determined by real-time RT-PCR and Western blotting. The mRNA and protein levels were normalized to β-actin levels. The values represent the levels relative to siControl. (G) Cell lysates from HepG2 cells were immunoprecipitated with an anti-human YTHDC2 antibody or normal rabbit IgG. An electropherogram of the PCR amplicon using primers for CES2 mRNA is shown. The length of the PCR product was 316 bp. Each point and column represent the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.01 compared with siControl.
    Figure Legend Snippet: Fig. 4. Posttranscriptional regulation of CES2 by RNA methylation via m6A reader protein YTHDC2. The stability of CES2 mRNA in siRNA-transfected HepG2 cells was examined (A-C). HepG2 cells were treated with 10 ng/µL α-amanitin 48 h after transfection with siMETTL3 and siMETTL14 (A), siFTO (B), siALKBH5 (C), or siControl (A-C). Total RNA was prepared after 0, 12, 24, and 36 h. The CES2 mRNA level was determined by using real-time RT-PCR. The CES2 mRNA levels at time 0 (the time of addition of α-amanitin) in each treatment were assigned values of 100%. YTHDC2 mRNA, YTHDF2 mRNA (D), CES2 mRNA (E), and CES2 protein (F) levels in siYTHDC2- or siYTHDF2-transfected HepG2 cells were determined by real-time RT-PCR and Western blotting. The mRNA and protein levels were normalized to β-actin levels. The values represent the levels relative to siControl. (G) Cell lysates from HepG2 cells were immunoprecipitated with an anti-human YTHDC2 antibody or normal rabbit IgG. An electropherogram of the PCR amplicon using primers for CES2 mRNA is shown. The length of the PCR product was 316 bp. Each point and column represent the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.01 compared with siControl.

    Techniques Used: Methylation, Transfection, Quantitative RT-PCR, Western Blot, Immunoprecipitation, Amplification



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    Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of <t>YTHDC2</t> , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.
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    Image Search Results


    Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Veterinary Science

    Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition

    doi: 10.3389/fvets.2025.1732947

    Figure Lengend Snippet: Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

    Article Snippet: Cultured hepatocytes were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, washed with PBS, and blocked with PBS containing 5% FBS and 0.3% TritonX-100 for 1 h. Thereafter, Primary antibodies against METTL14 (Proteintech, Rosemont, IL, USA) and YTHDC2 (Proteintech) were applied overnight at 4 °C.

    Techniques: Expressing, Methylation, Immunofluorescence, RNA Sequencing