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ythdc2 human cdna  (OriGene)


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    Structured Review

    OriGene ythdc2 human cdna
    Ythdc2 Human Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2+human+cdna/PCMV6-Neo+Mammalian+Expression+Vector/ppr0403045-65-20-32
    Average 96 stars, based on 456 article reviews
    ythdc2 human cdna - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Regulation of translation of expressed genes
    Article Snippet: .. Experimental Vector Construction The human IL-4 cDNA sequence (genbank: NM000589) was removed from plasmid pCMV6-XL5mod_IL4_NM_000589 (OriGene Technologies, Rockville MD) after restriction with SacI and HindIII, and ligated into mammalian expression vector pCMV6-neo (OriGene Technologies) after restriction with the same enzymes. ..

    Plasmid Preparation:

    Article Title: Regulation of translation of expressed genes
    Article Snippet: .. Experimental Vector Construction The human IL-4 cDNA sequence (genbank: NM000589) was removed from plasmid pCMV6-XL5mod_IL4_NM_000589 (OriGene Technologies, Rockville MD) after restriction with SacI and HindIII, and ligated into mammalian expression vector pCMV6-neo (OriGene Technologies) after restriction with the same enzymes. ..

    Article Title: Unraveling small non-coding RNAs with a significant post-transcriptional impact on breast cancer cell signaling, using a combinational sequencing approach
    Article Snippet: .. From the aforementioned workflow, miR-22 emerged as a promising molecule to further elucidate. mir-22 as well as upstream and downstream regulatory regions were cloned into the pCMV6-Neo vector (OriGene Technologies Inc., Rockville, MD, USA). .. For this purpose, a PCR reaction for the amplification of the selected region was performed using specific primers (Table ), DNA from the MDA-MB-468 cell line, and KAPA Hi-Fi Hotstart Ready mix (Kapa Biosystems Inc.).

    Article Title: Functional and clinical significance of novel SERPINA1 variants on alpha-1 antitrypsin deficiency.
    Article Snippet: .. Site-directed mutagenesis Expression vector encoding M1(Val213) AAT based on plasmid pCMV6 (OriGene) was used for in vitro expression of the newly identified variants. .. The Z variant and the new variants were introduced by site-directed mutagenesis using the QuikChange II SiteDirected Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions and the primers described in Table S1.

    Expressing:

    Article Title: Regulation of translation of expressed genes
    Article Snippet: .. Experimental Vector Construction The human IL-4 cDNA sequence (genbank: NM000589) was removed from plasmid pCMV6-XL5mod_IL4_NM_000589 (OriGene Technologies, Rockville MD) after restriction with SacI and HindIII, and ligated into mammalian expression vector pCMV6-neo (OriGene Technologies) after restriction with the same enzymes. ..

    Article Title: Expression of MIR155HG, LOC283856, KIAA0125, and LOC100190986 as potential prognostic and predictive biomarkers for breast cancer
    Article Snippet: MCF-7 cell line was purchased from the ATCC (American Type Culture Collection, USA) and maintained in RPMI medium supplemented with 10% fetal bovine serum (GIBCO, USA) containing antibiotic-antimycotic (100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL of fungizone) (GIBCO) in an incubator at 37°C under an atmosphere of 5% CO 2 . .. MCF-7 cells were stably transfected with the empty expression vector pCMV6-Neo (empty vector) or containing the complete cDNA of the SPARC gene (pCMV6-SPARC) (Origene, USA). .. For the transfection assay, 1×10 5 MCF7 cells were distributed in 6-well plates, and after achieving the necessary confluence (60-70%), they were transfected using the Turbofectin 8.0 reagent (Origene), following the manufacturer's instructions.

    Article Title: Functional and clinical significance of novel SERPINA1 variants on alpha-1 antitrypsin deficiency.
    Article Snippet: .. Site-directed mutagenesis Expression vector encoding M1(Val213) AAT based on plasmid pCMV6 (OriGene) was used for in vitro expression of the newly identified variants. .. The Z variant and the new variants were introduced by site-directed mutagenesis using the QuikChange II SiteDirected Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions and the primers described in Table S1.

    Clone Assay:

    Article Title: Unraveling small non-coding RNAs with a significant post-transcriptional impact on breast cancer cell signaling, using a combinational sequencing approach
    Article Snippet: .. From the aforementioned workflow, miR-22 emerged as a promising molecule to further elucidate. mir-22 as well as upstream and downstream regulatory regions were cloned into the pCMV6-Neo vector (OriGene Technologies Inc., Rockville, MD, USA). .. For this purpose, a PCR reaction for the amplification of the selected region was performed using specific primers (Table ), DNA from the MDA-MB-468 cell line, and KAPA Hi-Fi Hotstart Ready mix (Kapa Biosystems Inc.).

    other:

    Article Title: Impairment of oxidative metabolism compromises Rad51 recruitment and potentiates PARP inhibitor effectiveness in ovarian cancer.
    Article Snippet: Transfected cells were selected 48 hours (h) later using 5 μg/ml puromycin (Thermo Scientific Chemicals).

    Stable Transfection:

    Article Title: Expression of MIR155HG, LOC283856, KIAA0125, and LOC100190986 as potential prognostic and predictive biomarkers for breast cancer
    Article Snippet: MCF-7 cell line was purchased from the ATCC (American Type Culture Collection, USA) and maintained in RPMI medium supplemented with 10% fetal bovine serum (GIBCO, USA) containing antibiotic-antimycotic (100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL of fungizone) (GIBCO) in an incubator at 37°C under an atmosphere of 5% CO 2 . .. MCF-7 cells were stably transfected with the empty expression vector pCMV6-Neo (empty vector) or containing the complete cDNA of the SPARC gene (pCMV6-SPARC) (Origene, USA). .. For the transfection assay, 1×10 5 MCF7 cells were distributed in 6-well plates, and after achieving the necessary confluence (60-70%), they were transfected using the Turbofectin 8.0 reagent (Origene), following the manufacturer's instructions.

    Transfection:

    Article Title: Expression of MIR155HG, LOC283856, KIAA0125, and LOC100190986 as potential prognostic and predictive biomarkers for breast cancer
    Article Snippet: MCF-7 cell line was purchased from the ATCC (American Type Culture Collection, USA) and maintained in RPMI medium supplemented with 10% fetal bovine serum (GIBCO, USA) containing antibiotic-antimycotic (100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL of fungizone) (GIBCO) in an incubator at 37°C under an atmosphere of 5% CO 2 . .. MCF-7 cells were stably transfected with the empty expression vector pCMV6-Neo (empty vector) or containing the complete cDNA of the SPARC gene (pCMV6-SPARC) (Origene, USA). .. For the transfection assay, 1×10 5 MCF7 cells were distributed in 6-well plates, and after achieving the necessary confluence (60-70%), they were transfected using the Turbofectin 8.0 reagent (Origene), following the manufacturer's instructions.

    Article Title: Development of novel anti-CDH1/E-cadherin monoclonal antibodies for versatile applications
    Article Snippet: .. The pCMV6neo-CDH1-myc-DDK (OriGene Technologies, Inc., Rockville, MD, USA) vector was transfected into LN229 using the Neon transfection system (Thermo Fisher Scientific, Inc.). .. The pCMV6neo-CDH1-myc-DDK (OriGene Technologies, Inc., Rockville, MD, USA) vector was transfected into LN229 using the Neon transfection system (Thermo Fisher Scientific, Inc.).

    Mutagenesis:

    Article Title: Functional and clinical significance of novel SERPINA1 variants on alpha-1 antitrypsin deficiency.
    Article Snippet: .. Site-directed mutagenesis Expression vector encoding M1(Val213) AAT based on plasmid pCMV6 (OriGene) was used for in vitro expression of the newly identified variants. .. The Z variant and the new variants were introduced by site-directed mutagenesis using the QuikChange II SiteDirected Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions and the primers described in Table S1.

    In Vitro:

    Article Title: Functional and clinical significance of novel SERPINA1 variants on alpha-1 antitrypsin deficiency.
    Article Snippet: .. Site-directed mutagenesis Expression vector encoding M1(Val213) AAT based on plasmid pCMV6 (OriGene) was used for in vitro expression of the newly identified variants. .. The Z variant and the new variants were introduced by site-directed mutagenesis using the QuikChange II SiteDirected Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions and the primers described in Table S1.



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    96
    OriGene ythdc2 human cdna
    Ythdc2 Human Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ythdc2+human+cdna/PCMV6-Neo+Mammalian+Expression+Vector/ppr0403045-65-20-32
    Average 96 stars, based on 1 article reviews
    ythdc2 human cdna - by Bioz Stars, 2026-11
    96/100 stars
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