xenium cell segmentation add-on kit (2 rxns) (10X Genomics)

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10X Genomics xenium cell segmentation add-on kit (2 rxns)
Xenium Cell Segmentation Add On Kit (2 Rxns), supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xenium+cell+segmentation+kit/macs+dead+cell+removal+kit/pmc12151738-20-0-8
Average 90 stars, based on 1 article reviews

xenium cell segmentation add-on kit (2 rxns) - by Bioz Stars, 2026-11

90/100 stars

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Single Cell:

Article Title: Establishment of Drosophila Intestinal Cell Lines
Article Snippet: Appropriate number of cells (L10: 16503 cells, L15: 17139 cells, L18: 19564 cells) were loaded on a multiple-channel micro-fluidics chip of the Chromium Single Cell Instrument (10x Genomics). .. Single cell gel beads in emulsion containing barcoded oligonucleotides and reverse transcriptase reagents were generated with the Next Gen single cell reagent kit (10X Genomics). .. Appropriate number of cells (L10: 16503 cells, L15: 17139 cells, L18: 19564 cells) were loaded on a multiple-channel micro-fluidics chip of the Chromium Single Cell Instrument (10x Genomics).

Article Title: Single-cell integration reveals metaplasia in inflammatory gut diseases.
Article Snippet: .. Sorted cells were then loaded onto the Chromium Controller (10x Genomics) using the Single Cell Immune Profiling kits and subsequently sequenced as per the manufacturer’s protocol. ..

Article Title: A protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood.
Article Snippet: Duan et al., STAR Protocols 5, Single-cell analysis of human peripheral blood cells provides insights into innate and adaptive immune systems.. However, robust protocols are essential to ensuring single-cell sequencing data quality and cell viability.. Here, we present a protocol for acquiring high-quality single-cell multi-omics data from human peripheral blood mononuclear cells (PBMCs).

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Article Title: Enhanced Spatial Transcriptomics Analysis of Mouse Lung Tissues Reveals Cell-Specific Gene Expression Changes Associated with Pulmonary Hypertension
Article Snippet: Xenium Cell Segmentation Add-on Kit (2 rxns) , 10X Genomics , PN-1000662.

Article Title: Phenotypic plasticity underlies local invasion and distant metastasis in colon cancer
Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens) HCT116 (adult colorectal carcinoma) ECACC Cat# 91091005, RRID:CVCL_0291 For sorting of subpopulations; see Materials and methods, section Flow cytometry and sorting Cell line (Homo sapiens) HCT116- WT, -P, and -MT (wild type, hetero- and homozygous for the b- catenin Ser45del mutant allele) Author:A.K. (Kim et al., 2019) Cell line (Homo sapiens) SW480 (adult colorectal carcinoma) ECACC Cat# 87092801, RRID:CVCL_0546 For sorting of subpopulations, see Materials and methods, section Flow cytometry and sorting Biological sample (Mus musculus) APC- Kras- P53 (AKP) organoids (Apcfl/ fl::KrasG12D/+::Trp53fl/R172H) Author: J.vR (Fumagalli et al., 2017; Fumagalli et al., 2018) Biological sample(Mus musculus) AKP- Zeb1 (AKP- Z) (dox- inducible Zeb1 expression) This study See Materials and methods, section Construction of Zeb1 inducible vector Antibody Anti- mouse CD44- APC (rat monoclonal) BD Pharmingen Cat# 559250, RRID:AB_398661 FACS (1 μg/106 cells) Antibody Anti- human EpCAM- FITC (mouse monoclonal) GeneTex Cat# GTX30708, RRID:AB_1240769 FACS (1 μg/106 cells) Antibody Anti- human EpCAM (mouse monoclonal) Santa Cruz Biotechnology Cat# sc- 66020, RRID:AB_2098654 IF (1:250) Antibody Anti- human ZEB1 (rabbit polyclonal) Santa Cruz Biotechnology Cat# sc- 25388, RRID:AB_2217979 IF (1:200) Antibody Anti- human β-catenin (mouse monoclonal) BD Biosciences Cat# 610154, RRID:AB_397555 IHC (1:500) Antibody Anti- human ZEB1 (rabbit polyclonal) Sigma- Aldrich Cat# HPA027524, RRID:AB_1844977 IHC (1:200) Chemical compound, drug 5- fluorouacil Sigma- Aldrich Cat# F6627 1.5–100 μg/mL Chemical compound, drug Oxaliplatin Sigma- Aldrich Cat# O9512 1.25–20 μg/mL Commercial assay or kit TruSeq Sample Preparation Illumina Cat# 15026495F v.2 Commercial assay or kit Single Cell 3 ́ Reagent Kit Protocol 10XGenomics Cat# CG00052 v2 chemistry Software, algorithm CellRanger 10XGenomics RRID:SCR_017344 Version 2.1.1 Software, algorithm R Seurat, GSVA, MAGIC (Stuart et al., 2019; van Dijk et al., 2018; Hanzelmann et al., 2013) RRID:SCR_007322, RRID:SCR_021058 Version 4.0.4 Software, algorithm Python Velocyto, scVelo (La Manno et al., 2018 ; Bergen et al., 2020) RRID:SCR_018167, RRID:SCR_018168 Version 3.8.3 Sacchetti, Teeuwssen, et al. eLife 2021;10:e61461.

Magnetic Cell Separation:

Article Title: Single-cell RNA sequencing reveals the impact of mechanical loading on knee tibial cartilage in osteoarthritis.
Article Snippet: Articular cartilage degeneration is one of the major pathogenic alterations observed in knee osteoarthritis (KOA).. Mechanical stress has been verified to contribute to KOA development.. To gain insight into the pathogenic mechanism of KOA development, we investigated chondrocyte subsets under different mechanical loading conditions via single-cell RNA sequencing (scRNA-seq).

Article Title: CFTR represses a PDX1 axis to govern pancreatic ductal cell fate
Article Snippet: .. Cells were filtered through a 20 μM strainer and pelleted in 0.04% BSA PBS at 500 g for 10 min. Nonviable dead cells were removed by using MACS Dead Cell Removal Kit following 10X Genomics recommendations (Document CG00039). .. Single cells were counted on a Thermo countess cell counter and 0.04% BSA/PBS was added to achieve a targeted concentration of 1000 cells/μl.

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