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mouse monoclonal wisp2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal wisp2
    Mouse Monoclonal Wisp2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wisp2/WISP-2+Antibody/us12465618-282-21-28
    Average 93 stars, based on 21 article reviews
    mouse monoclonal wisp2 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-Cell Transcriptomics Reveals the Difference of Aortic Atherosclerosis Response to Phytosterols and Oxidation Products of Sterols.
    Article Snippet: Scope: Phytosterols (PS) and sterol oxidation products are key dietary factors influencing atherosclerosis besides cholesterol, although the mechanisms remain elusive.. Recently, single-cell RNA sequencing (scRNA-seq) has revealed the heterogeneity of multiple cell types associated with complex pathogenesis in atherosclerosis development.. Methods and results: Here, scRNA-seq is performed to investigate the alterations in the aortic cells from ApoE−/− mice induced by diet-derived PS or two sterol oxidation products, phytosterols oxidation products (POPs), and cholesterol oxidation products (COPs).

    Article Title: CCN5 negatively regulates TGF-β-induced endometriosis associated fibrosis through Wnt/β-catenin signaling via Smad3-dependent mechanism
    Article Snippet: .. After permeabilization with PBS-T (0.1% Triton X-100 in PBS solution), 5% bovine serum albumin was used to block non-specific interaction for 30 min and then incubated HESCs with primary antibodies against CCN5 (sc-514070, Santa Cruz), β-catenin (#8480, Cell Signaling Technology), Smad3 (#9523, Cell Signaling Technology), α-SMA (#19,245, Cell Signaling Technology), Vimentin (60,330–1-Ig, Proteintech) or CK-18 (66,187–1-Ig, Proteintech) at 4 °C overnight. .. A fluorescence-conjugated secondary antibody solution (1:100; MultiSciences, Hangzhou, China) was incubated for 1 h at room temperature.

    Article Title: Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.
    Article Snippet: .. The lysates were incubated with 1 μg of antibodies against WISP2 (Santa Cruz; sc514070), Flag-tag (Abcam; ab125243) or IgG (Solarbio, SP031) overnight at 4 C with gentle rotation, followed by incubation with 60 μl of Protein A Affinity Chromatography Media (Aladdin; P5362–01) for 2 h. The immunocomplex was washed, denatured, and subjected to Western blotting using primary antibodies against WISP2 (ABclonal, A7456), acetylated-Lysine (ABclonal, A2391), HDAC1 (ABclonal, A19571), HDAC3 (ABclonal, A19537), HDAC6 (ABclonal, A1732), HDAC7 (ABclonal, A13008), Flag-tag (Abcam, ab205606), and NEDD4 (ABclonal, A4385). ..

    Article Title: CCN5 negatively regulates TGF-β-induced endometriosis associated fibrosis through Wnt/β-catenin signaling via Smad3-dependent mechanism.
    Article Snippet: Subsequently, the sections were stained with hematoxylin and eosin (H&E), Masson’s trichrome stain, or Sirius red stain according to the manufacturer’s instructions, and visualized by microscopy (Olympus). .. After permeabilization with PBS-T (0.1% Triton X-100 in PBS solution), 5% bovine serum albumin was used to block non-specific interaction for 30 min and then incubated HESCs with primary antibodies against CCN5 (sc-514070, Santa Cruz), β-catenin (#8480, Cell Signaling Technology), Smad3 (#9523, Cell Signaling Technology), α-SMA (#19,245, Cell Signaling Technology), Vimentin (60,330–1-Ig, Proteintech) or CK-18 (66,187–1-Ig, Proteintech) at 4 °C overnight. .. A fluorescence-conjugated secondary antibody solution (1:100; MultiSciences, Hangzhou, China) was incubated for 1 h at room temperature.

    Blocking Assay:

    Article Title: CCN5 negatively regulates TGF-β-induced endometriosis associated fibrosis through Wnt/β-catenin signaling via Smad3-dependent mechanism
    Article Snippet: .. After permeabilization with PBS-T (0.1% Triton X-100 in PBS solution), 5% bovine serum albumin was used to block non-specific interaction for 30 min and then incubated HESCs with primary antibodies against CCN5 (sc-514070, Santa Cruz), β-catenin (#8480, Cell Signaling Technology), Smad3 (#9523, Cell Signaling Technology), α-SMA (#19,245, Cell Signaling Technology), Vimentin (60,330–1-Ig, Proteintech) or CK-18 (66,187–1-Ig, Proteintech) at 4 °C overnight. .. A fluorescence-conjugated secondary antibody solution (1:100; MultiSciences, Hangzhou, China) was incubated for 1 h at room temperature.

    Article Title: CCN5 negatively regulates TGF-β-induced endometriosis associated fibrosis through Wnt/β-catenin signaling via Smad3-dependent mechanism.
    Article Snippet: Subsequently, the sections were stained with hematoxylin and eosin (H&E), Masson’s trichrome stain, or Sirius red stain according to the manufacturer’s instructions, and visualized by microscopy (Olympus). .. After permeabilization with PBS-T (0.1% Triton X-100 in PBS solution), 5% bovine serum albumin was used to block non-specific interaction for 30 min and then incubated HESCs with primary antibodies against CCN5 (sc-514070, Santa Cruz), β-catenin (#8480, Cell Signaling Technology), Smad3 (#9523, Cell Signaling Technology), α-SMA (#19,245, Cell Signaling Technology), Vimentin (60,330–1-Ig, Proteintech) or CK-18 (66,187–1-Ig, Proteintech) at 4 °C overnight. .. A fluorescence-conjugated secondary antibody solution (1:100; MultiSciences, Hangzhou, China) was incubated for 1 h at room temperature.

    Gentle:

    Article Title: Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.
    Article Snippet: .. The lysates were incubated with 1 μg of antibodies against WISP2 (Santa Cruz; sc514070), Flag-tag (Abcam; ab125243) or IgG (Solarbio, SP031) overnight at 4 C with gentle rotation, followed by incubation with 60 μl of Protein A Affinity Chromatography Media (Aladdin; P5362–01) for 2 h. The immunocomplex was washed, denatured, and subjected to Western blotting using primary antibodies against WISP2 (ABclonal, A7456), acetylated-Lysine (ABclonal, A2391), HDAC1 (ABclonal, A19571), HDAC3 (ABclonal, A19537), HDAC6 (ABclonal, A1732), HDAC7 (ABclonal, A13008), Flag-tag (Abcam, ab205606), and NEDD4 (ABclonal, A4385). ..

    Affinity Chromatography:

    Article Title: Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.
    Article Snippet: .. The lysates were incubated with 1 μg of antibodies against WISP2 (Santa Cruz; sc514070), Flag-tag (Abcam; ab125243) or IgG (Solarbio, SP031) overnight at 4 C with gentle rotation, followed by incubation with 60 μl of Protein A Affinity Chromatography Media (Aladdin; P5362–01) for 2 h. The immunocomplex was washed, denatured, and subjected to Western blotting using primary antibodies against WISP2 (ABclonal, A7456), acetylated-Lysine (ABclonal, A2391), HDAC1 (ABclonal, A19571), HDAC3 (ABclonal, A19537), HDAC6 (ABclonal, A1732), HDAC7 (ABclonal, A13008), Flag-tag (Abcam, ab205606), and NEDD4 (ABclonal, A4385). ..

    Western Blot:

    Article Title: Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.
    Article Snippet: .. The lysates were incubated with 1 μg of antibodies against WISP2 (Santa Cruz; sc514070), Flag-tag (Abcam; ab125243) or IgG (Solarbio, SP031) overnight at 4 C with gentle rotation, followed by incubation with 60 μl of Protein A Affinity Chromatography Media (Aladdin; P5362–01) for 2 h. The immunocomplex was washed, denatured, and subjected to Western blotting using primary antibodies against WISP2 (ABclonal, A7456), acetylated-Lysine (ABclonal, A2391), HDAC1 (ABclonal, A19571), HDAC3 (ABclonal, A19537), HDAC6 (ABclonal, A1732), HDAC7 (ABclonal, A13008), Flag-tag (Abcam, ab205606), and NEDD4 (ABclonal, A4385). ..



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    The expression level and diagnostic efficacy of candidate markers (EFNA1, TYMS and <t>WISP2)</t> in CC and OC. A , C The expression level of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in CC and OC
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    Image Search Results


    qPCR validation of differentially expressed genes. ( A ) GDF5 . ( B ) CCN5 . ( C ) SKIL . For each gene, cumulative results ( n = 5 donors) are showed, together with donors clustered by response to miRNA inhibitor treatment: responders (#162, #217, #255) and non-responders (#152, #157). * p < 0.05

    Journal: Stem Cell Research & Therapy

    Article Title: Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment

    doi: 10.1186/s13287-025-04589-8

    Figure Lengend Snippet: qPCR validation of differentially expressed genes. ( A ) GDF5 . ( B ) CCN5 . ( C ) SKIL . For each gene, cumulative results ( n = 5 donors) are showed, together with donors clustered by response to miRNA inhibitor treatment: responders (#162, #217, #255) and non-responders (#152, #157). * p < 0.05

    Article Snippet: CNN5 , Hs01031984_m1 , , .

    Techniques: Biomarker Discovery

    The expression level and diagnostic efficacy of candidate markers (EFNA1, TYMS and WISP2) in CC and OC. A , C The expression level of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in CC and OC

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: The expression level and diagnostic efficacy of candidate markers (EFNA1, TYMS and WISP2) in CC and OC. A , C The expression level of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in CC and OC

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: Expressing, Diagnostic Assay

    Expression and diagnostic validation of candidate biomarkers (EFNA1, TYMS and WISP2) in CC and OC. A , C Expression comparison of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in the CC and OC

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: Expression and diagnostic validation of candidate biomarkers (EFNA1, TYMS and WISP2) in CC and OC. A , C Expression comparison of 3 candidate biomarkers between tumor and normal groups. B , D The ROC curves of the 3 candidate biomarkers in the CC and OC

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: Expressing, Diagnostic Assay, Biomarker Discovery, Comparison

    Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA). Protein levels of EFNA1、TYMS and WISP2 in A , E , I cervical normal tissue, and B , F , J CC tissue. Protein levels of EFNA1、TYMS and WISP2 in C , G , K ovary normal tissue, and D , H , L OC tissue

    Journal: Discover Oncology

    Article Title: Exploration of the shared pathways and common biomarkers in cervical and ovarian cancer using integrated bioinformatics analysis

    doi: 10.1007/s12672-024-01725-3

    Figure Lengend Snippet: Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA). Protein levels of EFNA1、TYMS and WISP2 in A , E , I cervical normal tissue, and B , F , J CC tissue. Protein levels of EFNA1、TYMS and WISP2 in C , G , K ovary normal tissue, and D , H , L OC tissue

    Article Snippet: B , D The ROC curves of the 3 candidate biomarkers in the CC and OC Fig. 7 Protein levels of EFNA1、TYMS and WISP2 in the normal and the tumor tissues in the Human Protein Atlas database (HPA).

    Techniques: