crispr design webtool (Benchling Inc)
86
Structured Review
Benchling Inc
crispr design webtool
Crispr Design Webtool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/webtool/crispr+design+tool/pmc12900645-220-9-19
Average 86 stars, based on 1 article reviews
Crispr Design Webtool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/webtool/crispr+design+tool/pmc12900645-220-9-19
Average 86 stars, based on 1 article reviews
crispr design webtool - by Bioz Stars,
2026-09
86/100 stars
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Cloning:Article Title: DRP1 and MID49 co-diffusion scans mitochondria for fission. Article Snippet: .. For sgRNA design and cloning, sgRNAs targeting the human DRP1 locus (ENSG00000087470) near the start of the DRP1 open reading frame were designed using the CRISPR:Article Title: DRP1 and MID49 co-diffusion scans mitochondria for fission. Article Snippet: .. For sgRNA design and cloning, sgRNAs targeting the human DRP1 locus (ENSG00000087470) near the start of the DRP1 open reading frame were designed using the Article Title: Deletion of mitochondrial calcium uniporter enhances calcium signals by slowing calcium clearance and triggers adaptive transcriptomic remodeling Article Snippet: .. Guide (g)RNA targeting human MCU (Ensembl Gene ID: ENSG00000156026 ; chromosomal location chr10 72,692,131—72,887,694 [+]; genome: GRCh38 (hg38, Homo sapiens )) was designed using the Article Title: CYLD-mediated lysine63 deubiquitination regulates synaptic transmission and autophagy to mitigate age-related sequelae. Article Snippet: .. A 200bp homology repair (HR) template flanking the mutation site introduced the desired edit TGT→TCT as well as two silent mutations creating a new XbaI site and destroying an existing EcoRI site to facilitate selection of mutagenized lines. crRNA and HR template were designed using the Article Title: Ligustroflavone protects against acute kidney injury by inhibiting ferroptosis via acting on GSK3β/NRF2 signaling Article Snippet: .. GSK3β-knockout (GSK3β-KO) TKPTs constructed: The CRISPR/Cas9 plasmids targeting mouse GSK3β [the guide (g) RNA sequences are presented in ] were constructed by using PX459 (cat. no. 62988; Addgene, Inc.). gRNAs were designed using Benchling's Article Title: Deletion of mitochondrial calcium uniporter enhances calcium signals by slowing calcium clearance and triggers adaptive transcriptomic remodeling Article Snippet: .. Two different guide (g)RNAs targeting rat Mcu (Ensembl Gene ID: ENSRNOG00000045920 ; chromosomal location chr20 29,038,480—29,199,224 [-]; genome: Rnor_6.0 (rn6, Rattus norvegicus )) were designed using the Mutagenesis:Article Title: CYLD-mediated lysine63 deubiquitination regulates synaptic transmission and autophagy to mitigate age-related sequelae. Article Snippet: .. A 200bp homology repair (HR) template flanking the mutation site introduced the desired edit TGT→TCT as well as two silent mutations creating a new XbaI site and destroying an existing EcoRI site to facilitate selection of mutagenized lines. crRNA and HR template were designed using the Selection:Article Title: CYLD-mediated lysine63 deubiquitination regulates synaptic transmission and autophagy to mitigate age-related sequelae. Article Snippet: .. A 200bp homology repair (HR) template flanking the mutation site introduced the desired edit TGT→TCT as well as two silent mutations creating a new XbaI site and destroying an existing EcoRI site to facilitate selection of mutagenized lines. crRNA and HR template were designed using the Injection:Article Title: In vivo base editing alleviates hepatic iron accumulation and fibrosis in models of HFE-related hereditary hemochromatosis. Article Snippet: .. Fig. 4: Off-target analysis and SNP/indel detection in RNA transcripts. (A) C282Y A>G correction rates in different organs of 129-Hfetm1.1Nca/J mice 4 months after injection of 2 mg/kg LNP-ABE-mG17 or PBS (n=4 LNP [n=8 hepatocytes], n=2 PBS [n=9 hepatocytes]), determined by NGS. (B) Nine top candidates of in silico–predicted off-target sites for Next-Generation Sequencing:Article Title: In vivo base editing alleviates hepatic iron accumulation and fibrosis in models of HFE-related hereditary hemochromatosis. Article Snippet: .. Fig. 4: Off-target analysis and SNP/indel detection in RNA transcripts. (A) C282Y A>G correction rates in different organs of 129-Hfetm1.1Nca/J mice 4 months after injection of 2 mg/kg LNP-ABE-mG17 or PBS (n=4 LNP [n=8 hepatocytes], n=2 PBS [n=9 hepatocytes]), determined by NGS. (B) Nine top candidates of in silico–predicted off-target sites for Sequencing:Article Title: In vivo base editing alleviates hepatic iron accumulation and fibrosis in models of HFE-related hereditary hemochromatosis. Article Snippet: .. Fig. 4: Off-target analysis and SNP/indel detection in RNA transcripts. (A) C282Y A>G correction rates in different organs of 129-Hfetm1.1Nca/J mice 4 months after injection of 2 mg/kg LNP-ABE-mG17 or PBS (n=4 LNP [n=8 hepatocytes], n=2 PBS [n=9 hepatocytes]), determined by NGS. (B) Nine top candidates of in silico–predicted off-target sites for Article Title: CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning Article Snippet: .. Arbitrary sequences can be targeted using tools like Benchling for Functional Assay:Article Title: In vivo base editing alleviates hepatic iron accumulation and fibrosis in models of HFE-related hereditary hemochromatosis. Article Snippet: .. Fig. 4: Off-target analysis and SNP/indel detection in RNA transcripts. (A) C282Y A>G correction rates in different organs of 129-Hfetm1.1Nca/J mice 4 months after injection of 2 mg/kg LNP-ABE-mG17 or PBS (n=4 LNP [n=8 hepatocytes], n=2 PBS [n=9 hepatocytes]), determined by NGS. (B) Nine top candidates of in silico–predicted off-target sites for Construct:Article Title: Ligustroflavone protects against acute kidney injury by inhibiting ferroptosis via acting on GSK3β/NRF2 signaling Article Snippet: .. GSK3β-knockout (GSK3β-KO) TKPTs constructed: The CRISPR/Cas9 plasmids targeting mouse GSK3β [the guide (g) RNA sequences are presented in ] were constructed by using PX459 (cat. no. 62988; Addgene, Inc.). gRNAs were designed using Benchling's |
