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Synthego Inc web based tool
Web Based Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/web-based+tool/analysis+crispr+ice+tool/pm41945753-85-9-11
Average 86 stars, based on 1 article reviews
web based tool - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: IMP1 is a novel Post-Transcriptional Regulator of Early Erythroid Development and Embryonic Globin output.
Article Snippet: .. Clone screening was 154 performed by PCR, purifica on, and Sanger sequencing, with inser ons and dele ons (indels) analyzed 155 using Benchling and the Synthego ICE tool. .. 156 157 PCR and RT-qPCR analysis 158 Genomic DNA was extracted and IMP1 fragments amplified using GoTaq polymerase (Thermo 159 Scien fic).

Sequencing:

Article Title: IMP1 is a novel Post-Transcriptional Regulator of Early Erythroid Development and Embryonic Globin output.
Article Snippet: .. Clone screening was 154 performed by PCR, purifica on, and Sanger sequencing, with inser ons and dele ons (indels) analyzed 155 using Benchling and the Synthego ICE tool. .. 156 157 PCR and RT-qPCR analysis 158 Genomic DNA was extracted and IMP1 fragments amplified using GoTaq polymerase (Thermo 159 Scien fic).

other:

Article Title: Protocol for enhancing CRISPR-Cas9 genome editing using histone deacetylase inhibition and engineered virus-like particle delivery
Article Snippet: Synthego Performance Analysis, ICE Analysis. v3.0 , Synthego , https://ice.editco.bio/#/.

Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity.
Article Snippet: Genomic DNA was extracted using the QuickExtract solution (LGC Biosearch Technologies).

Article Title: Expansion and CAR engineering of granulocyte-monocyte progenitors for cellular immunotherapy.
Article Snippet: The 10× Genomics libraries were first sequenced on an Illumina NextSeq 500 aiming at a coverage of 5,000 raw reads per cell to estimate the cell numbers, before being sequenced deeper on an Illumina HiSeq 2500 aiming at a coverage of 50,000 raw reads per cell (paired-end; read1: 26 cycles; i7 index: 8 cycles; read 2: 98 cycles).

Knock-Out:

Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity.
Article Snippet: 18 Cell Reports 45, 117423, June 23, 2026 Knockout of transcription factors using CRISPR/Cas9 Cryopreserved tonsil cells were thawed in a 37◦C water bath into serum-free medium containing 10 μg/mL DNAse (Roche) and incubated for ∼1 h at 37◦C and 5% CO2. .. Knockout was performed using chemically modified synthetic single guide RNAs (gRNAs) formulated in proprietary gene knockout kits (Synthego/EditCo; Table S2) targeting genes encoding the selected transcription factors. ..

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).

Modification:

Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity.
Article Snippet: 18 Cell Reports 45, 117423, June 23, 2026 Knockout of transcription factors using CRISPR/Cas9 Cryopreserved tonsil cells were thawed in a 37◦C water bath into serum-free medium containing 10 μg/mL DNAse (Roche) and incubated for ∼1 h at 37◦C and 5% CO2. .. Knockout was performed using chemically modified synthetic single guide RNAs (gRNAs) formulated in proprietary gene knockout kits (Synthego/EditCo; Table S2) targeting genes encoding the selected transcription factors. ..

Gene Knockout:

Article Title: Aging restricts maturation of CXCL13 + T follicular helper cells in human immunity.
Article Snippet: 18 Cell Reports 45, 117423, June 23, 2026 Knockout of transcription factors using CRISPR/Cas9 Cryopreserved tonsil cells were thawed in a 37◦C water bath into serum-free medium containing 10 μg/mL DNAse (Roche) and incubated for ∼1 h at 37◦C and 5% CO2. .. Knockout was performed using chemically modified synthetic single guide RNAs (gRNAs) formulated in proprietary gene knockout kits (Synthego/EditCo; Table S2) targeting genes encoding the selected transcription factors. ..

Clone Assay:

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).

Generated:

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).

CRISPR:

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).

Single Cell:

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).

Cloning:

Article Title: PHGDH loss promotes hypoxia tolerance through glycolytic reprogramming and enhanced HIF-1 activity.
Article Snippet: Additional media HPLM (A4899101, Gibco) and MEM (21090022, Gibco). .. PHGDH knockout clones were generated using a CRISPR-Cas9 ribonucleoprotein-based genome editing approach in accordance with Synthego’s single-cell cloning workflow (guide sequences Table S1). .. Cells were regularly checked for mycoplasma (Mycoalert mycoplasma detection kit, Lonza).



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