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web‐based ibright image analysis software  (Thermo Fisher)


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    Structured Review

    Thermo Fisher web‐based ibright image analysis software

    Web‐Based Ibright Image Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/web-based+imaging+software/ibright+software/pmc09289529-124-0-6
    Average 90 stars, based on 1 article reviews
    web‐based ibright image analysis software - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Varicella‐Zoster virus ORF9 is an antagonist of the DNA sensor cGAS"

    Article Title: Varicella‐Zoster virus ORF9 is an antagonist of the DNA sensor cGAS

    Journal: The EMBO Journal

    doi: 10.15252/embj.2021109217


    Figure Legend Snippet:

    Techniques Used: Generated, Recombinant, Expressing, Flow Cytometry, Sequencing, Cloning, Labeling, Luciferase, Staining, Protease Inhibitor, Reverse Transcription, Software

    Related Articles

    Software:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: After washing three times with PBS, the protein–antibody immunocomplexes were detected by a chemical reaction with Enhanced Chemiluminescence (ECL) substrate (WBLUC0500, MilliporeSigma) and recorded using an iBright CL1500 imaging system (Thermo Fisher Scientific). .. The images were processed using iBright software (Thermo Fisher Scientific). ..

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. βactin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    Article Title: Developing MiR-133a Zipper Nanoparticles for Targeted Enhancement of Thermogenic Adipocyte Generation.
    Article Snippet: Existing delivery methods for RNAi therapeutics encounter challenges, including stability, specificity, and off-target effects, which restrict their clinical effectiveness.. In this study, a novel miR-133a zipper nanoparticle (NP) system that integrates miRNA zipper technology with rolling circle transcription (RCT) to achieve targeted delivery and specific regulation of miR-133a in adipocytes, is presented.. This innovative approach can greatly enhance the delivery and release of miR-133a zippers, increasing the expression of thermogenic genes and mitochondrial biogenesis. he miR-133a zipper NP is utilized for the delivery of miRNA zipper-blocking miR-133a, an endogenous inhibitor of Prdm16 expression, to enhance the thermogenic activity of adipocytes by modulating their transcriptional program.

    Article Title: High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy.
    Article Snippet: .. The expression level was compared via density analysis using iBright software (Thermo Fisher). .. The effects of two induction variables, i.e., NaCl concentration and treatment time, on the expression of VHSV glycoprotein in transformed Chlorella were evaluated using Design Expert 13 software (Stat-Ease Inc., Minneapolis, MN, USA).

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. β-actin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    Article Title: Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells
    Article Snippet: Densitometry analysis was conducted across 3 independent experiments using ImageJ software. .. Original immunoblot images and additional detailed densitometry method and analysis using the iBright Software (ThermoFisher, Waltham, MA, USA) can be found in the . .. All confocal imaging was conducted on tethered and fixed cells using an Olympus IX81 microscope with a FV-1000 confocal laser scanning system with a 60× oil emersion magnification and objective lens numerical aperture of 1.42.

    Article Title: High OCT4 Expression Might Be Associated with an Aggressive Phenotype in Rectal Cancer.
    Article Snippet: The PVDF membranes were analyzed using an imaging documentation system, using the iBright CL1000 equipment (Thermo ScientificTM Waltham, MA, USA). .. The iBright analysis software desktop version (Thermo ScientificTM Waltham, MA, USA) was used to measure the band densitometry to determine OCT4 expression. .. Statistical data were analyzed using SPSS for Windows, version 21.0 (IBM Corp., Armonk, NY, USA), and GraphPad Prism 8.0.2 (Graphpad Software Inc., San Diego, CA, USA).

    Incubation:

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. βactin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. β-actin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    Imaging:

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma.
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. βactin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    Article Title: Developing MiR-133a Zipper Nanoparticles for Targeted Enhancement of Thermogenic Adipocyte Generation.
    Article Snippet: Existing delivery methods for RNAi therapeutics encounter challenges, including stability, specificity, and off-target effects, which restrict their clinical effectiveness.. In this study, a novel miR-133a zipper nanoparticle (NP) system that integrates miRNA zipper technology with rolling circle transcription (RCT) to achieve targeted delivery and specific regulation of miR-133a in adipocytes, is presented.. This innovative approach can greatly enhance the delivery and release of miR-133a zippers, increasing the expression of thermogenic genes and mitochondrial biogenesis. he miR-133a zipper NP is utilized for the delivery of miRNA zipper-blocking miR-133a, an endogenous inhibitor of Prdm16 expression, to enhance the thermogenic activity of adipocytes by modulating their transcriptional program.

    Article Title: New microRNA-based therapies reveal common targets in paediatric medulloblastoma and adult glioblastoma
    Article Snippet: Following this, the PVDF membranes were incubated with TBST and the appropriate primary antibody antisera against CORO1C (dilution 1:500), SV2B (dilution 1:500), and β-actin (dilution 1:1000) overnight at 4 °C (ProteinTech, Manchester, UK). .. Next, membranes were washed three times with TBST and incubated in the dark with an appropriate HRP-conjugated secondary antibody for 1 h. Band quantification was performed with the iBright Imaging Systems iBright Analysis Software (Applied Biosystems ThermoFisher, Pleasanton, CA) via chemiluminescent detection and visualisation of proteins. β-actin was used as a housekeeping protein. .. All antibodies were purchased by ProteinTech, Manchester, UK.

    other:

    Article Title: Varicella‐Zoster virus ORF9 is an antagonist of the DNA sensor cGAS
    Article Snippet: Web‐based iBright Image Analysis software , Thermo Fisher , .

    Expressing:

    Article Title: High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy.
    Article Snippet: .. The expression level was compared via density analysis using iBright software (Thermo Fisher). .. The effects of two induction variables, i.e., NaCl concentration and treatment time, on the expression of VHSV glycoprotein in transformed Chlorella were evaluated using Design Expert 13 software (Stat-Ease Inc., Minneapolis, MN, USA).

    Article Title: High OCT4 Expression Might Be Associated with an Aggressive Phenotype in Rectal Cancer.
    Article Snippet: The PVDF membranes were analyzed using an imaging documentation system, using the iBright CL1000 equipment (Thermo ScientificTM Waltham, MA, USA). .. The iBright analysis software desktop version (Thermo ScientificTM Waltham, MA, USA) was used to measure the band densitometry to determine OCT4 expression. .. Statistical data were analyzed using SPSS for Windows, version 21.0 (IBM Corp., Armonk, NY, USA), and GraphPad Prism 8.0.2 (Graphpad Software Inc., San Diego, CA, USA).

    Western Blot:

    Article Title: Elevation of Cytoplasmic Calcium Suppresses Microtentacle Formation and Function in Breast Tumor Cells
    Article Snippet: Densitometry analysis was conducted across 3 independent experiments using ImageJ software. .. Original immunoblot images and additional detailed densitometry method and analysis using the iBright Software (ThermoFisher, Waltham, MA, USA) can be found in the . .. All confocal imaging was conducted on tethered and fixed cells using an Olympus IX81 microscope with a FV-1000 confocal laser scanning system with a 60× oil emersion magnification and objective lens numerical aperture of 1.42.



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    Image Search Results


    Journal: The EMBO Journal

    Article Title: Varicella‐Zoster virus ORF9 is an antagonist of the DNA sensor cGAS

    doi: 10.15252/embj.2021109217

    Figure Lengend Snippet:

    Article Snippet: Web‐based iBright Image Analysis software , Thermo Fisher , .

    Techniques: Generated, Recombinant, Expressing, Flow Cytometry, Sequencing, Cloning, Labeling, Luciferase, Staining, Protease Inhibitor, Reverse Transcription, Software