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silica based weak cation exchanger polycat a  (PolyLC INC)

 
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    Structured Review

    PolyLC INC silica based weak cation exchanger polycat a
    Silica Based Weak Cation Exchanger Polycat A, supplied by PolyLC INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/weak+cation+exchanger+array/weak+cation+exchange+column/pmc06220366-51-6-20
    Average 90 stars, based on 1 article reviews
    silica based weak cation exchanger polycat a - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: VerteBrain reveals novel neural and non-neural protein assemblies conserved across vertebrate evolution
    Article Snippet: Three columns, each 200 3 4.6 mm ID, particle diameter 5 mm, pore diameter 100 A °, were connected in series in the following order: two PolyWAX LP columns followed by a single PolyCAT A (PolyLC, Inc, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies
    Article Snippet: Hb tetramers from RBCs were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Recombinant lentiviral vector for stem cell- based gene therapy of sickle cell disorder
    Article Snippet: Hemoglobin tetramers from mature erythroblasts were separated using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies.
    Article Snippet: Hb tetramers from RBCs were separated by CE HPLC using a 2-cation exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Extracellular Vesicle Size Reveals Cargo Specific to Coagulation and Inflammation in Pediatric and Adult Sickle Cell Disease
    Article Snippet: To determine the relative concentration of hemoglobin isoforms, hemolysates were subjected to weak cation-exchange high performance liquid chromatography (HPLC; Waters e2695; Waters Corporation, Milford, MA, USA), using a weak cation-exchange column (50 mm x 4.6 mm, 3 μm particles, 1500 Å pore; PolyCAT ATM; PolyLC Inc., Columbia, MD, USA), and eluted with a flow rate of 1.2 mL/min at 23°C using a linear gradient of phase B from 5% to 100% (mobile phase A: 20 mM Bis-Tris, 2 mM KCN, pH 6.96; mobile phase B: 20 mM Bis-Tris, 2 mM KCN, 200 mM NaCl, pH 6.55).

    Article Title: Design, Synthesis, and Investigation of Novel Nitric Oxide (NO)-Releasing Aromatic Aldehydes as Drug Candidates for the Treatment of Sickle Cell Disease.
    Article Snippet: For the Hb modification study, hemolysates from the above antisickling studies were subjected to HPLC (Hitachi D-7000 Series, Hitachi Instruments, Inc., San Jose, CA, USA) using a weak cation-exchange column (Poly CAT A: 35 mm × 4.6 mm, Poly LC, Inc., Columbia, MD, USA) to determine adduct formation.

    Article Title: Severe inflammation and lineage skewing are associated with poor engraftment of engineered hematopoietic stem cells in patients with sickle cell disease.
    Article Snippet: Hemoglobin tetramers were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).

    High Performance Liquid Chromatography:

    Article Title: VerteBrain reveals novel neural and non-neural protein assemblies conserved across vertebrate evolution
    Article Snippet: Three columns, each 200 3 4.6 mm ID, particle diameter 5 mm, pore diameter 100 A °, were connected in series in the following order: two PolyWAX LP columns followed by a single PolyCAT A (PolyLC, Inc, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies
    Article Snippet: Hb tetramers from RBCs were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Recombinant lentiviral vector for stem cell- based gene therapy of sickle cell disorder
    Article Snippet: Hemoglobin tetramers from mature erythroblasts were separated using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies.
    Article Snippet: Hb tetramers from RBCs were separated by CE HPLC using a 2-cation exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Extracellular Vesicle Size Reveals Cargo Specific to Coagulation and Inflammation in Pediatric and Adult Sickle Cell Disease
    Article Snippet: To determine the relative concentration of hemoglobin isoforms, hemolysates were subjected to weak cation-exchange high performance liquid chromatography (HPLC; Waters e2695; Waters Corporation, Milford, MA, USA), using a weak cation-exchange column (50 mm x 4.6 mm, 3 μm particles, 1500 Å pore; PolyCAT ATM; PolyLC Inc., Columbia, MD, USA), and eluted with a flow rate of 1.2 mL/min at 23°C using a linear gradient of phase B from 5% to 100% (mobile phase A: 20 mM Bis-Tris, 2 mM KCN, pH 6.96; mobile phase B: 20 mM Bis-Tris, 2 mM KCN, 200 mM NaCl, pH 6.55).

    Article Title: Design, Synthesis, and Investigation of Novel Nitric Oxide (NO)-Releasing Aromatic Aldehydes as Drug Candidates for the Treatment of Sickle Cell Disease.
    Article Snippet: For the Hb modification study, hemolysates from the above antisickling studies were subjected to HPLC (Hitachi D-7000 Series, Hitachi Instruments, Inc., San Jose, CA, USA) using a weak cation-exchange column (Poly CAT A: 35 mm × 4.6 mm, Poly LC, Inc., Columbia, MD, USA) to determine adduct formation.

    Article Title: Severe inflammation and lineage skewing are associated with poor engraftment of engineered hematopoietic stem cells in patients with sickle cell disease.
    Article Snippet: Hemoglobin tetramers were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).

    Modification:

    Article Title: VerteBrain reveals novel neural and non-neural protein assemblies conserved across vertebrate evolution
    Article Snippet: Three columns, each 200 3 4.6 mm ID, particle diameter 5 mm, pore diameter 100 A °, were connected in series in the following order: two PolyWAX LP columns followed by a single PolyCAT A (PolyLC, Inc, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies
    Article Snippet: Hb tetramers from RBCs were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Recombinant lentiviral vector for stem cell- based gene therapy of sickle cell disorder
    Article Snippet: Hemoglobin tetramers from mature erythroblasts were separated using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia, MD).

    Article Title: Novel lentiviral vectors for gene therapy of sickle cell disease combining gene addition and gene silencing strategies.
    Article Snippet: Hb tetramers from RBCs were separated by CE HPLC using a 2-cation exchange column (PolyCAT A, PolyLC, Columbia).

    Article Title: Extracellular Vesicle Size Reveals Cargo Specific to Coagulation and Inflammation in Pediatric and Adult Sickle Cell Disease
    Article Snippet: To determine the relative concentration of hemoglobin isoforms, hemolysates were subjected to weak cation-exchange high performance liquid chromatography (HPLC; Waters e2695; Waters Corporation, Milford, MA, USA), using a weak cation-exchange column (50 mm x 4.6 mm, 3 μm particles, 1500 Å pore; PolyCAT ATM; PolyLC Inc., Columbia, MD, USA), and eluted with a flow rate of 1.2 mL/min at 23°C using a linear gradient of phase B from 5% to 100% (mobile phase A: 20 mM Bis-Tris, 2 mM KCN, pH 6.96; mobile phase B: 20 mM Bis-Tris, 2 mM KCN, 200 mM NaCl, pH 6.55).

    Article Title: Design, Synthesis, and Investigation of Novel Nitric Oxide (NO)-Releasing Aromatic Aldehydes as Drug Candidates for the Treatment of Sickle Cell Disease.
    Article Snippet: For the Hb modification study, hemolysates from the above antisickling studies were subjected to HPLC (Hitachi D-7000 Series, Hitachi Instruments, Inc., San Jose, CA, USA) using a weak cation-exchange column (Poly CAT A: 35 mm × 4.6 mm, Poly LC, Inc., Columbia, MD, USA) to determine adduct formation.

    Article Title: Severe inflammation and lineage skewing are associated with poor engraftment of engineered hematopoietic stem cells in patients with sickle cell disease.
    Article Snippet: Hemoglobin tetramers were separated by cation exchange (CE)-HPLC using a 2 cation-exchange column (PolyCAT A, PolyLC, Columbia).



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    L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on <t>CM10</t> array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).
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    Image Search Results


    L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on CM10 array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).

    Journal: Proteome Science

    Article Title: S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells

    doi: 10.1186/1477-5956-9-76

    Figure Lengend Snippet: L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on CM10 array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).

    Article Snippet: Weak cation-exchange ProteinChip CM10 arrays (Bio-Rad Laboratories, Hercules, CA, USA) were used for protein profiling.

    Techniques: Selection, Expressing

    Characterization of the 11.1 kDa protein . (A) Estimation of pI of the 11.1 kDa protein. The affinity of the 11.1 kDa protein (indicated by the arrow) to a CM10 array changed with a pH range of 3.0-10.0. (B) Estimation of experimental molecular mass of the 11.1 kDa protein (indicated by the arrow). Bovine insulin (5733.5 Da) and equine cytochrome C (12361.0 Da) were calibrants for internal calibration.

    Journal: Proteome Science

    Article Title: S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells

    doi: 10.1186/1477-5956-9-76

    Figure Lengend Snippet: Characterization of the 11.1 kDa protein . (A) Estimation of pI of the 11.1 kDa protein. The affinity of the 11.1 kDa protein (indicated by the arrow) to a CM10 array changed with a pH range of 3.0-10.0. (B) Estimation of experimental molecular mass of the 11.1 kDa protein (indicated by the arrow). Bovine insulin (5733.5 Da) and equine cytochrome C (12361.0 Da) were calibrants for internal calibration.

    Article Snippet: Weak cation-exchange ProteinChip CM10 arrays (Bio-Rad Laboratories, Hercules, CA, USA) were used for protein profiling.

    Techniques: