sepharose column (Danaher Inc)
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Danaher Inc
sepharose column
Sepharose Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/weak+cation+exchanger+array/HiTrap+CM+FF/pmc05063504-73-10-15
Average 93 stars, based on 77 article reviews
Sepharose Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/weak+cation+exchanger+array/HiTrap+CM+FF/pmc05063504-73-10-15
Average 93 stars, based on 77 article reviews
sepharose column - by Bioz Stars,
2026-11
93/100 stars
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Centrifugation:Article Title: Arabidopsis AtPME2 has a pH-dependent processivity and control cell wall mechanical properties Article Snippet: .. Culture supernatants recovered following centrifugation, were applied onto a Western Blot:Article Title: Bacterial-type ferroxidase tunes iron-dependent phosphate sensing during Arabidopsis root development Article Snippet: The resulting protein pellet was solubilized in 2-3 ml buffer A, loaded on a HighLoad Superdex 200 gel filtration column (HL 16/60, GE Healthcare), and eluted with buffer A as the mobile phase. .. Fractions containing LPR1 (detected by immunoblot analysis) were directly applied to Recombinant:Article Title: Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS. Article Snippet: Expression of the partial recombinant protein of the LymCBP catalytic domain was induced by treatment with 0.32 mM of isopropyl β- d- thiogalactopyranoside (IPTG) in TBG- M9 medium [0.8% Bacto Tryptone (BD) and 0.4% NaCl], followed by purification using nickel- nitrilotriacetic acid (Ni- NTA) agarose resin (QIAGEN). .. The recombinant protein was desalted with a PD- 10 column (SephadexTM G- 25 M, GE Healthcare), purified using a Article Title: Histone acetyltransferase activity of CREB-binding protein is essential for synaptic plasticity in Lymnaea Article Snippet: Expression of the partial recombinant protein of the LymCBP catalytic domain was induced by treatment with 0.32 mM of isopropyl β-D-thiogalactopyranoside (IPTG) in TBG-M9 medium [0.8% Bacto Tryptone (BD) and 0.4% NaCl], followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). .. The recombinant protein was desalted with PD-10 column (Sephadex TM G-25M, GE Healthcare), purified using a Purification:Article Title: Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS. Article Snippet: Expression of the partial recombinant protein of the LymCBP catalytic domain was induced by treatment with 0.32 mM of isopropyl β- d- thiogalactopyranoside (IPTG) in TBG- M9 medium [0.8% Bacto Tryptone (BD) and 0.4% NaCl], followed by purification using nickel- nitrilotriacetic acid (Ni- NTA) agarose resin (QIAGEN). .. The recombinant protein was desalted with a PD- 10 column (SephadexTM G- 25 M, GE Healthcare), purified using a Article Title: Histone acetyltransferase activity of CREB-binding protein is essential for synaptic plasticity in Lymnaea Article Snippet: Expression of the partial recombinant protein of the LymCBP catalytic domain was induced by treatment with 0.32 mM of isopropyl β-D-thiogalactopyranoside (IPTG) in TBG-M9 medium [0.8% Bacto Tryptone (BD) and 0.4% NaCl], followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). .. The recombinant protein was desalted with PD-10 column (Sephadex TM G-25M, GE Healthcare), purified using a other:Article Title: Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: insights into plasticity of substrate binding and activation Article Snippet: Four consecutive chromatography steps were used in the purification process: (1) anion exchange chromatography (5 ml HiTrap Q FF column, GE Healthcare; gradient elution with NaCl in 20 mM Tris–HCl pH 8.0 buffer), (2) affinity chromatography (5 ml Maltose-Binding-Protein/MBPTrap HP column, GE Healthcare; isocratic elution with 150 mM NaCl in 20 mM Tris–HCl pH 8.0 buffer), (3) cation exchange chromatography (1 ml HiTrap CM FF column, GE Healthcare; gradient elution with NaCl in 50 mM acetate buffer pH 5.0) and (4) size exclusion chromatography (HiLoad Superdex75 16/60 column, GE Healthcare; isocratic elution with 150 mM NaCl in 20 mM Tris–HCl pH 8.0 buffer). |
