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cm10 (weak cation exchange array  (Bio-Rad)


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    Bio-Rad cm10 (weak cation exchange array
    Cm10 (Weak Cation Exchange Array, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/weak+cation+exchanger+array/cm10/pm21145706-68-15-11
    Average 90 stars, based on 1 article reviews
    cm10 (weak cation exchange array - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Isolation and Proteomic Analysis of Platelets by SELDI-TOF MS
    Article Snippet: CM10 Low-Stringency Binding Buffer (Bio-Rad).

    Article Title: Identification of diagnostic upper gastrointestinal cancer tissue type-specific urinary biomarkers
    Article Snippet: Urine (0.5 ml) from positive or negative samples in relation to specific peaks was added to 30 μl CM10 or IMAC30 (Cu 2+ -complexed) spin column resin (Bio-Rad Laboratories, Ltd.) and 0.75 ml binding buffer [0.1 M NaHPO 4 (pH 4.0) for CM10 resins, and 0.1 M NaHPO 4 (pH 7.0) including 0.5 M NaCl for IMAC30 resins] and incubated for 1 h at room temperature under constant agitation.

    Article Title: Detection of Colorectal Cancer by Serum and Tissue Protein Profiling: A Prospective Study in a Population at Risk
    Article Snippet: All serum samples were analysed with SELDI-TOF MS (Biorad Laboratories, Hercules, CA, U.S.A.) on CM10 chips as described previously ( ).

    Article Title: Evaluation of Molecular Species of Prostate-Specific Antigen Complexed with Immunoglobulin M in Prostate Cancer and Benign Prostatic Hyperplasia
    Article Snippet: ProteinChip: PS20 (preactivated surface), Q10 (strong anion exchanger), CM10 (weak cation exchanger), sinapinic acid, and ProteinChip all-in-one protein standards II were from Bio-Rad (Hercules, CA, USA).

    Article Title: Isolation and Proteomic Analysis of Platelets by SELDI-TOF MS
    Article Snippet: There are other chemistries available such as Immobilized Metal Affinity Capture (IMAC30) and Hydrophobic (H50) and the corresponding buffers, available from Bio-Rad, should be used in conjunction with the other chemistries. list-behavior=enumeratedprefix-word=mark-type=decimalmax-label-size=0 Equilibrate CM10 ProteinChip Arrays with 150 μL CM10 Low Stringency Buffer for 5 min with vigorous shaking at room temperature.

    Binding Assay:

    Article Title: Urinary diagnostic proteomic markers for dynapenia in cancer patients
    Article Snippet: .. Urine (0.5 ml) from positive or negative samples in relation to specific peaks was added to 30 ml CM10 or IMAC30 spin column resins (Bio-Rad) and 0.75 ml binding buffer [0.1 M NaHPO 4 (pH 4.0) for CM10 resins, and or 0.1 M NaHPO 4 (pH 7.0) including 0.5 M NaCl for IMAC30 resins] and incubated for 1 h at room temperature under constant agitation. ..

    Incubation:

    Article Title: Urinary diagnostic proteomic markers for dynapenia in cancer patients
    Article Snippet: .. Urine (0.5 ml) from positive or negative samples in relation to specific peaks was added to 30 ml CM10 or IMAC30 spin column resins (Bio-Rad) and 0.75 ml binding buffer [0.1 M NaHPO 4 (pH 4.0) for CM10 resins, and or 0.1 M NaHPO 4 (pH 7.0) including 0.5 M NaCl for IMAC30 resins] and incubated for 1 h at room temperature under constant agitation. ..



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    L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on <t>CM10</t> array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).
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    Image Search Results


    L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on CM10 array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).

    Journal: Proteome Science

    Article Title: S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells

    doi: 10.1186/1477-5956-9-76

    Figure Lengend Snippet: L-OHP sensitivity and candidate peak selection . (A) Protein expression profiles of each cell line on CM10 array at pH 4.5. The candidate peak is enclosed by the rectangle. (B) Peak intensity of the 11.1 kDa protein in 11 CRC cell lines strongly correlates with L-OHP sensitivity. The peak intensity and IC 50 value of each cell line are plotted as means ± S.D. (peak intensity, n = 3; IC 50 , n = 3 or 4).

    Article Snippet: Weak cation-exchange ProteinChip CM10 arrays (Bio-Rad Laboratories, Hercules, CA, USA) were used for protein profiling.

    Techniques: Selection, Expressing

    Characterization of the 11.1 kDa protein . (A) Estimation of pI of the 11.1 kDa protein. The affinity of the 11.1 kDa protein (indicated by the arrow) to a CM10 array changed with a pH range of 3.0-10.0. (B) Estimation of experimental molecular mass of the 11.1 kDa protein (indicated by the arrow). Bovine insulin (5733.5 Da) and equine cytochrome C (12361.0 Da) were calibrants for internal calibration.

    Journal: Proteome Science

    Article Title: S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells

    doi: 10.1186/1477-5956-9-76

    Figure Lengend Snippet: Characterization of the 11.1 kDa protein . (A) Estimation of pI of the 11.1 kDa protein. The affinity of the 11.1 kDa protein (indicated by the arrow) to a CM10 array changed with a pH range of 3.0-10.0. (B) Estimation of experimental molecular mass of the 11.1 kDa protein (indicated by the arrow). Bovine insulin (5733.5 Da) and equine cytochrome C (12361.0 Da) were calibrants for internal calibration.

    Article Snippet: Weak cation-exchange ProteinChip CM10 arrays (Bio-Rad Laboratories, Hercules, CA, USA) were used for protein profiling.

    Techniques: