fdss waveform analysis software for cardiomyocytes (Hamamatsu)
90
Structured Review
Hamamatsu
fdss waveform analysis software for cardiomyocytes
Fdss Waveform Analysis Software For Cardiomyocytes, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/waveform+analysis+software/fdss+waveform+analysis+software+for+cardiomyocytes/pmc06431403-62-49-51
Average 90 stars, based on 1 article reviews
Fdss Waveform Analysis Software For Cardiomyocytes, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/waveform+analysis+software/fdss+waveform+analysis+software+for+cardiomyocytes/pmc06431403-62-49-51
Average 90 stars, based on 1 article reviews
fdss waveform analysis software for cardiomyocytes - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients"
Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients
Journal: Stem Cells International
doi: 10.1155/2019/7532657
Figure Legend Snippet: Patch clamp analysis of cardiomyocytes from control- and LQT-iPSC lines. (a) Representative APs of 1 Hz paced a control-iPSC-CM and an LQT-iPSC-CM from II-2 in . Ten consecutive waves are shown. (b) MDP, APA, and APD 90 from cardiomyocytes derived from the six lines: 201B7 ( n = 6), 409B2 ( n = 5), 692D2 ( n = 6), LQT1A1 ( n = 5), LQT1B1 ( n = 5), and LQT1C1 ( n = 7). Data are represented as means ± SEM; ∗∗ p < 0.005. (c) Representative current traces from control- and LQT1-iPSC-CMs. Upper, the protocol in current clamp recording. Middle, representative traces before and after perfusion with 3R4S-chromanol 293B (30 μ mol/l). Lower, 3R4S-chromanol 293B-subtraction. (d) I-V plots of I Ks at the end of the depolarizing step. 692D2 (control) ( n = 3), LQT1B1 ( n = 3); ∗ p < 0.05.
Techniques Used: Patch Clamp, Control, Derivative Assay
Figure Legend Snippet: AP measurements of single cells using a membrane potential dye. (a) Examples of AP waves from control (409B2) measured by the dye. ROIs (1)–(5) are ventricular type. ROI (5) (green wave) shows long APD. (b) Examples of AP waves from LQT1B1. ROIs (1)–(4) are ventricular type with long APD. ROI (4) (purple wave) shows EADs. ROI (5) (green wave) is nodal type. (c) Proportion of subtypes in 3 control- and 3 LQT1-iPSC-CMs: 201B7 ( n = 128), 409B2 ( n = 41), 692D2 ( n = 92), LQT1A1 ( n = 192), LQT1B1 ( n = 101), and LQT1C1 ( n = 202). Ventricular-type cells predominate in all six lines. (d) Representative EADs in a ventricular-type cell from LQT1B1. The EADs were defined as over 20% of the AP amplitude. Arrows show EADs. (e) Occurrence ratio of EADs in ventricular cardiomyocytes from the six lines. The number of ventricular cells was used as the denominator; ∗ p < 0.05.
Techniques Used: Membrane, Control
Figure Legend Snippet: AP measurements in a cardiomyocyte monolayer. Data are represented as means ± SEM. (a) An example phase contrast image of the monolayer culture. Scale bar, 500 μ m. (b) Representative AP of a paced control-iPSC-CM monolayer. APD 90 was calculated from the average of 10 consecutive waves. (c) APD 90 from 3 control- and 3 LQT-iPSC-CM monolayers: 201B7 ( n = 3), 409B2 ( n = 3), 692D2 ( n = 3), LQT1A1 ( n = 3), LQT1B1 ( n = 3), and LQT1C1 ( n = 3). LQT-iPSC-CMs showed longer APD than controls; ∗∗ p < 0.005.
Techniques Used: Control
Figure Legend Snippet: AP measurements in cardiomyocyte monolayers using a high-throughput plate reader. (a) Representative AP waves on a 96-well plate. Pacing at 1 Hz starts 10 s after recording. (b) Cisapride prolonged APD 90 of both control- and LQT1-iPSC-CMs; 692D2 (control) ( n = 3), LQT1B1 ( n = 5). (c) Cisapride prolonged APD 90 of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 ( n = 5), LQT1B1 ( n = 3). (d) Erythromycin prolonged APD 90 of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 ( n = 4), LQT1B1 ( n = 3). LQT-iPSC-CM monolayers in 300 nM and 1000 nM cisapride were not synchronized at 1 Hz pacing. Data are represented as means ± SEM; ∗∗ p < 0.005, ∗ p < 0.05.
Techniques Used: High Throughput Screening Assay, Control
Related Articles
Software:Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients Article Snippet: An output excitation wavelength was used with standard settings of 492 BP 20 nm and emission filter 540AF40 (Omega Optical, Brattleboro, VT, USA). .. Binning was set to 4 × 4, and the measurement interval was every 4 ms. Cardiomyocyte monolayers 7–10 days after seeding on each well were stimulated at 1 Hz with 1 ms depolarizing pulses at 10 V. Analysis of APD 90 was performed with |
