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revert 700 wash solution (LI-COR)

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LI-COR revert 700 wash solution
Revert 700 Wash Solution, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wash+solution/REVERT+700+WASH+SOLUTION/pmc12907856-91-4-13
Average 96 stars, based on 54 article reviews

revert 700 wash solution - by Bioz Stars, 2026-11

96/100 stars

Images

Staining:

Article Title: Low concentrations of amyloid-beta oligomers induce synaptogenesis characteristic for mild cognitive impairment and alter the de novo proteome.
Article Snippet: After transfer, the membranes were air-dried, rehydrated with ultrapure water, and stained with Revert 700 Total Protein Stain (LI-COR) for 5 minutes. .. Excess stain was removed using Revert 700 Wash Solution (LI-COR), followed by rinsing with ultrapure water. ..

Article Title: Low concentrations of amyloid-beta oligomers induce synaptogenesis characteristic for mild cognitive impairment and alter the de novo proteome
Article Snippet: After transfer, the membranes were air-dried, rehydrated with ultrapure water, and stained with Revert 700 Total Protein Stain (LI-COR) for 5 min. .. Excess stain was removed using Revert 700 Wash Solution (LI-COR), followed by rinsing with ultrapure water. ..

other:

Article Title: A post-translational regulatory map of chronic antigen-driven human T cell dysfunction
Article Snippet: On the day of the analysis, frozen cell pellets were thawed on ice, resuspended in ice-cold PBS containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (Roche) and lysed by sonication (3 x 8 pulses, 60% duty cycle).

Article Title: PhuS conformational dynamics are essential for DNA binding and heme-responsive control of the prrF operon in Pseudomonas aeruginosa
Article Snippet: Cell pellets were lysed in BugBuster HT reagent (Sigma Aldrich) on a rotating platform for 20 min at 25 o C and total protein concentration was determined by DC protein assay (Bio-Rad).

Article Title: PhuS conformational dynamics are essential for DNA binding and heme-responsive control of the prrF operon in Pseudomonas aeruginosa.
Article Snippet: 10 μg of total protein was added to 3 μL of 4X LICOR Loading buffer (LICORbio), boiled for 5 min at 95 ̊C, and ran on a 10% polyacrylamide gel in Tris-Glycine-SDS buffer (pH 8.3, Biorad) at 3 mA for 2 h, alongside the Chameleon duo prestained protein ladder (LI-COR).

Imaging:

Article Title: Multi-omics profiling reveals MAGEL2-driven defects in human corticogenesis shared across Prader-Willi and Schaaf-Yang syndromes
Article Snippet: For total protein normalization, membranes were rinsed with ultrapure water and stained with RevertTM 700 Total Protein Stain (LI-COR #926-11010) for 5 min before the blocking step. .. Membranes were washed twice with RevertTM Wash Solution (LI-COR #926-11012) and imaged in the 700 nm channel using an Odyssey Imaging System (LI-COR Biosciences). .. Following total protein imaging, membranes were rinsed with PBST and blocked with 5% Milkpowder (blotting grade, Carl Roth #T145) in PBST for 1 hr at RT and incubated with following primary antibodies overnight at 4°C, anti-FAK (1:1000, Abcam #ab40794) and anti-pFAK (Tyr397; 1:2000 Cell Signaling Technology #8556.

Western Blot:

Article Title: Activity-based CO 2 sensing using CarboSenR2 provides new insights into cellular metabolism
Article Snippet: Wet transfer was performed onto nitrocellulose membranes and reversibly stained with Revert 700 total protein stain (LI-COR, Lincoln, NE, USA). .. Membranes were washed in Revert 700 wash solution and imaged at 700 nm (LI-COR), then destained in Revert 700 destaining solution (Li-Cor) prior to immunoblotting. .. Membranes were blocked in 5% milk in TBST for 1 h followed by overnight incubation in primary antibody (1:1000) at 4 °C (phospho Acetyl-CoA Carboxylase (Ser79) (CST-3661S, Cell Signalling), PGC-1a (A12348, ABclonal), Lamin A/C (CST-4777S, Cell Signalling), mitochondrial cytochrome c oxidase subunit 1 (ab14705, Abcam)).