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washing solution  (Thermo Fisher)


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    Thermo Fisher washing solution
    Washing Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+solution/Hanks'+Balanced+Salt+Solution+(1X)%2C+without+calcium%2C+without+magnesium%2C+without+phenol+red/pm41997233-65-7-13
    Average 97 stars, based on 1 article reviews
    washing solution - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Bacteria:

    Article Title: Hyperinvasive and quinolone-resistant meningococci cc4821 achieved immune evasion through capsule switching.
    Article Snippet: Approximately 20 colonies of freshly inoculated strains were plated for confluent growth onto Columbia horse blood agar plates (CHBA) (Thermofisher, USA), and incubated at 37 °C with 5% CO2 for four hours. .. The bacteria were then suspended in Hank's Balanced Salts (without Ca2+or Mg2+) (Gibco, USA) supplemented with 0.1% glucose, adjusting the suspension to 6-10 × 104 CFU/ml. .. The bacterial suspension ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS was mixed with active rabbit complement (Dynal, USA) at a ratio of 1:1, and 20 μl of the mixture was added to each well of a round-bottom 96-well microplate containing 20 μl of serially twofold diluted serum.

    Suspension:

    Article Title: Hyperinvasive and quinolone-resistant meningococci cc4821 achieved immune evasion through capsule switching.
    Article Snippet: Approximately 20 colonies of freshly inoculated strains were plated for confluent growth onto Columbia horse blood agar plates (CHBA) (Thermofisher, USA), and incubated at 37 °C with 5% CO2 for four hours. .. The bacteria were then suspended in Hank's Balanced Salts (without Ca2+or Mg2+) (Gibco, USA) supplemented with 0.1% glucose, adjusting the suspension to 6-10 × 104 CFU/ml. .. The bacterial suspension ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS was mixed with active rabbit complement (Dynal, USA) at a ratio of 1:1, and 20 μl of the mixture was added to each well of a round-bottom 96-well microplate containing 20 μl of serially twofold diluted serum.

    Incubation:

    Article Title: Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1.
    Article Snippet: For transfection, cells were transfected with PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions and incubated in serum-free DMEM overnight (serum starvation). .. The next day, cells were incubated for 2 h in modified Hanks’ balanced salt solution (1× HBSS, diluted from 10× HBSS; 14065, Gibco) and supplemented with 20 mM HEPES pH 7.4 and 2 g L−1 glucose at 37 °C (referred to as ‘double starvation’) before live-cell imaging. ..

    Modification:

    Article Title: Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1.
    Article Snippet: For transfection, cells were transfected with PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions and incubated in serum-free DMEM overnight (serum starvation). .. The next day, cells were incubated for 2 h in modified Hanks’ balanced salt solution (1× HBSS, diluted from 10× HBSS; 14065, Gibco) and supplemented with 20 mM HEPES pH 7.4 and 2 g L−1 glucose at 37 °C (referred to as ‘double starvation’) before live-cell imaging. ..

    Article Title: Chitosan-Stabilized Ferric Oxyhydroxide Nanoparticles: A Food-Compatible Vehicle for Efficient Intestinal Iron Delivery
    Article Snippet: 2-(N-morpholino)-ethanesulfonic acid (MES) was obtained from Shanghai Sangong Biological Co., Ltd. (Shanghai, China). .. Dulbecco’s Modified Eagle Medium (DMEM), Hank's balanced salt solution (HBSS) without phenol red, fetal bovine serum (FBS), and trypsin EDTA solution were obtained from Gibco Life Sciences Ltd. (Thermo Fisher Scientific, USA). .. Ultrapure water with a resistivity exceeding 18 MΩ·cm, obtained from a Milli-Q Plus purification unit (Merck Millipore, Göttingen, Germany), was utilized for every experimental purpose, including sample preparation, cleaning of glassware, and animal hydration.

    Live Cell Imaging:

    Article Title: Genetically targeted mTORC1 inhibitor reveals transcriptional control by nuclear mTORC1.
    Article Snippet: For transfection, cells were transfected with PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions and incubated in serum-free DMEM overnight (serum starvation). .. The next day, cells were incubated for 2 h in modified Hanks’ balanced salt solution (1× HBSS, diluted from 10× HBSS; 14065, Gibco) and supplemented with 20 mM HEPES pH 7.4 and 2 g L−1 glucose at 37 °C (referred to as ‘double starvation’) before live-cell imaging. ..

    other:

    Article Title: NK cell dysfunction and interferon-γ production underlie autoinflammation in mevalonate kinase deficiency.
    Article Snippet: Isolation and culture of murine cells Whole bone marrow was flushed with PBS (Gibco) from the femur and tibia.



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