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wash buffer  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL wash buffer
    Wash Buffer, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+buffer/Wash+Buffer+WASH/pmc09249600-99-4-6
    Average 93 stars, based on 16 article reviews
    wash buffer - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Affinity Chromatography:

    Article Title: Identification of small molecule inhibitors of Trypanosoma PEX15– PEX6 interaction
    Article Snippet: .. Reverse nickel affinity chromatography with wash buffer and elution buffer I was performed using a 5 mL Ni-NTA column (Protino, Macherey-Nagel) for removal of TEV-protease followed by size exclusion chromatography using HEPES buffer. ..

    Size-exclusion Chromatography:

    Article Title: Identification of small molecule inhibitors of Trypanosoma PEX15– PEX6 interaction
    Article Snippet: .. Reverse nickel affinity chromatography with wash buffer and elution buffer I was performed using a 5 mL Ni-NTA column (Protino, Macherey-Nagel) for removal of TEV-protease followed by size exclusion chromatography using HEPES buffer. ..

    DNA Purification:

    Article Title: Sequencing, de novo assembly of Ludwigia plastomes, and comparative analysis within the Onagraceae family
    Article Snippet: DNA was then precipitated after addition of an equal volume of binding buffer C4 (Macherey-Nagel ) and 99% ethanol overnight at 4°C or 1 h in ice then centrifuged at 800 rpm for 10 min. After removal of the supernatant, 1 ml of CQW buffer was added then the pellet of DNA was re-suspended. .. Next, DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (Macherey-Nagel), wash buffer B5 (Macherey-Nagel), and ethanol at 99% in equal volumes, followed by centrifugation at 800 rpm for 10 min. ..

    Article Title: Sequencing, de novo assembly of Ludwigia plastomes, and comparative analysis within the Onagraceae family
    Article Snippet: DNA was then precipitated after addition of an equal volume of binding buffer C4 (MACHEREY-NAGEL) and 99% ethanol overnight at 4°C or 1 h in ice then centrifuged at 800 rpm for 10 min. After removal of the supernatant, 1 ml of CQW buffer was added then the pellet of DNA was re-suspended. .. Next, DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL), and ethanol at 99% in equal volumes, followed by centrifugation at 800 rpm for 10 min. ..

    Centrifugation:

    Article Title: Sequencing, de novo assembly of Ludwigia plastomes, and comparative analysis within the Onagraceae family
    Article Snippet: DNA was then precipitated after addition of an equal volume of binding buffer C4 (Macherey-Nagel ) and 99% ethanol overnight at 4°C or 1 h in ice then centrifuged at 800 rpm for 10 min. After removal of the supernatant, 1 ml of CQW buffer was added then the pellet of DNA was re-suspended. .. Next, DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (Macherey-Nagel), wash buffer B5 (Macherey-Nagel), and ethanol at 99% in equal volumes, followed by centrifugation at 800 rpm for 10 min. ..

    Article Title: Sequencing, de novo assembly of Ludwigia plastomes, and comparative analysis within the Onagraceae family
    Article Snippet: DNA was then precipitated after addition of an equal volume of binding buffer C4 (MACHEREY-NAGEL) and 99% ethanol overnight at 4°C or 1 h in ice then centrifuged at 800 rpm for 10 min. After removal of the supernatant, 1 ml of CQW buffer was added then the pellet of DNA was re-suspended. .. Next, DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL), and ethanol at 99% in equal volumes, followed by centrifugation at 800 rpm for 10 min. ..

    Amplification:

    Article Title: Engineering Antigen-Specific Tolerance to an Artificial Protein Hydrogel
    Article Snippet: .. The plasmids were amplified in E. coli DH10B and purified by Maxiprep with endotoxin-free wash buffer (Macherey-Nagel). ..

    Article Title: Engineering Antigen-Specific Tolerance to an Artificial Protein Hydrogel
    Article Snippet: .. The plasmids were amplified in E. coli, purified by Maxiprep with endotoxin-free wash buffer (Macherey-Nagel), complexed with ExpiFectamine CHO Reagent (ThermoFisher), and used to transfect ExpiCHO-S cells (ThermoFisher) following the manufacturer’s instructions. .. Within two days, transfected suspension cells were transitioned into 364-well plates containing fresh ExpiCHO Expression Media supplemented with Hygromycin B (100 μg/mL), 10% FBS, and 2.5 μg/mL Fungizone (Amphotericin B, ThermoFisher).

    Purification:

    Article Title: Engineering Antigen-Specific Tolerance to an Artificial Protein Hydrogel
    Article Snippet: .. The plasmids were amplified in E. coli DH10B and purified by Maxiprep with endotoxin-free wash buffer (Macherey-Nagel). ..

    Article Title: Engineering Antigen-Specific Tolerance to an Artificial Protein Hydrogel
    Article Snippet: .. The plasmids were amplified in E. coli, purified by Maxiprep with endotoxin-free wash buffer (Macherey-Nagel), complexed with ExpiFectamine CHO Reagent (ThermoFisher), and used to transfect ExpiCHO-S cells (ThermoFisher) following the manufacturer’s instructions. .. Within two days, transfected suspension cells were transitioned into 364-well plates containing fresh ExpiCHO Expression Media supplemented with Hygromycin B (100 μg/mL), 10% FBS, and 2.5 μg/mL Fungizone (Amphotericin B, ThermoFisher).

    Lysis:

    Article Title: Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR-Cas9 Mediated TcNPR3 Mutagenesis.
    Article Snippet: .. The sorbitol wash step was then repeated, followed by the addition of 5 mL lysis buffer to the supernatant and the extraction of HMW DNA using the NucleoBond HMW DNA kit (Macherey- Nagel, 740160.20). .. The samples were submitted to the Huck Institutes Genomics Core Facility at Penn State University, and their quality was evaluated using an Agilent TapeStation 4150.

    Extraction:

    Article Title: Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR-Cas9 Mediated TcNPR3 Mutagenesis.
    Article Snippet: .. The sorbitol wash step was then repeated, followed by the addition of 5 mL lysis buffer to the supernatant and the extraction of HMW DNA using the NucleoBond HMW DNA kit (Macherey- Nagel, 740160.20). .. The samples were submitted to the Huck Institutes Genomics Core Facility at Penn State University, and their quality was evaluated using an Agilent TapeStation 4150.



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